Sentences with phrase «mutant phenotypes»

Abnormal Physiological and Molecular Mutant Phenotypes Link Chloroplast Polynucleotide Phosphorylase to the Phosphorus Deprivation Response in Arabidopsis

Here we offer additional explanations for the low frequency of mutant phenotypes.

Collectively, these findings augment the endoderm GRN and reveal a panel of target genes underlying the Ta, Tbx16, and Mixl1 mutant phenotypes.

We identified mutant phenotypes for 1,722 genes, about two - thirds of which were not previously associated with a phenotype.

Use of Illumina sequencing to identify transposon insertions underlying mutant phenotypes in high - copy Mutator lines of maize

Moreover, we have often identified individual mRNA targets whose in vivo deregulation is critical for driving mutant phenotypes.

Next, we explored whether overexpressing GBP1 and GBP2 specifically in the fat body rescues the double null mutant phenotypes.

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., «cell shape»), a qualifier (e.g., «abnormal»), a mutant type (e.g., null), strain background, and a reference.

The application of transgenesis and other genetic methods - in conjunction with total genome sequence and database information on gene expression patterns, morphological changes during development, and mutant phenotypes - should significantly enhance our ability to unravel the multilayered networks that control gene expression and differentiation.

In yeast and Caenorhabditis elegans, the majority of ORFs revealed by genomic sequencing have not been associated with mutant phenotypes, even in genomic regions that have been subjected to saturation mutagenesis (36 - 37).

To understand the function of such silent genes, it will be necessary to obtain null mutations, just as in the case of genes causing obvious mutant phenotypes.

The finding that bsl1 - 1 and bsl1 - 2 fail to complement and have SNP variation affecting dramatic changes in the coding capacities of the same gene demonstrate that loss of Sevir.7 G140700 function is responsible for the bsl1 mutant phenotypes.

Expressing GLKs increased the chlorophyll content of fruit, whereas SlGLK2 suppression recapitulated the u mutant phenotype.

Wild - type ACVR1 mRNA (6 — 60 pg) at the 1 - cell stage fully (as in A) or partially rescued the mutant phenotype (C1 dorsalized; C).

Such cell lines are now opening the opportunity to carry out «large scale» functional genomics screens in order to identify the genes able to revert the mutant phenotype of the cells.

The technical evaluation of projects may require the provision of additional data such as information on the genetic modification of your mutant mouse line if applicable (e.g. affected gene, MGI ID of the gene, type of mutation, ES - cell line used, genetic background (e.g. number of backcross generations), safety level, description of DNA modification, vector, remaining non-recipient DNA, donor organism), mutant phenotype (s), special housing or care requirements, current sanitary status, and intellectual property rights (who generated the mouse line, owner of the mouse line)

(B) In contrast, embryos from torY9 psqrum double mutant females show terminal defects resembling the psqrum single mutant phenotype.

In contrast, all embryos from homozygous torY9 rum double mutant females show terminal defects resembling the rum single mutant phenotype (Figure 2B; n = 50).

Characterization of complementing and non-complementing YAC transgenic families revealed that correction of the inv mutant phenotype was concordant with integration and intact expression of this novel gene, which we have named inversin (Invs).

Although transgene insertion was accompanied by neighbouring deletion and duplication events, our YAC phenotype rescue studies indicate that the mutant phenotype results from the deletion.

It is a mutant phenotype.

The phenotype of unc - 9 unc - 7 double mutants is no more severe than either single mutant phenotype, consistent with the co-localization of UNC - 9:: GFP and UNC - 7S.

We applied our technology to several epigenetic regulators, recovering bi-allelic targeted clones with a high efficiency of 60 % and used Flp recombinase to restore expression in two null cell lines to demonstrate how our system confirms causality through mutant phenotype reversion.

This is demonstrated in Figures 1 and 2, whereby restoring endodermal cell numbers does not abrogate the toddler mutant phenotype.

Not exact matches

This study found that when cacophony and its related gene straitjacket are mutated, they cause similar degenerative phenotypes in the mutant neurons as autophagy mutants.

We saw this very curious phenotype — this mutant worm with this heightened sense of smell — and remembered it from previous experiments dealing with something totally different.»

Furthermore, we now know that the energy stores in mutant flies are normal and that the starvation - induced sleep suppression phenotype is not due to increased nutrient storage.»

