Sentences with phrase «neubauer hemocytometer»
Beginning 72 hours post-stimulation, cells were counted every 48 hours using trypan blue dye exclusion on an automated hemocytometer (Countess, Invitrogen) and split to 0.8 × 106 with fresh media containing 100 U / ml IL - 2.
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SVCs isolated from adipose tissue samples were cooled on ice and counted using a hemocytometer.
For the colony formation assay, cells were trypsinized to generate single - cell suspensions and counted by a hemocytometer.
At each 24 - hour interval, the cells were collected, stained with trypan blue, and counted with a hemocytometer.
Cells were dissociated by trituration, counted on a hemocytometer and resuspended in conditioned medium at 1000 cells / µl.
The plated cells were then dissociated and counted using a hemocytometer in order to express results as picograms or nanograms of growth factor produced per day per million cells.
At the end of this incubation, all rafts were thoroughly washed with PBS to remove any non-adherent cells, while adherent cells were trypsinized and counted using a hemocytometer.
* Lab Instruments: Oscilloscope, Function generator, digital multimeter, Basic circuit elements, spectrophotometer, microscope, flow cytometer, hemocytometer, ventilators.