Sentences with phrase «luciferase reporter assay»

Luciferase activities were measured using the Dual - Luciferase Reporter Assay System (Promega) and normalized to Renilla luciferase activity.

The Dual - Luciferase Reporter Assay System (E1910; Promega, Madison, WI) was used to perform dual - reporter assays on NFκB luciferase and Renilla luciferase (internal control).

Luciferase activities were measured using the Dual - Luciferase Reporter Assay kit (Promega) following the manufacturer's instructions.

(a) Luciferase reporter assay of COX - 2 promoter.

The clone, pGL - MARE - Luc, was confirmed via DNA sequencing before being used in the luciferase reporter assay.

To more fully explore the effects of compound on Bach1 activity, a luciferase reporter assay using the HMOX1 E2 ARE as a target was developed.

At 48 hours, cells were washed twice with PBS and lysed in 1 × passive lysis buffer (Dual - Luciferase Reporter Assay, Promega).

After 24 hours, luciferase activities were measured by using dual - luciferase reporter assay system (Promega).

Not exact matches

TNA3: BPRC will offer access to its library of human cell reporter assays for the identification of innate immune receptor - induced signalling cascades, including human cell lines transfected with PAMPs such as TLR and / or engineered to express luciferase in response to NF - kB or IFRE - mediated signal transduction, and cell lines that provide insight into the intracellular signalling cascades of innate immune receptors.

A, schematic representation of serial p21waf1 / cip1 promoter — driven luciferase reporters used in transactivation assay: p21 - Luc contains the 2.3 - kb full - length of p21waf1 / cip1 promoter (from − 2,326 to +10 nucleotide related transcription start site); its derivatives (− 159 / +8) p21 - Luc, (− 123 / +8) p21 - Luc, (− 84 / +8) p21 - Luc, (− 76 / +8) p21 - Luc, (− 63 / +8) p21 - Luc, and (− 56 / +8) p21 - Luc contain a series of deleted promoters of p21waf1 / cip1.

The cells were cotransfected with the reporter gene constructs and related expression plasmids as indicated by using SuperFect (Qiagen) followed by transcriptional activity assay using Dual - Luciferase Assay System (Promega) according to the manufacturer's instrucassay using Dual - Luciferase Assay System (Promega) according to the manufacturer's instrucAssay System (Promega) according to the manufacturer's instruction.

(E) Luciferase assays as in (B) demonstrating the effects of mutation of Y327 to alanine or phenylalanine on indicated reporter constructs.

(E) Luciferase assay and western blot analysis of 3T3 cells following transfection with PORE reporter and overnight treatment with indicated cAMP analogues.

BPRC will offer access to its library of human cell reporter assays for the identification of innate immune receptor - induced signalling cascades, including human cell lines transfected with PAMPs such as TLR and / or engineered to express luciferase in response to NF - kB or IFRE - mediated signal transduction, and cell lines that provide insight into the intracellular signalling cascades of innate immune receptors.