Sentences with phrase «microtubule binding»

Bacterial plasmid carrying the K18 fragment of human Tau containing four microtubule binding repeats (a gift from M. Margittai (University of Denver, Denver, CO) was overexpressed in Bl21 (D3) Escherichia coli bacteria and purified, as previously described (Meyer et al., 2014).

Not exact matches

Normally tau binds to microtubules — molecular conveyor belts that move chromosomes and vesicles within cells.

However, when they then «fixed» a specific hinge region of the dynein molecule that is known to connect dynein to its microtubule, they discovered that the dynein to spontaneously moved in its characteristic direction when force was applied at the ATP binding site, matching the way it moves in nature.

The researchers found that AS - 2 binds strongly to the kinesin motor, preventing it from sticking to a cell's monorails — that is, traveling along microtubules.

«Our study shows how EB proteins can either facilitate microtubule assembly by binding to sub-units of the microtubule, essentially holding them together, or else cause a microtubule to disassemble by promoting GTP hydrolysis that destabilizes the microtubule lattice,» says Eva Nogales, a biophysicist with Berkeley Lab's Life Sciences Division who led this research.

Through a combination of high - resolution cryo - electron microscopy (cryo - EM) and a unique methodology for image analysis, a team of researchers with Berkeley Lab and the University of California (UC) Berkeley has produced an atomic view of microtubules that enabled them to identify the crucial role played by a family of end - binding (EB) proteins in regulating microtubule dynamic instability.

They added a tubulin - binding protein known as Tau, that connects the HyPer protein to the microtubule structures.

His team found that, from its vantage point at the minus - end of an existing microtubule, Kinesin - 14 attaches to a second protein that is bound to the plus end of a growing microtubule.

When for the protein does not bind properly to the microtubules that form the cell's structure, it has a tendency to clump together, she explained, forming insoluble fibers in the neuron.

This competition between strain and binding to the microtubule is needed to guarantee processivity of this motor.»

Since its development, lattice light - sheet microscopy has been used to image numerous important events, such as single transcription factor molecules binding to DNA, hotspots of transcription, microtubule instability, protein distributions in embryos, and much more.

Each kinesin contains two «head» subunits, and each subunit contains two binding sites — one to grip and walk along microtubules and the other to bind ATP.

We demonstrated the technique on 20 different biological processes spanning four orders of magnitude in space and time, including the binding kinetics of single Sox2 transcription factor molecules, 3D superresolution photoactivated localization microscopy of nuclear lamins, dynamic organelle rearrangements and 3D tracking of microtubule plus ends during mitosis, neutrophil motility in a collagen mesh, and subcellular protein localization and dynamics during embryogenesis in Caenorhabditis elegans and Drosophila melanogaster.

For example, the protein PRC1 binds with microtubules, creating bundles of microtubules and cross-links between them.

The «parts list» in these processes is similar: Microtubules, semi-rigid tubes of protein, can serve within the cell as scaffolding, roadways, and a building material for machinery; some proteins serve as fasteners, binding and releasing other materials; and motor proteins use chemical energy to push and pull materials along microtubules, or move the microtubulesMicrotubules, semi-rigid tubes of protein, can serve within the cell as scaffolding, roadways, and a building material for machinery; some proteins serve as fasteners, binding and releasing other materials; and motor proteins use chemical energy to push and pull materials along microtubules, or move the microtubulesmicrotubules, or move the microtubulesmicrotubules themselves.

In addition to binding microtubules, centromeres have other functions, including sister chromatid cohesion and preventing microtubules from both poles attaching to the same chromatid.

The alpha and beta «tails» that protrude from the microtubule surface are known sites for modification, which in turn, determine which motors and associated protein will bind to the microtubule.

Identification of proteins that bind and control microtubule nucleation and dynamics during mitosis (Vernos group, Current Biology 2012, 2013, Nature Comm 2014, 2015, Current Biology 2015, J Cell Sci 2016, Mol Biol Cell 2016).

Tubulin - mediated binding of human immunodeficiency virus - 1 Tat to the cytoskeleton causes proteasomal - dependent degradation of microtubule - associated protein 2 and neuronal damage.

GAS2 - like proteins mediate communication between microtubules and actin through interactions with end - binding proteins.

GCP6 binds to intermediate filaments: a novel function of keratins in the organization of microtubules in epithelial cells.

Aurora A regulates the activity of HURP by controlling the accessibility of its microtubule - binding domain.

The spindle is made of microtubules (MT), molecular motors, and MT - binding factors, some of which show astounding complexity.

Tau is a protein that binds to microtubules and promotes stabilization of the cell's internal structure.