Sentences with phrase «neo-apc expression vector»

Cloning in RNA expression vectors was combined with a sensitive electrophysiological assay in Xenopus oocytes in order to isolate a functional cDNA clone encoding the 5HTlc serotonin receptor.

When this clone was inserted into a mammalian expression vector and transfected into COS - 7 cells, a protein that specifically phosphorylated the agonist - occupied form of the beta 2 - adrenergic receptor and phosphorylated, much more weakly, the light - bleached form of rhodopsin was expressed.

They inserted the genetic sequence that codes for the p55 receptor into a loop of DNA called an expression vector.

To examine the effect of miR - 7 on the expression of KLF4, we ectopically expressed miR -7-1 or miR -7-2 in 231BoM and 231BrM cells by infecting corresponding lentivirus expression vector followed by qRT - PCR analysis.

DNA from the individual VHH that neutralized any tier 2/3 viruses to < 20 % seen with control was purified, sequenced, and recloned into the pCAD51 expression vector followed by transformation into TG1 cells for purification and further characterization.

Lentiviral expression vectors are the most effective vehicles for delivering genetic material to almost any mammalian cell

Clade B recombinant Nef protein, expressed as a His - tag fusion protein in E. coli using expression vector pET24 and purified using Ni - agarose columns.

Clade C recombinant p17 / 24 - B protein, expressed as His - tag fusion protein in E.coli using expression vector pET24 and purified using Talon Superflow metal affinity resin.

May be used for the affinity purification of the SIV - Pk fusion protein from bacterial expression vectors 282 and 283.

Clade B recombinant p17 / 24 - B protein is expressed as His - tag fusion protein in E.coli using expression vector pET24 and purified using Talon Superflow metal affinity resin.

The rev cDNA from pCV - 1 (NIH Cat # 303) was amplified using PCR and cloned into the expression vector pOTS Nco1 (SmithKline Beecham).

Amphotrophic virus stocks were prepared by co-transfecting 293T cells with the resulting pBABE - puro construct, a VSV - G envelope expression vector, and pSV - gag - pol.

Insert for human APOBEC3G was cloned into mammalian expression vector pcDNA3 with a V5 epitope tag from SV5 paramyxovirus and a polyhistidine epitope at the C terminus.

Recombinant TAT protein is expressed as His tag - fusion protein in E.coli using expression vector pET24.

Clade C recombinant nef protein, expressed as His - tag fusion protein in E. coli using expression vector pET24 and purified using Ni - agarose columns.

Recombinant SIVmac Clone J5 rev protein is expressed as His tag - fusion protein in E.coli using expression vector pET24.

Recombinant RT protein from SIVmac clone J5 protein is expressed as His tag - fusion protein in E.coli using expression vector pET24.

Clade A recombinant nef - protein, expressed as His - tag fusion protein in E. Coli using expression vector pET24 and purified using Ni - agarose columns

The bacterial expression vector pGEX - 5 × -1 / Sp1 was constructed by introducing an EcoRI / XhoI — treated Sp1 gene fragment into EcoRI / XhoI — treated pGEX - 5 × -1 vector.

The mammalian expression vector pcDNA - SRα / FLAG - DDX 3 was constructed by insertion of a 2 - kb EcoRI / ApaI — treated fragment of the DDX3 gene derived by reverse transcription - PCR (RT - PCR) into EcoRI / ApaI — treated pcDNA - SRα / FLAG vector.

Some highlights of the 2nd edition eBook include: new chapters on genome engineering, cloning, and plasmid tags, addtional content added to the «Plasmids that Glow» and «Viral Expression Vectors» chapters, and more pictures and pro tips.

The authors first stably transduced freshly derived multipotent early passage hDPSCs with a PDGF - BB expression vector, generating cells that displayed heightened proliferation when compared to control hDPSCs.

Approaches such as the baculovirus expression vector system (BEVS) in use at uniQure (Amsterdam, The Netherlands) for manufacturing alipogene tiparvovec may provide suitable options (4).

The two PCR reactions are then mixed and transfected along with a Cas9 expression vector.

Selected Amrose variant fluorescent proteins were subcloned into the pCS2 + expression vector [32](EcoRI - XbaI) using the gel purified amplicons generated above.

The expression vector contained the cDNAs coding for a potential fusion protein containing the GFP (EGFP), the 2A esterase of foot - and - mouth disease virus (FMDV), and HOXB4 with an additional N - terminal hemagglutinin (HA) epitope tag (eGFP2AHOXB4).

A translational fusion of Punc - 7S in the GFP expression vector pPD95.67 (provided by A Fire) was injected at a concentration of 50 ng / μl and found to express in various head neurons but not in ventral nerve cord motor neurons.

