Sentences with phrase «nscs as a negative control»

The left hind footpad served as a negative control and was injected with 25 μl of HBSS without cells.

PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Can be used as a negative control for other p - BABE - puro derived HOS CD4 cell lines.

An isotype control for the secondary antibody was used as a negative control in both samples.

Calpain inhibitor (1 μl) added to treated cell lysate was used as a negative control.

A sample without a primary antibody can be used as a negative control to distinguish specific and non-specific signal from the secondary antibody.

Furthermore, samples that have been genetically manipulated to alter the target's levels (e.g., CRISPR) can be used as a negative control.

As a negative control, unedited parental cells were grown in parallel for the same amount of time.

(A) GUS activity with and without 10 µm of the TNT ligand in paired leaves from transgenic plants serving as negative controls and transgenic plants containing the complete signal transduction system.

[14, 15] As a negative control, a second pair of TMA slides was incubated overnight in ACE block only.

By contrast, while 5D12 was not designed as a negative control, it proved significantly less effective than the other candidates, for reasons that are not entirely clear.

While we could not use the XT slides with secondary antibody only as a negative control (due to the limited material), we did stain smears of ticks fed on clean mice using all antibodies (primary and secondary).

Mouse ES (mES) cells were used as a negative control.

si - Control targeting EGFP sequence was used as a negative control.

Red histograms on each graph show target cells incubated with supernatant from unmodified HB1.F3 NSCs as a negative control.

As a negative control, medium alone (100 µL) was added.

A pool of normal human plasma was used as a negative control (NC).

Vero E6 cells without virus inoculation and HeLa cells without ACE2 were used as negative control.

The β - catenin / TCF binding site for c - MYC was amplified as a positive control, and the genomic sequence for β - actin was amplified as a negative control.

In all our immunohistochemical analyses of PRLR in mouse mammary tissue, we used PRLR - deficient mammary glands as a negative control to test the specificity of the staining.

Not exact matches

«Divergence in external tariffs and internal rules as well as absence of common institutions and a shared legal system, necessitates checks and controls to uphold the integrity of the EU single market as well as of the U.K. market... This, unfortunately, will have negative economic consequences,» it said.

«The consortium of 40 + banks (known as R3cev) which aims to do just that will inevitably develop something which: is permissioned (for users and developers like the apple app store), privatized, has fees, will not be entirely transparent to everyone, will not be open - source, it will definitely be inflationary to accommodate monetary policy of debasement and fractional reserve schemes, it will facilitate negative interest rates, central control of accounts for suspension / freezing of funds, bail - ins, bail outs, capital controls and transactions will include the identity of both sender and receiver and store that information in a centralized location for the convenience of hackers.»

Sabet, who worked in the White House's Office of National Drug Control Policy during President Obama's first term and serves as director of the Drug Policy Institute at the University of Florida, said the negative consequences of marijuana legalization include advertising aimed at kids, an increase in drugged - driving incidents and a spillover of marijuana from Colorado into surrounding states, where the drug remains illegal.

Concerns over negative economic impacts, such as a widening trade deficit with China, increased control of certain sectors by Chinese state - owned enterprises (SOEs), and intensified competition in the labour market for mid-skilled and less educated Canadian workers in particular; [1]

For example, ISS views as negative a single trigger, such as automatic vesting upon a change in control.

In a richly valued market, that sort of risk control is most appropriately established using call options having a strike price situated at about the point where various trend - following measures would turn negative — what is known in finance as a «contingent position» because the position creates its own exit if the market deteriorates further without an interim recovery - and particularly if it deteriorates abruptly.

Tony Jones, Brian McLaren and the Emergent Crowd have sent many a «hired hand» to post negative posts here about Tony Jones» ex-wife Julie, David, and to try to get control again of the narrative (as everybody has noticed).

They are «dimly conscious» in two senses: (1) as experiences, they do not normally rise to the stature of conscious centers competing for control of the organism, but they have appetitions and aversions in their own right so that it seems appropriate to label them «dimly conscious»; (2) they are perceived only dimly by the members of the regnant society, i.e., the regnant society has these particular occasions as dim, vaguely felt, negative «scars» on the data of what is clearly perceived in full consciousness.

As a result of this all too typical pattern of unjust land distribution, for example, 77 percent of the land in El Salvador, where fourteen families control the wealth, is suffering from erosion, according to a recent study.25 A more just distribution of land would greatly reduce the negative impact on the environment.

