Sentences with phrase «luc luciferase reporter»

For DNA transfection, the HepG2, 293, and 293T cells were seeded respectively at 2 × 105, 2 × 105, and 1 × 105 cells / well in 24 - well plates overnight, and then the p53 - TA - Luc luciferase reporter plasmid and RSV - lacZ plasmids were transiently transfected into the cells using NTRII for 6 h, followed by treatment with the test compounds.

Not exact matches

The p21waf1 / cip1 promoter — driven luciferase reporters (pGL3 derivatives) containing point mutations in six Sp1 elements, (Mut1) p21 - Luc, (Mut2) p21 - Luc, (Mut3) p21 - Luc, (Mut4) p21 - Luc, and (Mut5 / 6) p21 - Luc and the parental wild - type reporter, (Wt) p21 - Luc, were kindly provided by Dr. D. Kardassis (Department of Basic Sciences, University of Crete Medical School, Heraklion, Crete, Greece; ref.

The results suggested that the levels of activation induced by DDX3 of the luciferase activity of serial reporter constructs from (− 1,002 / +8) p21 - Luc to (− 123 / +8) p21 - Luc were comparable with the level of activation elicited by the full - length promoter - driven reporter p21 - Luc (5 - to 10-fold; Fig. 3B and Supplementary Fig.

p21waf1 / cip1 promoter — driven luciferase reporter (p21 - Luc; 0.05 - 0.5 μg) and increasing amount (0.1 - 2 μg) of FLAG - DDX3 expression construct were cotransfected into various cell lines as indicated.

A, schematic representation of serial p21waf1 / cip1 promoter — driven luciferase reporters used in transactivation assay: p21 - Luc contains the 2.3 - kb full - length of p21waf1 / cip1 promoter (from − 2,326 to +10 nucleotide related transcription start site); its derivatives (− 159 / +8) p21 - Luc, (− 123 / +8) p21 - Luc, (− 84 / +8) p21 - Luc, (− 76 / +8) p21 - Luc, (− 63 / +8) p21 - Luc, and (− 56 / +8) p21 - Luc contain a series of deleted promoters of p21waf1 / cip1.

The clone, pGL - MARE - Luc, was confirmed via DNA sequencing before being used in the luciferase reporter assay.