Sentences with phrase «k563 cell lysis»

The distinction between apoptosis and cell lysis is important in immunology - lysing a virus - infected cell would only release the virions, whereas apoptosis leads to destruction of the virus inside.

Reactions were terminated with 1 ml of ice - cold PBS, and cells were centrifuged and resuspended in 50 μl of cell lysis buffer containing 1 % NP - 40 and protease inhibitors.

Cell pellets were resuspended in 1 ml cell lysis buffer [5 mM Pipes pH 8.0, 85 mM KCl, 0.5 % NP - 40] containing protease inhibitors and incubated for 10 min on ice.

The cells were incubated for 15 — 30 minutes at room temperature to allow complete cell lysis before determining luminescent levels using in an Envision plate reader.

CONCLUSIONS: Our findings suggest that excessive intracellular accumulation of A beta 42 - positive material in pyramidal cells can result in cell lysis, and that cell lysis is an important source of amyloid plaques and neuronal loss in AD brains.

Vince guided them through cell lysis (soap), precipitation (salt), and then separation (ethanol).

2) Ensure cell lysis occurs quickly (within 2 mins) if removed from hypoxia.

Conversely, down - regulation of PKAc1 or stabilisation of a dominant - negative PKAr isoform that does not bind cAMP triggers premature parasite egress from infected cells followed by serial invasion attempts leading to host cell lysis.

They are not to be administered rapidly because of their potential to cause red blood cell lysis.

Not exact matches

As I mentioned before, our subconscious understanding of the laws of the universe does not question the perfection which allows us to breath, drink, eat, and procreate, it simply understands that perfection because ultimately every cell division, firing of a neuron, lysis of a cell, infection etc. is subject to those laws, whether we consciously see them as perfect or not.

Once the cells were trapped, researchers observed how they underwent lysis: their cell walls and membrane ruptured, the cytoplasm leaked, and the cell disintegrated.

Most of those errors stem from the cell - lysis protocols scientists have been using.

Thus, the two small phages characterized for their lysis strategy, Qβ and the small DNA phage φX174, effect host lysis by targeting different enzymes in the multistep, universally conserved pathway of cell wall biosynthesis.

If the Listeria cells are then brought to the point where they rupture (lysis), the bacteriophages are released and can be isolated and multiplied for use in therapy or diagnostics.

Carriage of HIV - 1 by dendritic cells may facilitate the lysis and loss of antigen specific CD4 + T cells in acquired immunodeficiency syndrome.

Previous studies have shown that C. trachomatis leaves a cell either via lysis or in the membrane - bound inclusion, which is extruded intact from the cell and may serve to protect C. trachomatis on its way to infect new cells.

Peripheral blood mononuclear cells were isolated by centrifugation with 96 % Ficoll (BD), followed by erythrocyte lysis with ACK lysis buffer.

At 48 hours, cells were washed twice with PBS and lysed in 1 × passive lysis buffer (Dual - Luciferase Reporter Assay, Promega).

Lane 1: HEK cells Lane 2: hfRPE cells Lane 3: hfRetina PVDF membrane RIPA lysis buffer with mixed phosphatase and protease inhibitors

Recently, we showed that bortezomib treatment in mice bearing breast and kidney tumors provided host survival benefit by amplifying tumor cell caspase8 activation, CD8T cell effector molecules via increased NotchNFkB crosstalk and Fas ligandmediated lysis of tumor cells.

Moretta A, Vitale M, Bottino C, Orengo AM, Morelli L, Augugliaro R, Barbaresi M, Ciccone E, Moretta L. P58 molecules as putative receptors for major histocompatibility complex (MHC) class I molecules in human natural killer (NK) cells: anti-p58 antibodies reconstitute lysis of MHC class I - protected cells in NK clones displaying different specificities.

Cytotoxicity was determined by the amount of 51Cr released by the target cells and the specific lysis was calculated as follows:

Spleens were processed into cell suspensions through a 70 - μm strainer by a syringe plunger in RPMI 1640 with 10 % FBS and then treated with ACK lysis buffer to remove RBCs.

Lane 1: HEK cells Lane 2: hfRPE cells Lane 3: hfRetina Lane 4: Mouse liver Lane 5: Mouse RPE Lane 6: Mouse retina Lane 7: Zebrafish liver Lane 8: Zebrafish retina PVDF membrane RIPA lysis buffer with mixed phosphatase and protease inhibitor cocktail

Tumors can suppress CD4 + T cell activity and CTL tumor lysis directly through secretion of immunosuppressive factors including TGF - β1 but also PGE - 2, and IL - 10.

