Sentences with phrase «incubating cultured cells»

Incubating cultured cells with RK - 33 had a similar effect, knocking down DDX3 expression in cells that highly express this protein and hampering their ability to multiply.

Not exact matches

Animal cells are cultured from stem cells and incubated in a «bioreactor» into tissue that can be «harvested» and formed into familiar foods like meatballs, patties, and fish sticks.

The team pumped around 50 million rat liver cells into each of five bare scaffolds, then incubated the organs in culture for two weeks.

The research builds on the team's previous work with a technique called three - dimensional culture, which involves incubating stem cells in a floating ball - shaped aggregate, unlike traditional cell culture in which cells grow in a flat layer on the surface of a culture dish.

The group reported growing multiple parthenogenetic embryonic stem cell lines by incubating eggs in a warm, low - oxygen culture medium.

Specifically, cell cultures from bone tissue were incubated in 10 % DMEM containing 10 ng / ml TNF - α and 10 μg / ml fluorescent probe of acetylated LDL, DiI - Ac - LDL (Biochemical Technologies, Cambridge, MA), for 4 h. Cells were harvested in the manner described above with the exception that during this labeling period, the rat anti-E-selectin mAb (10E9.6) conjugated to FITC replaced PE - conjugated 10E9.6.

Cells (5 × 104) were seeded in 12.5 cm2 culture flask and incubated for 24 h to allow for cell attachment and recovery.

Cells were then plated in 24 - well tissue culture treated plates and incubated in a humidified 5 % CO2 incubator at 37 °C.

For adenoviral transduction, HB1.F3 cells were incubated with purified adenovirus (1 ∶ 1000 dilution in DMEM) for 4 h and then cultured overnight in DMEM.

Therefore, twelve day old, DMSO - differentiated, Ara - C treated P19 cultures were incubated with the proteasome inhibitors MG - 132 and lactacystin for four hours; this was sufficient to rescue Oct4 protein expression (Figure 1D), suggesting that these cells do indeed synthesize new Oct4 protein.

After growing to a 60 % to 70 % confluence level, cells were arrested with 1 ml of Colcemid solution (10 µg / ml)(Invitrogen) for every 50 ml cell culture and incubated at 37 °C for 2 hours.

The researchers took the leaf extracts by taking several cell cultures and incubated each of them with various concentrations of stinging nettle preparations, whilst adding TNF to stimulate inflammation.