Sentences with phrase «mcf7 xenografts»

«This study is important, in part because we now have a new xenograft model that will for the first time allow us to apply new biomarkers to better predict disease progression and test new therapeutics.»

To unravel exactly how ADAR1 is connected to disease severity at a molecular level, the researchers transferred multiple myeloma patient tissue to mice, creating what's known as a xenograft or «humanized» model.

They also found that silencing the ADAR1 gene in the xenograft model reduced multiple myeloma regeneration.

A decade ago, he replicated the entire human leukemia disease process by introducing oncogenes into normal human blood cells, transplanting them into xenografts (special immune - deficient mice that accept human grafts) and watching leukemia develop — a motherlode discovery that has guided leukemia research ever since.

«The ongoing line of work with this drug is an excellent example of the bench getting even closer to the bedside — our lab work with the drug in patient - derived xenograft models of disease makes possible the clinical trials taking place at the University of Colorado Hospital next door,» Jimeno says.

Finally, they were also able to reduce tumor growth in their first in vivo xenograft analysis.

Additional experiments also showed that TiY can suppress the tumor growth in mice xenograft model.

Through these effects, the PERY peptide reduced the proliferation of several (but not all) cancer cell lines in culture and inhibited the growth of a human breast cancer xenograft in mice.

To overcome these problems, Min and his team developed a new modality to visualize glucose uptake activity inside single cells based on stimulated Raman scattering (SRS) imaging, and demonstrated its use in live cancer cells, tumor xenograft tissues, primary neurons and mouse brain tissues.

«Our data confirmed that, while the rate of growth of triple - negative breast cancer was not affected by CDK 4/6 inhibitors, this class of drugs was able to significantly inhibit the spread of triple - negative breast cancer to distant organs when tested in multiple different triple - negative breast cancer models, including patient - derived xenografts.»

Terbinafine, a U.S. Food and Drug Administration — approved antifungal drug targeting SQLE, markedly inhibited SQLE - induced NAFLD - HCC cell growth in NAFLD - HCC and HCC cells and attenuated tumor development in xenograft models and in Sqle transgenic mice.

EZH2 inhibition delayed tumor onset in KDM6A - null cells and caused regression of KDM6A - null bladder tumors in both patient - derived and cell line xenograft models.

We created a mouse xenograft model in which SiHa cervical cancer cells were injected into mice subcutaneously, and the resultant tumors were treated with PAL three times a week for 6 weeks.

This research was also supported by the Athymic Animal and Xenograft Core Facility and the Cytometry & Imaging Microscopy Core Facility of the Case Comprehensive Cancer Center (P30CA043703).

We then usually move onto xenograft models and then also, if available, try to test some of these compounds in genetically engineered mouse models that have particular mutations driving tumor formation.

In his current position at The Jackson Laboratory, he has overall responsibility for the ongoing operational development of the PDX resource, which generates, banks, and distributes patient - derived xenograft (PDX) mouse models of human cancers.

The researchers, including scientists from The Genome Institute at Washington University School of Medicine, presented the research titled, «Patient - derived xenograft study reveals endocrine therapy resistance of ER + breast cancer caused by distinct ESR1 gene aberrations.»

He developed new techniques for transplant surgery, helped to make kidney transplantation viable and was one of the first researchers to try xenografts — in the 1960s he placed baboon kidneys in six patients.

In these experiments, Berger and colleagues show that somatic PREX2 mutations identified through whole - genome sequencing of human melanoma can contribute to enhanced lethality of tumor xenografts in nude mice (Figure 3B, S6B, and S6C; Berger et al., 2012).

We thank the Penn Genome Frontiers Institute and the University of Pennsylvania's Stem Cell and Xenograft Core and DNA Sequencing Facility for 454 sequencing.

Their paper, titled «Monitoring oxygen levels in orthotopic human glioma xenograft following carbogen inhalation and chemotherapy by implantable resonator - based oximetry,» was published recently in the International Journal of Cancer.

First, a single xenograft mouse is not enough.

In a 1988 paper summarizing his findings, Fiebig concluded that xenograft mice were wonderful models for broadly testing new drugs against human tumors, but they «can not be used as a clinical routine method» for predicting patient treatment.1 The idea of using xenograft mice as personal avatars for cancer patients was discarded.

Once seeded onto a proper support, these keratinocytes appeared capable of forming a pluristratified epidermis both in vitro and in vivo as xenografts in immunodeficient mice.

