Sentences with phrase «mononuclear cell pellets»

Mononuclear cell pellets were separated from the diluted blood specimen by centrifugation with ficoll (400 g, 30 min) and the mononuclear cell layer was removed from the tube using a transfer pipette, resuspended in a phosphate buffered saline solution, and briefly centrifuged again.

Ficoll purified peripheral blood mononuclear cell pellets of the sort used in the current investigation are comprised of several different cell types (e.g., CD4, CD8, CD14, CD19, CD56)(Reinius et al., 2012).