Sentences with phrase «nematostella mutant lines»

The mutant line methuselah (mth) displayed approximately 35 percent increase in average life - span and enhanced resistance to various forms of stress, including starvation, high temperature, and dietary paraquat, a free - radical generator.

Dubcovsky and his colleagues chemically induced random genetic mutations in thousands of wheat seeds and began developing and characterizing their collection of wheat mutant lines more than five years ago.

Focusing on this small portion of each plant's genome, the team sequenced 400 billion bases of DNA using sophisticated sequencing technology to analyze the plants that grew from the mutated seeds — a total of 2,735 mutant lines.

And the genetic makeup of the flies also mattered, because lifelong antibiotic treatment caused one normally long - lived mutant line to die at the same age as normal flies given antibiotics — in other words, its life span was extended only in the presence of bacteria, the researchers report in this week's issue of Proceedings of the National Academy of Sciences.

Without a working version of the gene encoding SWEET4c, such as in the mutant lines provided by the UniformMu resource (Don McCarty and Karen Koch at the University of Florida), sugars were not delivered to the seeds and the backpack was empty (in science jargon: empty pericarp).

A total of 22 mouse mutant lines can be characterised through a broad based primary phenotyping pipeline in all the major adult organ systems and most areas of major human disease.

Phenotype data from 449 mutant alleles were acquired, representing 320 unique genes, of which half had no previous functional annotation and data from over 27,000 mice were captured, finding that 83 % of the mutant lines are phenodeviant, with 65 % demonstrating pleiotropy.

Each mutant line will undergo a broad suite of high - throughput tests to identify developmental, anatomical, physiological, behavioural and pathological phenotypes.

Mouse mutant lines can be tested through a broad based primary phenotyping pipeline in all the major adult organ systems and most areas of major human disease.

If a mouse mutant line was provided by selected applicants for a phenotyping project, sharing of the mouse line is also required and facilitated by the INFRAFRONTIER / EMMA repository.

The service provided consists of a comprehensive first line phenotyping of a mutant line with appropriate control.

In the IMPC phase 2 a significant fraction of mutant lines will be aged and re-phenotyped to identify genes involved with late - onset disease.

The core services of INFRAFRONTIER comprise the systemic phenotyping of mouse mutants in the participating mouse clinics, and the archiving and distribution of mouse mutant lines by the European Mouse Mutant Archive (EMMA)(www.infrafrontier.eu).

The funded access unit covers the derivation of a single mouse mutant line into a germ - free condition.

Not exact matches

Much of c - store discussion surrounding Monster over the past year concerned the uneven rollout and performance of the brand's new «super soda» line called Mutant; this year, the line will be getting a new clear, sugar - free, zero - calorie SKU called White Lightning to complement the bright green original and Red Dawn varieties.

By screening this population for mutants with an interesting appearance, two lines were found that gave beautiful spotty leaves.

Making Mutant Beach Hoppers: Beach hopper embryos (top), half a millimeter across, are lined up and prepared for an injection by Erin Jarvis, a graduate student at the University of California, Berkeley.

The researchers also examined BRAF mutant melanoma cell lines, and found that BRAF inhibition induced autophagy by way of an endoplasmic reticulum (ER) stress response.

After exploiting a technology that allowed them to activate each of nearly 16,000 genes individually in human melanoma cell lines containing mutant BRAF, the authors then treated the panel of cells with the drugs and monitored which cells showed altered drug sensitivity.

The mutants that Ambros and his colleagues worked with were all descendants of that original line.

We postulate that HD mutant hPSC (embryonic or induced) lines can constitute such models and would be key to the exploration of the molecular mechanisms of the disease and ultimately allow the screening of therapeutic compounds endowed with a therapeutic potential.

Experimental models include yeast, mammalian and human cell lines, mutant mouse models and tumour samples.

EMMA comprises over 6000 mutant mouse lines carrying targeted, transgenic, endonuclease - mediated, induced and other types of mutations.

The phenotyping pipeline that will be used within INFRAFRONTIER - I3 builds on the EUMODIC pipeline and uses a cohort of seven female and seven male mutant mice for a comprehensive first - line phenotyping analysis.

Through special agreements with originators, the MMRRC distributes mutant ES cell clones, parental and feeder cell lines.

Such cell lines are now opening the opportunity to carry out «large scale» functional genomics screens in order to identify the genes able to revert the mutant phenotype of the cells.

The recessive male - sterile gene contained in the IR36 mutant and the dominant male - sterile gene contained in PXDMS will each be transferred into five parental lines.

A rice mutant population was generated by fast neutron bombardment of the rice line Kitaake - Ubi - Xa21.

