Sentences with phrase «laminin cell»

We offer defined and xeno - free laminin cell culture matrices for reliable expansion of pluripotent cell and differentiation and maintenance of specialized cell types

Our laminin cell culture matrices allow you to imitate the natural cell - matrix interaction in vitro, leading to improved cell functionality

Laminin cell culture matrices — The key to efficient derivation and reliable culture of stem cells and specialized cells lies within these extracellular matrix proteins

Our laminin cell culture matrices allow you to imitate the natural, cell - specific cell - matrix interaction in vitro, leading to improved cell functionality

BioLamina believes in sharing knowledge - here you will find information available on all the laminin cell culture applications.

Not exact matches

Next will the atheists try to remove Laminin from their body a cell shaped like a cross.

Now they report recovering not just collagen — which conveys little evolutionary information because it is the same in almost all animals — but also haemoglobin, elastin and laminin, as well as cell - like structures resembling blood and bone cells.

They discovered that all three lock onto a barrier - cell protein called the laminin receptor.

Cells were resuspended in HEPES buffer containing the following (in mm): 136 NaCl, 5.6 KCl, 1 MgCl2, 2.2 CaCl2, 11 glucose, 10 HEPES; pH adjusted to 7.4 with NaOH before plating on poly - d - lysine (0.02 mg / ml; BD Bioscience) and laminin (0.02 mg / ml; Sigma - Aldrich)- coated coverslips.

Laminin α4 (LAMA4) Expression Promotes Trophoblast Cell Invasion, Migration, and Angiogenesis and is Lowered in Pre-Eclamptic Placentas.

Stem cell culturing of human ES and iPS cells on recombinant laminin matrices is easy and efficient.

Buy human recombinant laminins from BioLamina - culture stem cells, differentiate to neurons, RPE cells, cardiomyocytes and other cells on their specific laminin isoform.

Laminin - 521 allow not only self - renewal, but also cloning, derivation, and even clonal derivation of new hES cell lines under defined and xeno - free conditions.

Laminin - 521 induce fast migration of the cells and laminin isoform specific activation of PI3K / Akt pathway through interaction with α6β1 inLaminin - 521 induce fast migration of the cells and laminin isoform specific activation of PI3K / Akt pathway through interaction with α6β1 inlaminin isoform specific activation of PI3K / Akt pathway through interaction with α6β1 integrin.

Laminin - 111 is important during early epithelial development and differentiation of the epiblast, and is commonly used as a general attachment protein in cell culture.

Here, we show hES cells plated as single cells on top of recombinant laminin - 521, growing from day 0 to day 5 when they are ready to be split again.

LAMscreen is a kit with all our currently available laminin isoforms so that you can easily screen the best laminins for different cell types.

Here, Kate presents her work on that show that culture of human ES cells on human recombinant laminin - 521 and laminin - 111 substrates significantly improve hepatocyte differentiation, maturation, function and stabilization of phenotype compared to Matrigel cultured cells.

LN521 based stem cell cultures grow as monolayers on top of the laminin substrate and remain pluripotent without spontaneous differentiation.

Human recombinant laminin isoforms are developed for defined and xeno - free stem cell culturing and primary cell cultures.

Laminin - 521 human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno - free and biologically relevant cell culture system.

Laminin cultured cells arrange themselves in lobule like structures, express MRP1 and MRP2 and are capable of biliary efflux and show significant effect on hepatocyte P450 enzyme metabolic activity.

The laminin - 521 matrix is biologically relevant and allows low density seeding of single cells without need of ROCKi.

The laminin - 521 stem cell matrix is a feeder - free, chemically defined and xeno - free cell culture system that support high cell survival and fast proliferation of hESC and iPSC.

Cells were plated either on glass coverslips (40,000 cells / coverslip) coated with poly - L lysine and laminin or six - well plates (106 cells / well) coated with laminin aCells were plated either on glass coverslips (40,000 cells / coverslip) coated with poly - L lysine and laminin or six - well plates (106 cells / well) coated with laminin acells / coverslip) coated with poly - L lysine and laminin or six - well plates (106 cells / well) coated with laminin acells / well) coated with laminin alone.

Tumor cell alpha3beta 1 integrin and vascular laminin - 5 mediate pulmonary arrest and metastasis.

Cells were grown on polyornithine and laminin coated dishes and passaged 1:2 with PBS + + (PBS with 1 % BSA).

Morphogenetic effects of soluble laminin - 5 on cultured epithelial cells and tissue explants.

The LG3 module of laminin - 5 harbors a binding site for integrin alpha3beta1 that promotes cell adhesion, spreading, and migration.

The human NSCs have a typical, undifferentiated neural stem cell morphology when expanding as monolayers on laminin - coated plates (Fig. 1A).

For example, new combinations of domains in death-fold domain proteins and laminins possibly allow for the modification of protein interactions and pathways involved in programmed cell death and cell adhesion, respectively (Supplementary Note 9.3), and the co-option of sponge -, eumetazoan - or bilaterian - specific architectures into novel functions.

Laminin - 511 is a major component of the basement membrane, which is expressed in early development of the embryo and can be used as a matrix for pluripotent (ES / iPS) stem cells, as it binds to integrin on cell surfaces.

The vaccinated Purdue dogs also developed autoantibodies to laminin, which is involved in many cellular activities including the adhesion, spreading, differentiation, proliferation and movement of cells.

Studies at Purdue University showed that canine vaccines grown in calf serum caused antibodies to be made to many calf proteins including red blood cells; thyroid; DNA; connective tissue proteins such as collagen, fibronectin, laminin, albumin, transferrin; and others.