Adenovirus was produced by transfecting adenoviral expression vector into E1 - complemented cell line 293A and performing successive rounds of amplification until high titer was achieved.

iBET know - how in terms of biopharmaceuticals spans from the initial expression vector design and cell line establishment through all stages of process development and scale - up (production, purification, stability and storage).

In some experiments, 4 ng expression vector was included.

As a test of this model, that these potential Oct4 phosphorylation events can shape the ES cell transcriptional landscape, Oct4 point mutants in an inducible expression vector can be stably transfected into ES lines which conditionally express wild - type Oct4 [14].

The bacterial and mammalian expression vectors for these CRISPR inhibitors are listed in the table below.

(B) Schematic representation of the pCMV - Neo-APC expression vector that codes the full - length APC sequence together with wild - type or mutated miR - 142 targeted site located at the 3 ′ UTR.

Modified genes, expression vectors, probes, modified proteins, monoclonal antibodies and receptors, cells and other chemical compounds such as small organic molecules are patentable.

Tags for this Online Resume: Lentiviral Retroviral shRNA RNA knockdown screens, Flow Cytometry, RTPCR, Immunopreciptations, Reporter assays, Cell based Assays, ELISA, Migration, Stable and Transient Transfections, Genomic and Proteomic Studies, Cloning, Transient siRNA knockdowns, Phenotyping Mammalian cell lines, Gene Overexpression, Subcloning to expression Vectors, Construct Stable Inducible Cell lines, Protein Kinase Assay, Pulse Chase Experiments, Transfection, Transduction, Transformation, Polyoma virus DNA tumor virus replication, Research Scientist, Cell Biology Protein Biochemistry Genetics Molecular Bioliogy Oncology Virology, FACS

Not exact matches

If in a mathematics GCSE examination, a student lost 13 marks on ratio & proportion (D / C grades), simplifying expressions and index laws (E-B grades) and also lost 9 marks on transforming graphs (A * grade) and vectors (A * grade).

Germline Transmission and Tissue - Specific Expression of Transgenes Delivered by Lentiviral Vectors

Using support vector machine recursive feature elimination, the researchers identified three gene expression signatures that predicted therapy responses.

In addition, four tRFs had differential expression patterns after feeding with dengue virus and three had altered levels after treatment with antibiotics, suggesting a role in gut microbiome and vector competence to dengue virus infection.

Overexpression of wild - type ACVR1 in chick micromass cultures resulted in expression levels of marker genes that are comparable to those of the empty viral vector, while cells overexpressing R206H ACVR1 showed upregulation of collagen type II and aggrecan.

Remarkably, the relative expression levels of all marker genes were only slightly decreased when R206H ACVR1 and Q207D caACVR1 cultures were cotransfected with Noggin, whereas cells expressing the wild - type ACVR1 or empty viral vector showed clear downregulation of all analyzed marker genes for chondrogenesis.

The vector is a self - inactivating lentivirus — a member of a family of viruses that can insert genes into mammalian cells and drive expression of those genes — that has been engineered to avoid triggering the development of leukemia, a complication seen in previous gene therapy trials for immunodeficiency syndromes, including WAS.

While expression levels of all marker genes remained almost unchanged in wild - type ACVR1 cultures compared with the empty viral vector, the expression profile induced by caACVR1 Q207D displayed evidence of advanced chondrogenic differentiation as shown by decreased levels of the early differentiation stage marker collagen type II together with enhanced expression of the later - stage markers Ihh and collagen type X. Indian hedgehog has been shown previously to be induced by BMP signaling transmitted via the ACVR1 receptor (9).

OMC also aims at potentiating the existing Conditional Mutagenesis Facility by implementing novel approaches to attain tissue -, stage - and site - specific transgene expression in mouse mutant strains, mainly through lentiviral vector - mediated transduction of blastocysts.

Persistence of non-viral vector mediated RPE65 expression: Case for viability as a gene transfer therapy for RPE - based diseases.

TRANSVAC offers a diverse range of expression systems including diverse vectors, regulatory elements, and cloning strategies (as described under services).

Svensson, Eric et al. «Long - term erythropoietin expression in rodents and non-human primates following intramuscular injection of a replication - defective adenoviral vector.»

BTHP - 1 parental cells were transduced with the MLV vector MX - DC - SIGN and FACS sorted as a population for high levels of DC - SIGN expression.

The therapy hoped to resolve some of the problems surrounding haemophilia treatment by exchanging the continual infusions for a single vector infusion that could permanently boost Factor IX expression.

As the level of gene activity knockdown associated with transgenic RNAi depends on the level of expression of the hairpin constructs, we generated a number of derivatives of our initial vector, called the «VALIUM» series, to improve the efficiency of the method.