Sentences with phrase «many of the endothelial cell»

Dismantling structural filaments within the cytoskeletons — the internal skeletons — of the endothelial cells creates the large holes, which can be several microns in diameter.

Sydenham chorea was like rheumatic fever of the brain, thought to occur when rheumatic fever progresses and strep antibodies (emboldened by fever) breaches the blood - brain barrier (BBB), the tight wall of endothelial cells ordinarily there to protect the brain from the outside world.

In a collaborative effort between the Gladstone laboratories of Benoit Bruneau, PhD, Katherine Pollard, PhD, and Dr. Srivastava, the scientists used stem cell technology to make large amounts of endothelial cells from patients with CAVD, comparing them to healthy cells and mapping their genetic and epigenetic changes as they developed into valve cells.

In contrast, lymphocytes in both types of endothelial cell grafts expressed higher levels of genes known to be involved in dampening the immune response and inducing self - tolerance.

They found that, while the grafts of undifferentiated iPS cells harbored large numbers of T cells of only a few specificities — indicating a robust immune response — those found in grafts of the two types of endothelial cells were more diverse, suggesting a more limited response associated with a phenomenon known as self - tolerance.

To explore this idea, they induced heart attacks in mice and then studied the fibroblasts to see if the cells expressed markers characteristic of endothelial cells.

A byproduct of HO - 1 is carbon monoxide, which promotes the survival of endothelial cells.

The Max Planck researchers then showed that less necroptosis of endothelial cells and less metastasis occur in genetically modified animals in which Death Receptor 6 is disabled.

More of the antibodies that coat the nanorod can therefore bind receptors on the surface of endothelial cells, and that leads to more effective cell adhesion and more effective drug delivery.

In contrast, the elongated nanorods have a larger surface area that is in contact with the surface of the endothelial cells.

The study, published in Proceedings of the National Academy of Sciences, found that rod - shaped nanoparticles — or nanorods — as opposed to spherical nanoparticles, appear to adhere more effectively to the surface of endothelial cells that line the inside of blood vessels.

It both promotes angiogenesis within the tumor and increased the migration of endothelial cells, which form the inner lining of blood vessels.

Co-culture of endothelial cells (green) and pericytes (red) results in spheroids which are used to study the interactions of the two cell types in 3D.

An impermeable shield of endothelial cells that protects our brains from toxins and other threats that may lurk in circulating blood, the barrier can also exclude therapeutic drugs and, at times, essential biomolecules required for healthy brain development.

Yet, more recent discoveries suggest a much more active role of endothelial cells during tumor growth than has been anticipated so far.

It took advantage of the brain's own mechanism for getting a necessary nutrient, iron, across the lining of endothelial cells that form the blood - brain barrier.

One of the major impediments to obtaining a large number of endothelial cells from different tissues has been the inability to purify and propagate these cells in culture.

The ability of the endothelial cells to form capillary - like structures was assessed by placing them on the solubilized basement membrane preparation Matrigel (Becton Dickinson, San Jose, CA), according to the manufacturer's instructions.

The constitutive and inducible expression of the endothelial cell adhesion molecules E-selectin, ICAM - 1, and VCAM - 1 was assessed by immunohistochemistry (Figs. 4, A — C) ⇓.

The constitutive expression of these endothelial cell adhesion molecules was very similar for most of the lines derived from different organs, i.e., very low expression levels of E-selectin and VCAM - 1 and more pronounced expression of ICAM - 1.

Each of the endothelial cell lines established from the H - 2Kb - tsA58 transgenic mice was capable of generating vascular - like channels on Matrigel within 12 h.

This is the first evidence that starvation of endothelial cells could offer new therapeutic opportunities for the treatment of excessive angiogenesis in diseases (like cancer).

We have observed on several occasions that, for the majority of endothelial cell lines, the transfer to 37 °C conditions for 72 h correlated with a 50 % reduction in cell proliferation, a value that continued to decrease with time.