The researchers used an in vivo model of PARP inhibitor resistance in patient - derived tumor xenografts (PDXs).

Personal drug regimens based on xenograft mice harboring a single patient's tumor still need to prove their true utility in medicine.

«There is some talk of going into 3 - D cell models rather than xenografts, because it is so difficult and challenging to grow [the transplanted tumors],» says Manish Kohli, the Mayo Clinic oncologist leading the PROMOTE study.

Using 80 of the successful tumor xenografts — from the Greek «xenos» meaning «foreign» — he compared how the mouse's tumor responded to a drug or drug combination with the treatment response of the corresponding human patient.

Dynamic clonal progression in xenografts of acute lymphoblastic leukemia with intrachromosomal amplification of chromosome 21

AMSBIO announces the availability of two new formulations of Basement Membrane Extract (BME) known as Cultrex BME 2 (organoid growth matrix) and Cultrex BME 3 (xenograft / tumorgraft matrix).

Moreover, following transformation with H - Ras, these cells initiated tumor xenografts in immunocompromised mice with higher efficiency than their epithelial counterparts [5].

These days, mice grafted with human tumors, known as patient - derived xenograft (PDX) mice, are common in cancer research laboratories.

Therapeutic efficacy of aldoxorubicin in an intracranial xenograft mouse model of human glioblastoma.

NCI's efforts to develop new laboratory models of human cancer includes vastly increasing the number of human cancer cell lines (grown as two - dimensional and three - dimensional cultures) and patient - derived tumor xenografts.

Over 300 patient - derived xenograft (PDX) tumors are available for engrafting into immunocompromised NSG ™ mice for shipment to your facility...

Of 21 patients who responded to a particular drug or drug combination, their xenograft mice correctly predicted the outcome 90 percent of the time.

The xenografts often grew too slowly to provide timely results; more than half of the 80 patients needed treatment before their mice yielded actionable data.

Due to the high efficiency of establishing organoid models from different tissues and diseases, such as cancer, organoid technology allows the generation of large living biobanks of tumor organoids that are amenable for middle - throughput drug screens and may allow personalized therapy design, as a complement to cell line and xenograft - based drug studies (7,19).

XIAP downregulation accompanies mebendazole growth inhibition in melanoma xenografts.

Reconstitution with syngeneic plus allogeneic or xenogeneic bone marrow leads to specific acceptance of allografts or xenografts.

Anti-LFA-1 improves pig islet xenograft function in diabetic mice when long - term acceptance is induced by CTLA4Ig / anti-CD 40L.

Influence of gemcitabine chemotherapy on the microbiota of pancreatic cancer xenografted mice.

Since starting her new role as an investigator in the In Vivo Pharmacology group at Novartis Oncology, Bhang has been working on using the ClonTracer library to monitor clonal dynamics following drug treatment in cell line and primary tumor xenograft models.

Resources include extensive genomic and drug sensitivity data sets, orthotopic xenografts and preclinical models.

Using a zebrafish embryo xenograft model we also demonstrated that anti-CD90 monoclonal antibodies decreased the viability and metastatic potential of insulinoma cells, suggesting that anti-CD90 monoclonals form a potential novel adjutant therapeutic modality.

In 2012 she joined the laboratory of Dr. D.L. Konotyiannis at BSRC AL.Fleming, Greece as postdoctoral scientist, where she worked on the study of the RNA Binding Protein HuR during intestinal and lung inflammation and cancer, using inducible, KO and Tg mouse models and xenografts.

Zebrafish larva implanted with cancer patient's tumor (red) RITA FIOR / CHAMPALIMAUD CENTRE FOR THE UNKNOWNSee R. Fior et al., «Single - cell functional and chemosensitive profiling of combinatorial colorectal therapy in zebrafish xenografts,» PNAS, doi: 10.1073 / pnas.1618389114, 2017

The ClonTracer library originally came about as Bhang and co-workers were trying to develop a way to quantify the take rate of mouse xenografts after cell implantation.

A physicist turned computational scientist, Graber comes to the MDI Biological Laboratory from The Jackson Laboratory, also located in Bar Harbor, where he focused on computational approaches to understanding post-transcriptional gene regulation and interactions, while also working intensively to process, analyze and interpret the genome - scale data sets generated within the Patient Derived Xenograft (PDX) cancer study program.

Most xenograft models for brain tumors using tumor cell lines require at least 10,000 cells.