Sentences with phrase «m ethylenediaminetetraacetic»

Not exact matches

Cells were collected from either the media (floating subpopulation) or the adherent subpopulation after each hypoxic cycle and dissociated into single - cell suspension by trypsin / EDTA (trypsin / ethylenediaminetetraacetic acid).

Cells were lysed 48 h later with modified radioimmunoprecipitation assay buffer (RIPA buffer, 50 mM Tris - HCl, pH7.4, 150 mM NaCl, 1 % Nonidet P - 40 (NP - 40), 0.25 % sodium deoxycholic acid, 1 mM ethylenediaminetetraacetic acid (EDTA), 1 mM phenylmethylsulphonyl fluoride (PMSF), 1X Complete cocktail protease inhibitor (Roche, Penzberg, Germany)-RRB-.

In the case of heavy metals such as mercury, there is a locking mechanism that is difficult to overcome with foods or herbs and chelators (compounds that combine with metals) such as Dimercaptosuccinic Acid (DMSA) or Ethylenediaminetetraacetic acid (EDTA) may need to be used.

Ethylenediaminetetraacetic acid (EDTA) preserves cellular components for CBC and morphologic evaluation but falsely decreases calcium, phosphorus, and alkaline phosphatase levels and elevates potassium levels; therefore, EDTA plasma samples should not be used for chemical analysis.