(A) Flow cytometry of two independent HV clones (HV 5.1 and HV 16.1) cultured either under self - renewing conditions or in the absence of
LIF show the presence of a subpopulation of cells positive for Venus and / or the ES cell surface marker SSEA - 1.
Not exact matches
This
shows the reaction mechanism for converting hydrogen fluoride (HF) impurity from the electrolyte into lithium fluoride (
LiF) in the solid - electrolyte interphase (SEI) with release of hydrogen gas (H2).
MIXED SIGNALS Minibrains grown in the lab form nerve cells (red) prematurely and
show signs of dying cells (green) when treated with a signaling molecule called
LIF.
Our unpublished data
show that HFF cells can support derivation and culture of mouse ES cells in an undifferentiated state without exogenous
LIF for more than 30 passages.
Treatment of cell aggregates with sodium vanadate in the presence of
LIF has been
shown to repress Nanog and stimulate PrEn differentiation [40].
In contrast, the same mouse ES cells cultured on MEF cells began to differentiate after 2 passages in the absence of
LIF (data not
shown).
Figure 2A
shows that in the presence of the cytokine
LIF, the majority of Venus - positive cells (70 %) were also SSEA - 1 positive (V+S +), while
LIF withdrawal both increased the percentage of the population expressing high levels of the Venus transgene (mean level of fluorescence increases approximately 2-fold, Figure 2A) and led to a substantial increase in a second Venus positive population that is SSEA - 1 negative (V+S −).
Likewise, it has been
shown that signaling pathways and transactivation potentials triggered by extracellular stimuli such as BMPs,
LIF, and other factors play major regulatory roles (for review see [1], [2]-RRB-.
this is one of the greatest movie i had watched in my
lif and i really apprechiate the way the actors acted in the movie and the director directed this movie... its really cool and
shows the sadness of the prision life this movie also
shows how hard the life can be 4the prisoners..