[21] In the recently presented FLAURA study, in which osimertinib was used in untreated patients with advanced EGFR - mutant NSCLC, the HR for systemic disease control and CNS control similarly favored osimertinib over erlotinib or gefitinib, supporting the preclinical data that showed osimertinib's penetration across the BBB and providing support for using this agent in first - line management of EGFR - mutant patients with brain metastases.

Intermediates that were quantified in wild - type and bsl1 - 1 mutants are boxed by a dotted line.

Both mutants were crossed to the parental A10.1 line, and the resulting progeny of the selfed F1 individuals displayed the expected Mendelian 3:1 ratio for a single locus recessive allele (31:16; P [χ2, 1 df] = 0.15 and 40:10; P [χ2, 1 df] = 0.41], respectively).

However, using these cell lines it should be possible to determine in detail how the defects arise (which is difficult in patients), and perhaps how to alter the segregation of mutant versus normal mtDNA during cell division to eventually lessen the burden of abnormal mitochondria function.

They show that it is possible to derive iPS cells from a patient who has a mix of mutant and normal mtDNA (is heteroplasmic), where the separate cell lines can have anything from 0 to 100 % mutant mtDNA, but the same nuclear DNA.

One mutant produced few to no bristles while the other displayed a weaker phenotype, producing substantially fewer and shorter bristles than the A10.1 reference line (Supplemental Table 1 and Supplemental Figures 1B and 1C).

GMC strategy: To develop and offer a large scale standardised and comprehensive phenotypic analysis of mouse mutants from various sources (e.g. transgenic lines, knockout mice, ENU mutants or endonuclease mediated mutations)

The technical evaluation of projects may require the provision of additional data such as information on the genetic modification of your mutant mouse line if applicable (e.g. affected gene, MGI ID of the gene, type of mutation, ES - cell line used, genetic background (e.g. number of backcross generations), safety level, description of DNA modification, vector, remaining non-recipient DNA, donor organism), mutant phenotype (s), special housing or care requirements, current sanitary status, and intellectual property rights (who generated the mouse line, owner of the mouse line)

Test each mutant mouse line (4,000 mouse lines in the first 5 years, and ultimately up to 20,000) through a broad based primary phenotyping pipeline in all the major adult organ systems and most areas of major human diseases.Through this activity and employing data annotation tools, systematically aim to discover and ascribe biological function to each gene, driving new ideas and underpinning future research into biological systems.

Using this line, we explored whether the gbp1, gbp2 ex67 mutant shows phenotypes similar to GBP1, GBP2 double knockdown animals.

As noted in the JAX ® Mice strain data sheets, the best control for a targeted mutant is the 129 strain used to produce the ES cell line from which the mutant was derived (Simpson et al. 1997; Threadgill et al. 1997).

We created the gbp1, gbp2 ex67 mutant by a P - element excision mutagenesis method using the P - element insertion line, y [1] w [*]; P -LCB- w [+ mC] = EP -RCB- G19340 (Bloomington Drosophila Stock Center # 28111).

On the other hand, a Brs3 agonist promoted insulin secretion in both rodent insulinoma cell lines and in islets isolated from wild - type but not Brs3 mutants [53].

In our laboratory, we have previously described an in vitro model of spontaneous transformation of the IL -3-dependent hematopoietic BaF3 cell line towards growth factor - independent tumorigenic clones with constitutive STAT5 activation.14 This model was developed from the study of BaF3 cells transfected with an IL - 9Rα mutant lacking the STAT - recruiting site (BaF3 phe116).

Use of Illumina sequencing to identify transposon insertions underlying mutant phenotypes in high - copy Mutator lines of maize

The phenotypes were identical among the mutant mice from two independent lines and we did not distinguish our analyses between the two lines.

Here we show the results of screening 810 mutant mouse lines using an in vivo assay to identify microenvironmental regulators of metastatic colonization.

Note: For live mice from a cryo - preserved line, you are ordering at minimum the Resuscition; a quantity of 1 litter is normal as you will receive the resulting litter with at least one mutant carrier for breeding.

Mutant mouse and cell lines are distributed using the MMRRC Conditions of Use * (COU).

A mutant cell line defective in response to double - stranded RNA and in regulating basal expression of interferon - stimulated genes

Determination of the expression pattern of antibody - or GFP - labelled puncta (presumptive UNC -7-containing gap junctions) in various mutant backgrounds was based on examination of at least two (typically more) independently isolated transgenic lines for each condition.

Using this, we have identified abnormalities in these structures among 298 embryos from mutant mouse lines carrying embryonic lethal gene mutations produced for the Deciphering the Mechanisms of Developmental Disorders (DMDD) programme.