Sentences with phrase «mcf7 breast cancer cell lines»

The researchers observed the effect of the synthetically produced molecule, JK - 31, on the growth and proliferation of a model human breast cancer cell line and found that it effectively blocked the protein cyclin - dependent kinase 1 (CDK1), which plays a key part in the process of the division of cancer cells, and therefore inhibited the proliferation of the cells.

Overexpression of ZMYND11 in an osteosarcoma cell line and a triple - negative breast cancer cell line inhibited tumor growth.

To see whether cancer stem cell renewal involves a chain of events similar to that used by embryonic stem cells, and whether the process was affected by oxygen levels, Semenza and graduate student Chuanzhao Zhang focused their studies on two human breast cancer cell lines that responded to low oxygen by ramping up production of the protein ALKBH5, which removes methyl groups from mRNAs.

Banik cautions that neither of the contrast agents has yet been tested on animals injected with breast cancer cell lines, which he calls an important step to take.

With help from researchers at Duke University, they showed that the gene has a unique pattern of methylation in breast cancer cell lines that might one day help doctors spot the disease.

Ana Soto, professor of cellular biology at Tufts University School of Medicine, and her colleagues were studying the effects of estrogen on a breast cancer cell line.

«Both the natural and the synthetic substances inhibit the growth and spread of cancer stem cells in breast cancer cell lines.

The researchers have isolated the sesquiterpene lactone damsin from the plant and studied its effect on cancer stem cells in three different breast cancer cell lines.

He is researching the functions and properties of human sulfotransferase (EST) enzymes in human breast cancer cell lines.

Recent studies with human breast cancer cell lines have also shown that Shp2 mediates survival signals in cancer cells.

We purchased MDA - MB - 231 (MB231) and MCF7 breast cancer cell lines from American Type Culture Collection.

They collected gene expression data from six genetically diverse breast cancer cell lines, which were each exposed to 109 small - molecule drugs at multiple dosages and time points.

The example shown reveals that these estrogen agonists show the strongest connectivity to each other in MCF7, a human breast cancer cell line that expresses the estrogen receptor.

AAV2 mediated cell killing of multiple breast cancer cell lines representing both low and high grades of cancer and targeted the cancer cells independent of hormone or growth factor classification.

al. 47, the expression profiling of ABC transporters in a drug - resistant breast cancer cell line was investigated using AmpArray.

The researchers then injected AAV2 into human breast cancer cell line - derived tumors in mice without functioning immune systems.

Quantitative proteomics study of breast cancer cell lines isolated from a single patient: discovery of TIMM17A as a marker for breast cancer.

Here, we show that a non-adherent, stem - like, and metastatic CSC - enriched subpopulation could be isolated by exposing human metastatic breast cancer cell lines to cycles of chronic hypoxia followed by reoxygenation.

In our study, we found that exposing metastatic breast cancer cell lines to hypoxia and reoxygenation cycles induces a unique subpopulation that is highly metastatic and exhibits stem - like and EMT phenotypes.

These results provide the validity to the newly developed hypoxia / reoxygenation culture system for examining the regulation of CSCs in breast cancer cell lines by niche factors in the tumor microenvironment and developing differential targeting strategies to eradicate breast CSCs.

Using a qRT - PCR - based method, we found dramatic decreases of epithelial miRNAs (miR200c and miR205) in the F3 subpopulations compared with the parental breast cancer cell lines (Figure 6e).

Using cutting - edge techniques enabled by next - generation sequencing, the authors generated complete methylome maps at single nucleotide resolution in a low - passage breast cancer cell line and normal breast tissue (primary human mammary epithelial cells).

We then isolated and exposed this novel subpopulation to additional cycles of hypoxia / reoxygenation and established a distinct subpopulation of cells from these two breast cancer cell lines.

To study the effect of hypoxia / reoxygenation cycles on breast cancer, we exposed two metastatic human breast cancer cell lines (MDA - MB 231 and BCM2) to cycles of chronic hypoxia and nutrient deprivation.

Ab7291 staining alpha tubulin in human breast cancer cell line by ICC / IF (Immunocytochemistry / Immunofluorescence).

Conditions for hypoxia / reoxygenation cycles were optimized using two human metastatic breast cancer cell lines (MDA - MB 231 and BCM2).

