In addition to feeder free serum and
LIF containing media, ES cells can be cultured in minimal serum free media (referred to as 2i) containing the MEK inhibitor PD0325901 that targets the phospho ERK branch of the FGF pathway and the GSK3 - β antagonist CHIR99021 [42].
Not exact matches
The NSCs population was expanded in Neurobasal media
containing 1x NEAA, 1x L - Glutamine (2 mM), 1x B27,
LIF and FGF2 20 ng / ml.
Briefly, ES cells were kept in standard ES cell medium
containing leukemia inhibitory factor (
LIF) which prevents cell differentiation.
A
LIF is a registered retirement income fund (RRIF) that
contains pension funds.