Sentences with phrase «maxpab rabbit polyclonal antibody»

Membranes were probed with the following primary antibodies diluted in TBS - T with 5 % NFM overnight at 4 °C: calsequestrin rabbit polyclonal antibody (1:2,000 dilution, ab3516, Abcam), PPARδ rabbit polyclonal antibody (2 μg • ml — 1 dilution, ab23673, Abcam), COX polypeptide Va mouse monoclonal antibody (1:1,000 dilution, MS409, MitoSciences), phosphorylated Smad2 (1:1,000 dilution, 3101, Cell Signaling Technology), Smad2 / 3 (1:1,000 dilution, 3102, Cell Signaling Technology), GAPDH rabbit polyclonal antibody (1:1,000 dilution, ab9485, Abcam).

In the middle, ab18723, this is a rabbit polyclonal antibody to Doublecortin, in this image staining adult mouse denate gyrus sections.

Rabbit polyclonal antibodies to total AKT (Santa Cruz Biotechnology), rabbit polyclonal antibodies to Ser2448p - mTOR, p70S6K, Ser240 / Ser244p - rpS 6, Thr70p - 4E - BP1, total 4E - BP1, and total eIF4E (Cell Signaling Technology), mouse monoclonal antibodies to Ser473p - AKT and Thr389p - p70S6K (Cell Signaling Technology), and rabbit monoclonal antibodies to rpS6 (Cell Signaling Technology) were used.

Rabbit polyclonal antibodies to total AKT (Santa Cruz Biotechnology, Santa Cruz, CA); mTOR, Ser2448p - mTOR, Ser240 / Ser244p - rpS 6, Thr70p - 4E - BP1, total 4E - BP1, and total eIF4E (Cell Signaling Technology, Beverly, MA); and mouse monoclonal antibodies to Ser473p - AKT and Thr389p - p70S6K (Cell Signaling Technology) were used.

LGALS3 MaxPab rabbit polyclonal antibody.

Not exact matches

Bethyl's portfolio consists of polyclonal and monoclonal antibodies, including new recombinant rabbit monoclonal antibodies.

Before moving into Abcam's product offering, I would like to briefly talk about rabbit monoclonal antibodies and their advantages over a traditional mouse monoclonal or polyclonal antibodies.

Hematopoietic malignancies were diagnosed using the following markers: for B - and T - lymphocyte detection, rat antimouse CD45R / B220 mAb (Southern Biotechnology Associates, Birmingham, AL; dilution 1:100); for T - lymphocyte detection, rabbit antihuman CD3 polyclonal antibody (Dako, Glostrup, Denmark; 1:100); and for monocyte / macrophage detection, rat antimouse F4 / 80 mAb (BMA Biomedicals AG, Augst, Switzerland; 1:20) and rat antimouse CD11b mAb (Chemicon International, Temecula, CA; 1:20).

Is there any difference between the specificity of the Rabbit Anti-CD3 Monoclonal Antibody, Unconjugated, Clone SP7 cat # ab16669 and Rabbit Anti-CD3 epsilon Polyclonal Antibody, Unconjugated cat #ab49943?

Following fixation in 4 % PFA, the cells were assessed for Sall4 expression using 1:100 dilution of the primary antibody (ab29112) in 1 % serum, 0.1 % triton, 0.1 % BSA in PBS, followed by detection using goat polyclonal rabbit IgG Alexa 488 (ab150077) at 1:200.

Mesenchymal tumors were diagnosed using goat antihuman vimentin polyclonal antibody (Sigma Chemical Co., St. Louis, MO; 1:40), rabbit antichicken desmin polyclonal antibody (Sigma Chemical Co.; 1:100), and rabbit antihuman factor VIII polyclonal antibody (Dako; 1:200).

Secondary antibody 2: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor ® 594) pre-adsorbed (ab150084), 1:300 Nuclei were counterstained wantibody 2: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor ® 594) pre-adsorbed (ab150084), 1:300 Nuclei were counterstained wAntibody to Rabbit IgG - H&L (Alexa Fluor ® 594) pre-adsorbed (ab150084), 1:300 Nuclei were counterstained with DAPI

Primary antibody 2: Rabbit anti-laminin, 1:400 Secondary antibody 1: Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor ® 488) pre-adsorbed (ab150117antibody 2: Rabbit anti-laminin, 1:400 Secondary antibody 1: Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor ® 488) pre-adsorbed (ab150117antibody 1: Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor ® 488) pre-adsorbed (ab150117Antibody to Mouse IgG - H&L (Alexa Fluor ® 488) pre-adsorbed (ab150117), 1:200

After incubation with 3 % hydrogen peroxide for 10 minutes and 0.2 % horse serum for 20 minutes, a 1:200 diluted rabbit anti-DDX3 polyclonal antibody was applied for 1 hour at room temperature and followed by incubation with biotinylated linker and streptavidin - horseradish peroxidase (LSAB2 system - HRP, DAKO Cytomation, Carpinteria, CA) for 30 and 10 minutes, respectively.

After blocking for 1 hour with 10 % normal goat serum (NGS), the slides were incubated with 200 μl primary antibody, either a polyclonal rabbit anti-Borrelia (Accurate chemicals, Westbury, NY) at 1:200 or the anti-OspA monoclonal, CB10, with a 1:30 dilution of hybridoma supernatant for 1 hour at room temperature (RT).

These reagents included goat polyclonal antisera to MuLV (whole virus and gp69 / 71Env, respectively) available at ATCC (VR - 1537 and VR - 1521, respectively), and a rabbit anti-XMRV polyclonal antiserum (kindly provided by Ila Singh) and a rat anti-SFFV (7C10) monoclonal antibody (kindly provided by Sandra Ruscetti) used previously to detect XMRV protein expression and antibodies in prostate cancer and CFS patients, respectively [11, 13, 21].

Sections were then incubated overnight in primary TH antibody (PelFreez # P40101, rabbit polyclonal, 1:2000 dilution) at 4 °C.

We are grateful to Dr. Robert Silverman at the Cleveland Clinic for the VP62 XMRV plasmid, Dr. Ila Singh at the University of Utah for the rabbit anti-XMRV polyclonal sera, and Dr. Sandra Ruscetti at the National Cancer Institute for the rat anti-SFFV (7C10) monoclonal antibody.

Sections were then incubated overnight in primary TH antibody (PelFreez # P40101, rabbit polyclonal, 1:2000 dilution) and GFAP antibody (BD Pharmingen # 556329, mouse monoclonal, 1:1000 dilution) at 4 °C.

Membranes were then incubated with the same blocking solution containing rabbit polyclonal primary antibodies against HIF - 2α (1:500, ab199).

For colocalization of both tracers or one tracer together with ZENK signals, primary antibodies (Egr - 1 / ZENK, 1 ∶ 500; CtB, 1 ∶ 300 in PBS - T) were detected by an appropriate secondary antibody (polyclonal goat raised against rabbit IgG labeled with fluorescent dyes Alexa488; Molecular Probes Europe BV, Leiden, The Netherlands, 1 ∶ 400 in PBS - T).

Animal Resources Assistant, Antibodies Inc., CA (Jan 03 — June 03) Monoclonal and polyclonal antibody production in goats, rabbits, chickens and rodents, animal husbandry.