Sentences with phrase «mononuclear cells from the blood»

Mononuclear cells from the blood were isolated during the OGTTs to analyze if these cells were producing molecules known as ROS (reactive oxygen species).

Not exact matches

Researchers analyzed peripheral blood mononuclear cells from a total of 18 children and adolescents in three matched groups: bipolar patients, unaffected offspring of bipolar parents and children of parents with no history of psychiatric disorders.

The protocol can be used to measure adaptive responses following the stimulation of whole blood samples or purified peripheral blood mononuclear cells (PBMCs) and includes a freezing step that allows the stimulated samples to be shipped from the collection site to the analysis facility.

There is evidence that this might cause chronic inflammation in the gut, Petri said, coming from work by Evan Newell of Stanford University and colleagues who looked at peripheral blood mononuclear cells in three year old children who had received the oral polio vaccine in the first months of their life.

We have also generated T - regs (CD4 + / 25high / 127low / --RRB- in vitro from donor AD - MSC and recipient peripheral blood mononuclear cells and these T - regs are infused in thymus of renal allograft recipients after kidney transplantation.

To find out, the researchers examined methylation patterns in iPSCs derived from the peripheral blood mononuclear cells of 16 donors, ages 21 to 100.

METHODS: Peripheral blood mononuclear cells obtained from patients who received AN1792 were stimulated in vitro either with beta - amyloid (Abeta) or various overlapping peptides of Abeta (1 - 42), followed by quantification of cytokine - secreting cells by enzyme - linked immunosorbent spot assay.

Peripheral blood mononuclear cells from both responders and non-responders to the drug were isolated and exposed to cell activators to induce an asthma - like state.

The Clinical Core of the Cleveland Alcohol Center can provide CAC investigators to access to de-identified biological samples (tissue biopsy, plasma / serum, urine, DNA and peripheral blood mononuclear cells (PBMCs) from patients with different stages of alcoholic liver disease, as well as healthy control subjects.

T - cell responses specific to the carboxy terminus of Abeta elicited from patients» peripheral blood mononuclear cells in an earlier multiple dose study (Study 102) were Th2 biased, while those from Study 201 were biased toward a proinflammatory Th1 response.

For this study, Encode will isolate peripheral blood mononuclear cells from patients and generate gene expression profiles of cells through in vitro exposure to the candidate drug.

In October 2009, Lombardi et al. reported finding a gammaretrovirus called xenotropic murine leukemia virus - related virus (XMRV) in peripheral blood mononuclear cell (PBMC) DNA from about 67 % (68/101) of CFS patients compared to only 3.6 % (5/218) of healthy persons using PCR testing [11].

A recent study by Lombardi et al. [1] describing a gamma - retrovirus infection in 68 of 101 chronic fatigue syndrome (CFS) patients was notable not only for its claim of a new viral aetiology of a hitherto controversial disease, but also for the fact that proviral DNA could be amplified from the peripheral blood mononuclear cells (PBMC) of 3.75 % (8/218) of the healthy controls.

Methods: We collected samples of peripheral blood mononuclear cells and intestinal tissues from healthy individuals (controls, n = 13 - 30) and patients with inflammatory bowel diseases (n = 119; 59 with ulcerative colitis and 60 with Crohn's disease).

GABA Regulates Release of Inflammatory Cytokines From Peripheral Blood Mononuclear Cells and CD4 + T Cells and Is Immunosuppressive in Type 1 Diabetes.

Generation of integration - free human induced pluripotent stem cells from postnatal blood mononuclear cells by plasmid vector expression.

Conclusions: In an analysis of peripheral blood mononuclear cells and intestinal tissues from patients with inflammatory bowel diseases vs controls, we found that reactivity to intestinal bacteria is a normal property of the human CD4 + T - cell repertoire, and does not necessarily indicate disrupted interactions between immune cells and the commensal microbiota.

Resection samples from 20 IBD and control patients were used for measles hybrid capture followed by RT - PCR, in addition to peripheral blood mononuclear cells (PBMCs) from 13 IBD and control patients.

Mononuclear cell pellets were separated from the diluted blood specimen by centrifugation with ficoll (400 g, 30 min) and the mononuclear cell layer was removed from the tube using a transfer pipette, resuspended in a phosphate buffered saline solution, and briefly centrifMononuclear cell pellets were separated from the diluted blood specimen by centrifugation with ficoll (400 g, 30 min) and the mononuclear cell layer was removed from the tube using a transfer pipette, resuspended in a phosphate buffered saline solution, and briefly centrifmononuclear cell layer was removed from the tube using a transfer pipette, resuspended in a phosphate buffered saline solution, and briefly centrifuged again.