Sentences with phrase «mec1 kinase activity»

P significantly increased hypothalamic cAMP levels and cAMP - dependent protein kinase activity.

Keeping R and C separate can help sustaining kinase activity critical for relaxing smooth muscle.

The current thinking is that Pten's phosphatase activity counteracts PI3 kinase activity.

The kinase activity of RIPK1 mediates hypothermia and lethality in a mouse model of TNF - induced shock, reflecting the hyperinflammatory state of systemic inflammatory response syndrome (SIRS), where the proinflammatory «cytokine storm» has long been viewed as detrimental.

Disruption of Ca2 + - dependent cell - matrix adhesion enhances c - Src kinase activity, but causes dissociation of the c - Src / FAK complex and dephosphorylation of tyrosine - 577 of FAK in carcinoma cells.

Moreover; it has been observed that elevated calcium levels and interaction of the membrane bound DAG with protein kinase C (PKC), activates PKC's kinase activity, which is known to regulate the cellular activity of MAPK [41].

Axitinib, a drug used to treat renal cell carcinoma, has been shown to be effective at inhibiting the Abl kinase activity in patients with BCR - ABL1 (T315I).

It has been observed that mutated BRAF not only upregulates its own kinase activity, but also that of MEK and ERK, which in turn promotes cell proliferation [34].

So we looked to see whether middle T also had an associated protein kinase activity, and we were delighted to find that it did.»

To cite a few instances, polymerase chain reaction (PCR), a molecular method developed over three decades ago, has been widely applied in disease diagnosis, disease mechanism deciphering, and prognosis prediction; the elucidation of tyrosine kinase activity in cancer cells has led to the development of novel drugs for cancer treatment; and the identification of proteins and genetic molecules by molecular methods as biomarkers for disease diagnosis and prognosis has been drawing great interest.

Ablation of MEKK4 kinase activity causes neurulation and skeletal patterning defects in the mouse embryo.

KIBRA regulates Aurora kinase activity and is required for precise chromosome alignment during mitosis.

The Ser / Thr kinase activity of the Yersinia protein kinase A (YpkA) is necessary for full virulence in the mouse, mollifying phagocytes, and disrupting the eukaryotic cytoskeleton.

Quizartinib inhibits FLT3 kinase activity and induced about a 50 % composite response rate among patients with FLT3 - ITD mutations in phase I and II studies, said lead author Mahesh Swaminathan, MD of the University of Texas MD Anderson Cancer Center in Houston.

Fructose selectively modulates c - jun N - Terminal kinase activity and insulin signaling in rat primary hepatocytes..

ATM and ATR protein kinase activity confers cellular resistance to the cytotoxic effects of the anthelminthic drug niclosamide.

KIBRA regulates Aurora kinase activity and is required for precise chromosome alignment during mitosis % U http://www.jbc.org/content/early/2012/08/17/jbc.M112.385518.short.

Invasion of eukaryotic cells by Borrelia burgdorferi requires β1 integrins and Src kinase activity.

«Two groups had reported a kinase activity associated with the viral Src protein,» recalls Hunter: Marc Collett and Ray Erikson, then at the University of Colorado Medical Center, and Mike Bishop and Harold Varmus, then at the University of California, San Francisco.

Rapamycin forms an inhibitory complex with the FRB domain of mTOR and inhibits the kinase activities of mTOR protein (18, 19).

The mutation is acquired, is present in a variable proportion of granulocytes, alters a highly conserved valine present in the negative regulatory JH2 domain, and is predicted to dysregulate kinase activity.

Through interaction with the cdk / cyclin complexes, p21waf1 / cip1 modulates their kinase activity, resulting in cell growth arrest (10).

The basal and B - RAF - stimulated kinase activities of a third variant are unaltered but its activation by RAS is significantly reduced, suggesting that it may act in a dominant - negative manner to modulate pathway signaling.

Kapoor P, Bao Y, Xiao J, Luo J, Shen J, Persinger J, Peng G, Ranish J, Bartholomew B, Shen X. (2015) Regulation of Mec1 kinase activity by the SWI / SNF chromatin remodeling complex.

Kapoor P, Bao Y, Xiao J, Espejo A, Yang L, Bedford MT, Peng G, Shen X. (2015) Phosphorylation - Dependent Enhancement of Rad53 Kinase Activity through the INO80 Chromatin Remodeling Complex.

Disruption of Autophagy at the Maturation Step by the Carcinogen Lindane Is Associated with the Sustained Mitogen - Activated Protein Kinase / Extracellular Signal — Regulated Kinase Activity

«As a result, researchers have used the presence of IBs and heightened kinase activity as a proxy for measuring LRRK2's harmful effects, rather than measuring LRRK2 levels directly,» explained Dr. Finkbeiner, who is the associate director of neurological research at Gladstone as well as a professor at the University of California, San Francisco, with which Gladstone is affiliated.

However, PI 3 - kinase activity, a well - known driver of prostate cancer, was notably absent from the metastasized tumors.