«As it has only one set of chromosomes, it is very easy to find interesting mutants as all you have to do is to alter a single allele to produce a phenotype,» explains Oscar Fernández - Capetillo, head of the Genomic Instability Group and the leader of the research project.

Researchers found 91 conditions that had uniquely positive or negative effects on one of the mutants, and a cluster analysis of 37 commonly beneficial compounds and 57 commonly detrimental compounds revealed three separate phenotype groups.

Even classical Mendelian disorders have different clinical phenotypes that are a consequence of polymorphic or mutant disease ‐ modifying genes and their interactions with environmental factors.

Embryos exhibited a strongly dorsalized C5 phenotype in the absence of BMP ligands (uninjected, n = 34; bmp2b MO, n = 34), whereas mutant ACVR1 caused ventralization of embryos deficient in bmp7 (n = 126) or both bmp7 and bmp2b (n = 103).

Thus, the range of expression levels from these two mutant alleles affected their ability to complement some phenotypes (hair).

A total of 22 mouse mutant lines can be characterised through a broad based primary phenotyping pipeline in all the major adult organ systems and most areas of major human disease.

Finally we aim at identifying phenotype specific transcriptional targets of mutant HTT (mut - HTT) or HTT loss of function in relevant neuronal / glial populations.

Also, given my earlier analysis of arousal phenotypes of DopR mutants, we have tested their performance in the visual arena, and these mutant animals are indeed compromised in their ability to perform visual tasks.

The phenotyping pipeline that will be used within INFRAFRONTIER - I3 builds on the EUMODIC pipeline and uses a cohort of seven female and seven male mutant mice for a comprehensive first - line phenotyping analysis.

The IMPC envisages a ten year programme to undertake a broad - based, systematic genome - wide phenotyping project of knockout mice generated from the embryonic stem cell mutant resources developed by the International Knock - out Mouse Consortium (IKMC).

Phenotype data from 449 mutant alleles were acquired, representing 320 unique genes, of which half had no previous functional annotation and data from over 27,000 mice were captured, finding that 83 % of the mutant lines are phenodeviant, with 65 % demonstrating pleiotropy.

◊ to set standards for systemic phenotyping of mouse models and for archiving and distribution of mouse mutants in Europe.

Current efforts are focused on defining the molecular mechanism underlying the embryonic lethal phenotype of ADAR1 null mutant mice and identification of the dsRNA (s), which triggers the MDA5 - MAVS - IFN pathway.

Each mutant line will undergo a broad suite of high - throughput tests to identify developmental, anatomical, physiological, behavioural and pathological phenotypes.

Treated seedlings showed severe reduction in height, erect leaf angles, and delayed flowering, consistent with phenotypes typical of BR biosynthesis mutants (Clouse and Sasse, 1998)(Figure 5A).

(B) Compared with wild - type A10.1 (left), bsl1 - 1 (right) and bsl1 - 2 (middle) mutant panicles are skinnier and have reduced bristle phenotypes.

While similar phenotypes observed in bsl1 have been described for d11 mutants in rice (e.g., semidwarf stature, small seeds, and clustered panicle branches; Tanabe et al., 2005; Wu et al., 2016), the lack of bristles is a phenotype unique to S. viridis.

A double mutant for the CYP85A1 and CYP85A2 genes of Arabidopsis exhibits a brassinosteroid dwarf phenotype

As with most other observed phenotypes, spikelet - to - bristle ratio was intermediate in the bsl1 - 2 mutant compared with the wild type and bsl1 - 1 (Supplemental Figure 4).

Furthermore, AICAR, an analogue of AMP, rescued glial differentiation of Lkb1 - deficient NCC and corrected the Schwann cells and ENS phenotypes of Lkb1 mutant mice.

Thus, chemical disruption of BR biosynthesis recapitulated the bsl1 mutant inflorescence phenotypes.

We can not rule out the influence of other SNPs fixed in the mutant populations on seemingly unrelated phenotypes, such as the elongated panicle phenotype displayed by bsl1 - 2 (Figure 1B), since several rounds of backcrossing were not performed.

One mutant produced few to no bristles while the other displayed a weaker phenotype, producing substantially fewer and shorter bristles than the A10.1 reference line (Supplemental Table 1 and Supplemental Figures 1B and 1C).