All functional analysis was done using
Ingenuity Pathway Analysis with the default parameters as it was shown to be a reliable approach to identify biological pathways that influence disease outcomes [52].
Pathway Analysis of the data was performed with
Ingenuity Pathways Analysis (Ingenuity Systems, www.ingenuity.com).
We found that the complement cascade related proteins were identified and significantly enriched in both CFS and nPTLS pooled CSF proteomes by the Fisher Exact test (p = 0.005) implemented in
Ingenuity Pathways Analysis (Figure S1A).