Pim - 3, a Proto - Oncogene with Serine / Threonine Kinase Activity, Is Aberrantly Expressed in Human Pancreatic Cancer and Phosphorylates Bad to Block Bad - Mediated Apoptosis in Human Pancreatic Cancer Cell Lines

The mechanism by which rapamycin inhibits mTOR - raptor complex kinase activity is not completely understood.

ERK1 / 2 activity was inhibited using U0126, PI3K - AKT kinase activity was inhibited using LY294002 and JNK activity was inhibited using SP600125 (all were from Calbiochem, USA).

Similarly, inhibiting ABL kinase activity with the allosteric inhibitor GNF5 decreased TAZ protein abundance (fig.

These results demonstrate that ABL kinase activity is required for osteolytic metastasis in breast cancer and suggest that pharmacological inhibition of the ABL kinases may be an effective treatment for bone metastasis.

Kinase domain mutants of Bcr - Abl exhibit altered transformation potency, kinase activity, and substrate utilization, irrespective of sensitivity to imatinib

Studies by ours and other groups have shown that a number of EphA2 and EphA3 mutations inactivate Eph receptor canonical signaling by disrupting ephrin binding or kinase activity, consistent with a role of canonical signaling in tumor suppression.

He received his MSc and his PhD in Molecular Pharmacology and Oncology at the Institute for Higher Studies of the University of Ferrara exploring the role of LRRK2 kinase activity in mouse model of model of familial Parkinson's disease and performing studies focused on the phenomena underlying neurodegeneration.

It has been shown in imatinib - resistant CML that drug resistance conferred by mutations does not necessarily correlate with proliferative advantage and increased kinase activity.34 Other, non-activating mutations or drug - resistance mechanisms, might be acquired by tumor cells.

Binding to ephrin ligands on the surface of neighboring cells induces canonical signaling involving receptor clustering, autophosphorylation on tyrosine residues, and kinase activity - dependent downstream signaling.

It is likely that our RIPK2 inhibitor 1 may interfere with the kinase activity of these off targets indirectly by resolving the inflammation.

In 2005, the identification of an activating mutation in JAK2 (the V617F mutation) as a STAT5 - activating and disease - causing genetic alteration in a significant proportion of patients with myeloproliferative neoplasms (MPNs) has emphasized the oncogenic role of the JAK tyrosine kinases in hematologic malignancies.2 — 5 JAK2 is a member of the Janus tyrosine kinase family comprising three other mammalian non-receptor tyrosine kinases (JAK1, JAK3 and TYK2) that associate with cytokine receptors lacking intrinsic kinase activity to mediate cytokine - induced signal transduction and activation of STAT transcription factors.6 All JAKs share a similar protein structure and contain a tyrosine kinase domain at the C - terminus flanked by a catalytically inactive pseudokinase domain with kinase - regulatory activity, by an atypical SH2 domain and by a FERM domain that mediates association to the membrane - proximal region of the cytokine receptors.7, 8 Soon after the discovery of JAK2 V617F, we and others described that activating JAK1 mutations are relatively common in adult patients with T - cell acute lymphoblastic leukemia (ALL) and participate in ALL development allowing for constitutive activation of STAT5.9 — 11 Several STAT5 - activating JAK1 mutations were also reported in AML and breast cancer patients.10

Structure of the BRAF: MEK Complex Reveals a Kinase Activity Independent Role for BRAF in MAPK Signaling

We found that canonical signaling by the EphB4 receptor is low in breast cancer cells and that ephrin - induced stimulation of EphB4 kinase activity inhibits breast cancer cell malignancy in culture and tumor growth in vivo (Figure 1A) through inhibition of the CRK proto - oncogene.

A Harpin Binding Site in Tobacco Plasma Membranes Mediates Activation of the Pathogenesis - Related Gene HIN1 Independent of Extracellular Calcium but Dependent on Mitogen - Activated Protein Kinase Activity

In addition, at least some Eph receptors can also signal through non-canonical mechanisms that are independent of ligand binding and kinase activity, for example through interplay with other receptor tyrosine kinase families and with serine / threonine kinases.

These activities are independent of ephrin binding and / or kinase activity and their mechanism is not well understood but in some cases depends on Eph receptor phosphorylation on serine / threonine residues (red circle).

Analysis of RIPK2 kinase activity.

Furthermore, we determined that RIPK2 inhibitor 1 effectively inhibited KHM2 cells at an approximate IC50 of 5 — 10 nM, and kinase activity was somewhat sensitive to detergent conditions (Fig. 4, B and C).

For example, our past work showed that two conserved tyrosine phosphorylation sites in the juxtamembrane segment of the Eph receptors not only mediate association with binding partners but also regulate receptor kinase activity.

The prevailing hypothesis has been that misfolded LRRK2 boosts the activity of a type of enzyme called kinase, and that this heightened kinase activity is what drives cell death.

Activation of Stat3 in v - Src - transformed Fibroblasts Requires Cooperation of Jak1 Kinase Activity