Sentences with phrase «mass cytometry»

As Fluidigm IP counsel she supports the mass cytometry and microfluidics technologies.

ICS analysis, mass cytometry analysis, in vivo imaging, methodology and statistical consultancy, flow and mass cytometry courses, in vivo imaging courses.

When used with mass cytometry, PLAYR allowed for the simultaneous quantification of more than 40 different mRNAs and proteins.

It creates customisable and high - scale mouse models of human disease or displaying an abnormality (KI, KO mouse models) and it provides a complete phenotyping characterisation of the immune system by polychromatic and mass cytometry measuring more than 50 simultaneous quantitative parameters in standardised conditions.

PHENOMIN - CIPHE, on the strength of its expertise in genetics and mouse immunology, is positioned as a pioneer in multiparametric analysis of the immune system of mutant mice in normal conditions or exposed to risks of infection, by an integration of innovative research and technical knowledge in the field of flow and mass cytometry as well as advanced microscopy.

For these reasons, mass cytometry has emerged as the leading technology for high - content single - cell analysis in health and disease.

Complete phenotyping of the mouse immune system by polychromatic and mass cytometry (CYTOF), thanks to a set of standardised protocols enabling isolation of viable cells from lymphoid and non-lymphoid organs (lung, skin, intestine,...) for labelling using complex ranges of antibodies whose compatibility allows the simultaneous registration of 50 quantitative parameters at least (size, structure, specific antibodies and cell viability).

The advantages of mass cytometry over traditional flow cytometry include the absence of background and that there is no need to correct for the spectral overlap of fluorophore emissions.

The current study involved monitoring the patients for one year after completed stem cell transplantation and used mass cytometry to study the different cell types of the immune system and the so called ProSeek method for simultaneous protein analysis.

We are using CyTOF mass cytometry and single cell RNA - Sequencing to identify new monocyte subsets that may be important in disease.

While mass cytometry offers a powerful and highly multiplexed method for profiling single cells, there are various technical considerations for researchers wanting to perform a mass cytometry assay for the first time, or at a more advanced level.

The scientists used diffusion maps — a basic way to order snapshots of single cells along their developmental journey — but they plan to study developmental trajectories using a new algorithm called Wishbone, which uses single - cell RNA sequencing and mass cytometry data to specifically model branching steps during cell differentiation (Nat Biotechnol, 34:637 - 45, 2016).

IDMIT is an infrastructure for preclinical research in infectious diseases and immunology which is certified ISO9001 and which includes 1) A large animal facility with capacity to host NHP in BSL2 and BSL3 containment, 2) State - of - the - art laboratories for cell biology, immunology, molecular biology, flow cytometry and mass cytometry (CyTof), cell - sorting and confocal microscopy in BSL3 containment; 3) A biological resources centre with high storage capacity; 4) Highly innovative technologies for in vivo imaging of large animals in BSL2 and BSL3 containment, including a two - photon microscope, a PET - CT facility, and several optic based technologies (fibered endo - microscopy, near infra - red imaging).

IDMIT will contribute 1) To the development and validation of assays based on flow cytometry and mass cytometry for the evaluation of immune responses in humans and animal models; these tools will be particularly relevant for the identification of signatures of vaccine efficacy; 2) To the animal model platform, in particularly by providing access to NHP models and to new technologies for in vivo imaging infections and host responses; 3) To networking activities, in particular by organising a workshop on in vivo imaging.

Not exact matches

SciLifeLab's facility for mass - cytometry (CyTOF) is now part of the national infrastructure and thus available for applications by the entire Swedish research community.

The two nodes perform standardized mass - cytometry experiments on customer provided samples using validated panels of metal - coupled antibodies.

SciLifeLab's facility for mass - cytometry (CyTOF) is now part of the national infrastructure and therefor available for applications by the entire Swedish research community.

We make use of mass - cytometry to perform multi-dimensional analysis of up to 40 - 45 parameters in millions of single cells.

The institute facilities grouped into the CurieCoreTech include advanced imaging, high throughput sequencing, bioinformatics, reverse phase protein array, proteomics and mass spectrometry, antibody technologies, cytometry, animal housing, and a unique experimental radiotherapy platform (https://science.institutcurie.org/platforms).

Practical training on multi-parameter flow, mass and spectral cytometry (respectively MFC, MMC and MSC) will be performed on one single site at CIPHE (Marseille).

To define the phenotype and function of specific cellular populations involved in the cross-talk between cancer and immune cells we make use of both flow - cytometry and mass - cytometry.

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Laboratory: Experience with laboratory applications in parasitology, immunology and molecular biology including, microscopy, BCA Protein Assay, ELISA, Flow Cytometry, Real Time qPCR, Luminex, Gas chromatography (GC) mass spectrometry, cotinine assays (saliva), magnetic bead cell separation (MACS, Percoll and Ficoll) and whole blood cultures.