Sentences with phrase «nanopore sequencing»

I mainly work on data generated by whole genome sequencing, whole exome sequencing, targeted resequencing, RNA - seq and Nanopore Sequencing using MinION.

Real - time nanopore sequencing and analysis of plant genomes in a tent in Snowdonia National Park

Recent advances in nanopore sequencing technology have led to a substantial increase in throughput and sequence quality.

Use of Unamplified RNA / cDNA — Hybrid Nanopore Sequencing for Rapid Detection and Characterization of RNA Viruses

Nanopore sequencing gets a boost with accurate error modelling and variant - calling tools for Oxford Nanopore Technology's highly anticipated MinION platform.

Detection of subclonal L1 transductions in colorectal cancer by long - distance inverse - PCR and Nanopore sequencing

Same - day genomic and epigenomic diagnosis of brain tumors using real - time nanopore sequencing

In experimental and prototype nanopore sequencing devices it has been shown that the electrolytic current signals are sensitive to base modifications, such as 5 - methylcytosine.

Motivation: Nanopore sequencing may be the next disruptive technology in genomics, due to its ability to detect single DNA molecules without prior amplification, lack of reliance on expensive optical components, and the ability to sequence very long fragments.

Nanopore sequencing provides a rapid, cheap and portable real - time sequencing platform with the potential to revolutionise genomics.

A new hybrid approach for MHC genotyping: high - throughput NGS and long read MinION nanopore sequencing, with application to the non-model vertebrate Alpine chamois (Rupicapra rupicapra)

Using MinION nanopore sequencing to generate a de novo eukaryotic draft genome: preliminary physiological and genomic description of the extremophilic red alga Galdieria sulphuraria strain SAG 107.79

Simultaneous detection of DNA and RNA virus species involved in bovine respiratory disease by PCR - free rapid tagmentation - based library preparation and MinION nanopore sequencing

Oryza coarctata plants, collected from Sundarban delta of West Bengal, India, have been used in the present study to generate draft genome sequences, employing the hybrid genome assembly with Illumina reads and third generation Oxford Nanopore sequencing technology.

In nanopore sequencing devices, electrolytic current signals are sensitive to base modifications, such as 5 - methylcytosine (5 - mC).

Nanopore sequencing of full - length BRCA1 mRNA transcripts reveals co-occurrence of known exon skipping events

Hand - held rapid whole genome nanopore sequencing to predict neisseria gonorrhoeae antibiotic susceptibility: steps towards clinic based tailored antimicrobial therapy

Unbiased Strain - Typing of Arbovirus Directly from Mosquitoes Using Nanopore Sequencing: A Field - forward Biosurveillance Protocol

Rapid draft sequencing and real - time nanopore sequencing in a hospital outbreak of Salmonella

Nanopore sequencing enables near - complete de novo assembly of Saccharomyces cerevisiae reference strain CEN.PK113 - 7D

MinION Nanopore Sequencing Enables Correlation between Resistome Phenotype and Genotype of Coliform Bacteria in Municipal Sewage.

Oxford Nanopore Technologies» nanopore sequencing device, the MinION, holds the promise of sequencing ultra-long DNA fragments > 100kb.

Identification of a novel species of papillomavirus in giraffe lesions using nanopore sequencing

Nanopore sequencing, a novel genomics technology, has potential applications for routine biosurveillance, clinical diagnosis, and outbreak investigation of virus infections.

NanoOK: Multi-reference alignment analysis of nanopore sequencing data, quality and error profiles

Dr Andrew Kilianski presents a talk for the MinION Community on Real - time Genomic Characterization of Viral Threat Agents using Nanopore Sequencing.

Nanopore sequencing has been available to customers for a little over three years.

Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop - mediated isothermal amplification method and MinION ™ nanopore sequencing

Detection of active transposable elements in Arabidopsis thaliana using Oxford Nanopore Sequencing technology

A talk by Stephanie Hao of Johns Hopkins University Applications of Nanopore Sequencing for Infectious Disease Detection at London Calling 2016.

Noninvasive Prenatal Testing by Nanopore Sequencing of Maternal Plasma DNA: Feasibility Assessment

Oxford Nanopore sequencing, hybrid error correction, and de novo assembly of a eukaryotic genome

NanoOk — Flexible, multi-reference software for pre - and post-alignment analysis of nanopore sequencing data, quality and error profiles

Nanopore sequencing is a promising technique for genome sequencing due to its portability, ability to sequence long reads from single molecules, and to simultaneously assay DNA methylation.

npReader (jsa.np.f5reader) is a program that extracts Oxford Nanopore sequencing data from FAST5 files, performs an initial analysis of the date and streams them to real - time analysis pipelines.

Nanopore sequencing instruments measure the change in electric current caused by DNA transiting through the pore.

We report unbiased metagenomic detection of chikungunya virus (CHIKV), Ebola virus (EBOV), and hepatitis C virus (HCV) from four human blood samples by MinION nanopore sequencing coupled to a newly developed, web - based pipeline for real - time bioinformatics analysis on a computational server or la

«Analyzing - omic Instability in breast cancer with nanopore sequencing of patient - derived organoids»

Using nanopore sequencing, metagenomic detection of viral pathogens directly from clinical samples was performed within an unprecedented < 6 hours sample - to - answer turnaround time and in a timeframe amenable for actionable clinical and public health diagnostics.

We report unbiased metagenomic detection of chikungunya virus (CHIKV), Ebola virus (EBOV), and hepatitis C virus (HCV) from four human blood samples by MinION nanopore sequencing coupled to a newly developed, web - based pipeline for real - time bioinformatics analysis on a computational server or laptop (MetaPORE).

Introduction to ONT devices and latest technology Wet lab training and best practices for sample quality and library preparation for Nanopore sequencing Running MinKNOW and real - time sequencing data handling Introduction to basecalling and analysis tools (ONT and opens source) for analysis of ONT data

After this course you should be able to: - Prepare libraries from genomic DNA for amplicon or whole genome approaches to nanopore sequencing - Run ONT devices and assess sequencing performance during a run - Understand the basics of ONT data handling and analysis - Analyse and interpret ONT amplicon data and whole genomic data from bacterial samples

This course is directed towards molecular biologists, bioinformaticians, scientists and technicians who want to apply Oxford Nanopore Sequencing technology in their labs.

That Nanopore sequencing has the ability to revolutionize sequencing is recently demonstrated by KeyGene's publication: The megabase - sized fungal genome of Rhizoctonia solani assembled from nanopore reads only.

The company started therefore already in 2014 with investing in Nanopore sequencing in order to gain and build expertise in this area.

KeyGene's researchers involved in Nanopore sequencing are very enthusiastic about the results and possibilities of the technology:

The goal of this course is to teach the fundamentals of nanopore sequencing using the Oxford Nanopore Technologies (ONT) platform.

To address this challenge we developed the poreFUME workflow that deploys functional metagenomic selections and nanopore sequencing to resistome mapping.

Title: Analysis Methods for Nanopore Sequencing Data Abstract: Over the last four years nanopore - based sequencing instruments have become widely available.

This presentation will review methods for which Nanopore sequencing and real - time antimicrobial resistance gene analysis can help to address the threat of CRO as a diagnostic tool in the Medical Microbiology Laboratory.