Cells were lysed on ice using lysis buffer (10 mM Tris - HCl pH 7.4, 150 mM NaCl, 1 % Triton X-100, protease inhibitor mix G (Serva) and 2 mM Na3VO4).

Cells were lysed in RIPA lysis buffer.

However, because M. barkeri has a complex cell wall, produces a thick outer layer of polysaccharide, and grows in large aggregates of cells rather than as individual cells, standard RNA extraction protocols give incomplete lysis of cells and result in low yields of poor - quality RNA.

The reasons include: depletion of the messenger molecule nitric oxide, lysis of red blood cells and alterations in the remaining cellsâ $ ™ stiffness.

For the coimmunoprecipitation experiments, HuH - 7 cells transfected with pcDNA - SRα / FLAG - Sp 1 were harvested and cell lysates were prepared using immunoprecipitation lysis buffer [20 mmol / L Tris - Cl (pH 7.5), 150 mmol / L NaCl, 10 % glycerol, and 1 % Triton X-100].

Protein was extracted into 200 μl of Luciferase Cell Culture Lysis Reagent (Promega), and 40 μl of protein extract was used for luciferase assays.

NHLF cells transfected with siRNA molecules were lysed in High Salt ELB lysis buffer [1 M Tris pH 8.0, 1 % NP - 40, 250 mM NaCl, 5 mM EDTA] supplemented with protease and phosphatase inhibitors (1x G - Biosciences Protease Arrest, 200 µM Na3VO4, and 1 mM PMSF).

Cells were lysed with TN1 lysis buffer (50 mM Tris, 125 mM NaCl, 1 % Triton X-100, 10 mM EDTA, 10 mM sodium fluoride and 10 mM sodium pyrophosphate) supplemented with protease inhibitor cocktail (1.2 mM AEBSF, 0.46 μM aprotinin, 14 μM bestatin, 12.3 μM E-64, 112 μM leupeptin, 1.16 μM pepstatin)(Amresco; Solon, OH)-RRB- and 1 mM sodium orthovanadate (Enzo Life Sciences; Farmingdale, NY).

Make up «direct lysis buffer» by adding 1 mL of Proteinase K (e.g. Sigma P4850) to 100 mL DirectPCR Lysis Reagent (cell)(Viagen Biotech, Cat # 302 lysis buffer» by adding 1 mL of Proteinase K (e.g. Sigma P4850) to 100 mL DirectPCR Lysis Reagent (cell)(Viagen Biotech, Cat # 302 Lysis Reagent (cell)(Viagen Biotech, Cat # 302 - C).

New CFSE - based assay to determine susceptibility to lysis by cytotoxic T cells of leukemic precursor cells within a heterogeneous target cell population.

At the end of the desired treatment times, cell lysates were prepared in lysis buffer (1 % NP - 40, 0.5 mM Tris - HCl (pH 7.5), 0.14 M NaCl, 5 mM KCl, 5 mM EDTA, and 1 mM phenylmethylsulfonyl fluoride) plus serine / threonine phosphatase inhibitor cocktail.

48 hours after transfection, cells were lysed with 100 µL / well 1 × passive lysis buffer (PLB, Promega) for 15 minutes with shaking.

Cells were incubated at with 21 % O2 (lanes 1 - 6) or 1 % O2 (lanes 7 - 12) for 4h before lysis.

Each single cell was carried through two rinses of PBS using a finely drawn glass capillary pipette under a dissecting microscope and then transferred in a minimal volume (0.5 µL) to a PCR tube containing lysis buffer, protease, tRNA and poly - T gripNA ™ probe (mTRAP ™ midi - kit, Active Motif, cat.

This was found to cause lysis in hepatocellular carcinoma cell lines, but not in normal liver cells indicating that further experimental studies on SOCS3 could increase its future applicability as a cancer cell specific anti-cancer agent.59

Beta - glucanase promotes complete digestion of cereals and grains as well as lysis of yeast cell walls.

Audi spent a couple of days in the hospital on supportive care after starting chemotherapy to help avoid side effects of tumor lysis, a syndrome that can occur and make the patient very ill when a large volume of tumor cells are rapidly killed by chemotherapy.

Three mechanisms within the body cause the cells to die: lysis, ischemia, and vascular compromise.