Breast Cancer Cell Line Classification and Its Relevance with Breast Tumor Subtyping Xiaofeng Dai, Hongye Cheng, Zhonghu Bai, Jia Li Journal of Cancer 2017; 8:3131 - 3141

When Possemato suppressed PHGDH protein production in breast cancer cell lines with elevated levels of it, the cells stopped proliferating.

The human breast cancer cell line SKBR3 was purchased from the Duke University Cell Culture Facility and was maintained in McCoy's 5A medium (Life Technologies) supplemented with 10 % FBS (Life Technologies) and antibiotics.

To directly evaluate the relationship between ABL family kinases and metastasis, we analyzed ABL1 and ABL2 protein abundance in MDA - MB - 231 — derived breast cancer cell lines with different organ metastasis tropisms (19).

Breast cancer cell lines contain functional cancer stem cells with metastatic capacity and a distinct molecular signature.

The effect of a novel antagonist of growth hormone releasing hormone on cell proliferation and on the key cell signaling pathways in nine different breast cancer cell lines.

Cultures of the human triple negative breast cancer cell line, HCC1806, were maintained in RPMI 1640 medium supplemented with 10 % FBS.

Importantly, each supernatant labeled MCF7 cells between one and two logs less than either of the HER2 - overexpressing breast cancer cell lines.

In collaboration with Nanyang Technological University, experiments were also conducted to co-deliver paclitaxel and small interfering RNA (siRNA) targeting a protein that prevents cell death (Bcl - 2) to MDA - MB - 231 human breast cancer cell line.

Recent reports show increased CSC activity following hypoxic exposure in breast cancer cell lines (18 — 20), but no reports have studied this rare population in primary human breast cancer samples.

To confirm specificity, supernatant from transfected or transduced HB1.F3 cells was incubated with MCF7, MCF7 / HER2, or BT474, a human breast cancer cell line that endogenously overexpresses HER2.

To assess the ability of F3 - IgG to inhibit the proliferation of HER2 - overexpressing breast carcinoma cells, we measured the proliferation of BT474, MCF7 / HER2 and MCF7 breast cancer cell lines in the presence of trastuzumab, F3 - IgG, or an isotype - matched control antibody.

It can efficiently introduce DNA into a cell to be incorporated into its genetic make - up, i.e. induce high gene expression level, especially in both human and mouse breast cancer cell lines, and mouse breast cancer model.

Earlier reports support the observed hypoxia induced increase in CSC in breast cancer cell lines (18 — 20), but the ER - α — dependent contrasting reaction has not been reported elsewhere.

Both reviewers consider that since the functional studies were only tested in 2 basal and 2 luminal breast cancer cell lines, the sample set was too small, and testing additional cell lines for their dependency on MELK should further strengthen the authors conclusions.

Consistent with the demonstration that LARP7 is necessary for the integrity of the 7SK snRNA (He et al., 2008), the reduced LARP7 expression in invasive breast cancer cell lines was accompanied by a decrease in 7SK snRNA (Figure 1D).

LARP7 was expressed at relatively high levels in the untransformed mammary epithelial cell lines (MCF10A and EpH4) and noninvasive breast cancer cell lines (MCF7, BT474, T74D, and ZR75B), but was markedly reduced in all four invasive and metastatic cancer lines examined (MDA - MB - 468, BT549, MDA - MB - 231, and MDA - MB - 435; Figure 1D).

The functional data provided from a limited series of breast cancer cell lines does not allow generalizable conclusions to be drawn.

Dissertation: «Epigenetic Modification of Vitamin D - induced Gene Expression in Human Colorectal and Breast Cancer Cell Lines.»

The omega - 3 metabolites also reduced the ability to move in the triple - negative breast cancer cell lines by 20 to 60 %.

The omega - 3s were effective against all kinds of cells that were cancerous, but the effect was seen to be more pronounced in triple - negative breast cancer cell lines, with a proliferation reduction of up to 90 %.

The curcumin metabolite tetrahydrocurcumin decreases the activity of the phase III transporters in human cervical carcinoma and breast cancer cell lines.

One study examined the cytotoxic effects of different types of clove extract on breast cancer cell lines, including water - based, alcohol - based and essential oil extracts.

Not exact matches

The research, conducted in cell lines and mouse models, explored enhancing the cancer - killing effects of PARP inhibitors not only in regard to AML but also triple - negative breast cancer.