As expected, all of the endothelial cell lines showed diminished constitutive expression of E-selectin (data not shown) and VCAM - 1 (Fig. 4C) ⇓ in comparison with surface levels of ICAM - 1 (Fig. 4B) ⇓.

Established cell lines exhibited several inherent endothelial properties, including the expression of constitutive and inducible levels of endothelial cell adhesion molecules E-selectin, intercellular adhesion molecule - 1, and vascular cell adhesion molecule - 1, internalization of acetylated low - density lipoprotein, and formation of loop structures on Matrigel surfaces.

To determine inducible endothelial cell adhesion molecule expression (E-selectin, VCAM - 1, and ICAM - 1), some of the chambers were given medium containing 10 ng / ml TNF - α for 4 — 6 h (these time points were selected to correlate with the known kinetics of endothelial cell adhesion molecule expression; Refs.

Each of the endothelial cell lines demonstrated an ability to orient into capillary - like structures when placed onto a Matrigel surface (Fig. 5A) ⇓.

The transfer of endothelial cells to nonpermissive temperatures (37 °C) resulted in cell differentiation and the induction of a quiescent state.

Activation of endothelial cells elicits a complex series of responses that include the elaboration of proteolytic enzymes, migration, and proliferation.

Many of the endothelial cell lines described here have been considered previously inaccessible for study of tumor angiogenesis, progression, and metastasis.

Efforts to study the molecular mechanisms that mediate these complex processes in different anatomical compartments have been impeded by difficulties in the isolation and propagation of endothelial cells from different organs.

It was at this stage that highly purified populations of endothelial cells could be obtained by adjusting the gating parameters to select only the most intensely labeled cells.

Efforts to promote a selective increase in the number of endothelial cells present in primary cultures by using different media preparations (MCDB - 131, D - Valine, etc.) were largely unsuccessful.

By having such a mechanism, it is possible to minimize the loss of endothelial cells over a lifetime, due to naturally occurring cell death.

MicroRNA - 24 inhibits the proliferation and migration of endothelial cells in patients with atherosclerosis by targeting importin - a3 and regulating inflammatory responses.

A new study describes an improved protocol for the efficient differentiation of endothelial cells from pluripotent stem cell sources and highlights the importance of the MAPK and PI3K signaling pathways

The adaptor CRADD / RAIDD controls activation of endothelial cells by proinflammatory stimuli.

More recently, we have started to investigate the role of arachidonic acid P450 epoxygenases in the control of endothelial cell function in vitro and tumorigenesis in vivo.

In vivo imaging of endothelial cell adhesion molecule expression after radiosurgery in an animal model of arteriovenous malformation.

In the past 10 years our laboratory has investigated the role of cell - matrix interaction in the control of endothelial cell functions in vivo and in vitro and provide the first evidence that the collagen binding integrin alpha1beta1 is pro-angiogenic and pro-tumorigenic and its inhibition and / or down - regulation is beneficial in the setting of tumor associated angiogenesis.

Isolation of Endothelial Cells and Vascular Smooth Muscle Cells from Internal Mammary Artery Tissue.

This gave a mixed population of cells with 20 % expressing CD34, a hematopoietic stem cell marker, with some co-expression of endothelial cell markers (CD31, Flk1, and VE - cadherin).

A vascular injury model using focal heat - induced activation of endothelial cells.

The anti-inflammatory actions of platelet endothelial cell adhesion molecule - 1 do not involve regulation of endothelial cell NF - $ ąppa $ B.

VEGF normally promotes the growth of endothelial cells, which in turn helps build new blood vessels in tumors.

The most potent of these, known as effector memory T cells, are activated by a group of proteins known as human leukocyte antigens (HLAs) on the surface of endothelial cells lining the donated organ's blood vessels.

A microscopic image of endothelial cells treated with drug - loaded nanoparticles.

The IL -1-like cytokine IL - 33 is constitutively expressed in the nucleus of endothelial cells and epithelial cells in vivo: a novel «alarmin»?

Migration of endothelial cells is required for angiogenesis to proceed.

Therefore, we studied the effects of kahweol on the growth of endothelial cells.