Sentences with phrase «microinjection of»

This technology requires technical skills that are typically not offered by veterinary practices and includes aspiration of immature or mature eggs from mares using ultrasound - guided transvaginal aspiration (TVA) of follicles, in vitro culture of the eggs, micromanipulation and microinjection of eggs with a single selected sperm, and embryo culture in the laboratory, with freezing, and transfer of embryos to synchronized recipient mares.

High efficiency transformation by direct microinjection of DNA into cultured mammalian cells.

We identified an efficient OCT4 - targeting guide RNA using an inducible human embryonic stem cell - based system and microinjection of mouse zygotes.

Microinjection of membrane - impermeable molecules into single neural stem cells in brain tissue.

17 Capecchi MR. High Efficiency Transformation By Direct Microinjection of DNA into Cultured Mammalian Cells.

Using microinjection of adeno - associated viral vector bearing a transgene encoding FKBP1b into the hippocampus of aged male rats, we assessed the critical prediction that overexpressing FKBP1b should reverse Ca2 + - mediated manifestations of brain aging.

These 2D cultures also abolish the need for intraluminal microinjection of single organoids and facilitate studies of host - microbe or virus interactions or the action of microbial enterotoxins because the luminal surface is fully exposed to the mucosal medium (22,23).

«Conventional techniques of producing transgenic animals, such as microinjection of genes into eggs and the retroviral transduction of genes into embryos, often produce many animals that are mosaic, which means they do not contain the foreign gene in all their cells.

We offer quality custom generation of a wide range of mouse models developed by microinjection of Sv129 / Pas or C57BL / 6N ES cells into blastocysts or by DNA pronuclear injection.

Microinjection of wild - type human ACVR1 RNA into alk8 — / — zebrafish embryos rescued approximately 80 % of the injected embryos completely or partially (Figure 3, C and F), showing that human ACVR1 can function as a BMP type I receptor in this zebrafish model and substitute for Alk8.

The resulting gradients must drive a sizable centripetal flux of cAMP because direct microinjection of cAMP showed that it diffused readily.

Not exact matches

The processes of microinjection and micromanipulation of egg and sperm are making a wider array of new treatments possible.

Transgenic lines were prepared using microinjections into gonads of young adult N2 hermaphrodites as described (Tabara et al., 1999; Timmons, Court & Fire, 2001; Vohanka et al., 2010).

The level of fluorescence you saw in the validation assay should give an accurate indication of how many pups will contain a mutation and many of the mice with the mutation will be homozygous for it in every cell; however, if the microinjection was done as the pronuclei were fusing, you may have several mosaic mice.

When overexpressed together in developing Xenopus embryos by RNA microinjection, these genes can induce eye - like structures as defined by the expression of markers for some retinal cell classes [7].

Generation of genetically modified mice by random insertion of a transgene can be performed by pronuclear DNA microinjection.

Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided.

A flat tip microinjection pipette with an internal diameter of 15 — 18 µm was used for iPS cell injection.

Evi Soutoglou, researcher in molecular biology at the IGBMC received the bronze medal and Philippe André, in charge of the microinjection service of the «Institut Clinique de la souris» (PHENOMIN - ICS), the crystal medal

The repository of available techniques includes microinjection, particle bombardment («gene gun» technology), laser irradiation, electroporation, sonoporation, and magnetofection (Table 1).

Microinjection manipulation resulted in the increased apoptosis of spermatocytes in testes from intracytoplasmic sperm injection (ICSI) derived mice

This product was similarly purified in a low - melt agarose gel and used at 1 ng / μl along with 50 ng / μl of the unc - 36 (+) cosmid derivative RIp16 (gift of L Lobel) for microinjection into unc - 36 -LRB--) animals.