There were her black - and - white negative thoughts, her rejection of help, her insistence on a definition of life as being able to take care of oneself, her use of objectified terms (such as the new life stage of «miserable existence» to replace merely «feeling miserable»), her unsolicited speaking for others, her legalistic analysis of the problem of euthanasia and doctor - assisted suicide, her exaggeration of minor and temporary discomforts, her refusal to accept family support — cumulatively resulting in her choice to be «in control» and die.

It doesn't preclude the state from giving material support to the Church, so long as «support» doesn't turn into control or the negative treatment of religious minorities.

This feminist psychiatrist predicts «extremely negative psychological sequelae» should the «fundamental right to abortion as a legitimate option in attaining control over one's own bodily self be suddenly renounced.»

Genetically engineered plants designed to control pests can have negative side - effects on beneficial insects and further non-target organisms as well.

View the negative situation as a single event that is not connected to other potential events and that is caused largely by external factors over which you can have little control.

Women run 5 to 7 times the risk of death with cesarean section compared with vaginal birth.14, 29 Complications during and after the surgery include surgical injury to the bladder, uterus and blood vessels (2 per 100), 30 hemorrhage (1 to 6 women per 100 require a blood transfusion), 30 anesthesia accidents, blood clots in the legs (6 to 20 per 1000), 30 pulmonary embolism (1 to 2 per 1000), 30 paralyzed bowel (10 to 20 per 100 mild cases, 1 in 100 severe), 30 and infection (up to 50 times morecommon).1 One in ten women report difficulties with normal activities two months after the birth, 23 and one in four report pain at the incision site as a major problem.9 One in fourteen still report incisional pain six months or more after delivery.9 Twice as many women require rehospitalization as women having normal vaginal birth.18 Especially with unplanned cesarean section, women are more likely to experience negative emotions, including lower self - esteem, a sense of failure, loss of control, and disappointment.

Tough summarizes key research, such as the Adverse Childhood Experience Study, a project of the Centers for Disease Control and Kaiser Permanente, which revealed a stunning correlation between traumatic childhood events and negative adult outcomes.

The overwhelming focus on negative aspects of life and conflict in the news colors our perception as well and can either numb or desensitize us or increase our general anxiety load, or leave us feeling helpless over events outside our control.

For example: an 18 month old throwing a spoon on the floor could be seen as trying to disrupt or take control, which would assign the child more negative motive, than if the parent were to also consider tiredness and natural developmental challenges of this age, which would be seen as normal and inevitable testing of limits.

As a built in feature of the Hazelbaker FingerFeeder, this infant control can be augmented by a gentle squeeze of the milk container should the baby not be able to generate adequate negative pressure to draw milk from the device.

As he gets older and gains more control and understanding, this sort of negative behavior will subside.

As with neurobehavior, effects on breastfeeding are poorly studied, and more recent randomized controlled trials comparing exposure to epidural and opiate drugs are especially misleading because opiates have a well - recognized negative effect on early breastfeeding behavior and success.103 — 107

Like, yes they have gestational diabetes, but they're controlling it well enough as evidenced by the meter to keep negative effects from happening?

In closing their article, Price et al. stated that organizations, such as the Australian Breastfeeding Association and the Australian Infant Mental Health Association, were unduly negative towards controlled - crying techniques and based their positions on research that had not been «updated since the mid-2000s.»

The NRA, infuriated over Cuomo's Safe Act, which is billed as the toughest gun - control legislation in the nation, is preparing to give the Cuomo / Hochul ticket a negative rating while backing Astorino.

With Kurantowicz as director, Voters for Good Government made a splash in 2012 by spending about $ 300,000 on a last - minute burst of negative ads and mailings aimed at trying to defeat a half - dozen Democrats and flip control of the state Senate.

Asked if Democrats» winning control of Congress in next year's elections would benefit rather than hurt the country, more see it as a positive than negative — but opinions are not resolute.

«Unlike many other things people can't quite define but «know when they see it,» identification of dogs as pit bulls can trigger an array of negative consequences, from the loss of housing, to being seized by animal control, to the taking of the dog's life,» she said.

«The most cost - effective measure we could take is the control of feral pigs at a cost of $ 2 million per annum in targeted location across the Basin, as they have a negative impact on both native plants and animals,» she said.

For 5 months, every comment submitted by a user randomly received an «up» vote (positive); a «down» vote (negative); or as a control, no vote at all.

Participants in a control group practiced a different technique known as cognitive reappraisal, in which they learned to generate fewer negative thoughts.