Sentences with phrase «n. vitripennis»

Christine Elsik kindly provided us with the GLEAN6 consensus gene set for N. vitripennis prior to its publication.

However, only the correlation between the recombination rate and ω when comparing N. vitripennis with N. longicornis proved to be statistically significant.

The mapped markers enabled us to arrange 265 scaffolds of the Nasonia genome assembly 1.0 on the linkage map, representing 63.6 % of the assembled N. vitripennis genome.

Our data show an overall negative trend between the recombination rate and both the amino acid distance (dA) and the ratio of non-synonymous to synonymous substitutions per site (ω) when comparing N. vitripennis exon sequences with those of N. giraulti and N. longicornis.

We are grateful to J. Romero - Severson for developing the N. vitripennis BAC library; Stephen Richards and the Baylor Human Genome Sequencing center for sequencing of BAC clones as part of the Nasonia genome project; Rhitoban Raychoudhury, Jen Traggis, Laramy Enders, and Adityarup Chakravorty for assistance with laboratory work; Heinrich Jasper for advice on qPCR; and Claude Desplan for comments on the manuscript.

To positional clone this major sex - specific wing QTL and to more precisely describe its phenotypic effects we (a) reduced the size of the introgressed sequence flanking the ws1 locus to a 40kb segment (see fine - scale mapping and cloning below) and (b) backcrossed the introgressed ws1g segment into a standard N. vitripennis strain (AsymCX) genetic background for > 10 generations.

Wing measurements were conducted using the inbred N. vitripennis strain AsymC and inbred N. giraulti strain R16A; these data are also reported in [15].

Here we investigate the genetic basis of male - specific differences between two species of Nasonia, N. vitripennis and N. giraulti.

Gene expression experiments were conducted with the N. vitripennis AsymCX strain used for genome sequencing [10], which was derived from AsymC by multiple generations of sib - mating.

Male wing size increases by 45 % through cell size and cell number changes when the ws1 allele from N. giraulti is backcrossed into a N. vitripennis genetic background.

Wild - type N. vitripennis and N. giraulti are also referred to as ws1vV and ws1gG in the text.

Regions with significant bacterial identity (E value < 1e − 5) were then compared to a second database containing representative animal genomes (Homo sapiens, Mus musculus, Rattus rattus, Monodelphis domestica, Gallus gallus, Xenopus laevis, Drosophila melanogaster, Anopheles gambiae, N. vitripennis, A. mellifera, Daphnia magna) to obtain a corresponding «animal» BLASTN E value score.

Eradicating the full complement of the wasps» microbes allowed hybrids of N. vitripennis and its two distant relatives to survive.

The N. vitripennis immune response is of particular interest given the complex biology of bacterial associations documented in this species.

In this study, we have characterized the immune - inducible transcriptome in N. vitripennis using RNA - sequencing, and identified genes that respond transcriptionally to infection.

As previously reported, N. vitripennis possesses all the main components of the Imd, Toll, and JAK / STAT pathways [15], and this new study does not significantly update the known signaling repertoire (Table S1).

Nonetheless, 3 of the 12 PGRPs in N. vitripennis are induced by infection, along with 2 lectins (Table S1).

Together, these form the most complete annotation of immune - related genes in N. vitripennis to date, and represent one of the first genomic - scale annotations of novel immune - induced transcripts in Hymenoptera.

We aligned our RNA - seq reads to the N. vitripennis reference genome version 1.0 [15], available from NasoniaBase.

If N. vitripennis females ultimately come to prefer the RS - RR pheromone blend, they must eventually start associating it with males of their own species and evolve a way to recognize it.

When the scientists analyzed the N. vitripennis male sex pheromone, they found it contained two important chemicals, which they call RS and RR.

N. vitripennis females preferred a blend of RS and RR, but a whiff of RS alone was enough to get them in the mood, the researchers report online today in Nature.

Although the two closely related species can interbreed with some success, if either mates with N. vitripennis, more than 90 per cent of the offspring die.

The third species, N. vitripennis, is less similar.

Bordenstein believes that this mismatch can drive a wedge between two groups of organisms until their offspring can no longer survive, ultimately resulting in a separate, new species akin to N. vitripennis.

To test their idea, they raised the hybrid offspring of N. vitripennis and each of the other wasp species in a germ - free environment, eliminating any signs of gut bacteria that might be toxic to the wasps.

Not exact matches

Males of the emerging genetic model wasp Nasonia vitripennis have small wings and do not fly, while males of the closely related species N. giraulti have large wings and do fly.