In just a little over a year of operation, Ames Laboratory's dynamic
nuclear polarization (DNP) solid - state nuclear magnetic resonance (NMR) spectrometer has successfully characterized materials at the atomic scale level with more speed and precision than ever possible before.
In the past decades, dynamical
nuclear polarization (DNP) has been utilized to get orders of magnitude enhancements of nuclear magnetic resonance signals.
On the other hand, dynamic
nuclear polarization of molecules via nitrogen vacancy centers has important applications in nuclear magnetic resonance spectroscopy since it would greatly increase the standard sensitivity of current scanners.
«Dynamic
nuclear polarization has a capacity to transform our understanding of biological structures in their native contexts,» says Whitehead Institute Member Susan Lindquist, who is also a professor of biology at MIT and one of the senior authors of the paper, which appears in the October 8 issue of Cell.
Using this type of NMR, which is based on a technique known as dynamic
nuclear polarization (DNP), scientists can gain much more insight into protein structure and function than they can with current NMR technology, which requires large quantities of purified proteins, isolated from their usual environment.
«We envision highly enhanced NMR of liquids and solids using existing polarization transfer techniques, such as cross-polarization in solids and cross-relaxation in liquids, or direct dynamic
nuclear polarization to outside nuclei from NV centers,» King says, noting that such transfer of polarization to solid surface and liquids had been previously demonstrated by the Pines group using laser polarized Xe - 129.
«Also, in our previous studies, we inferred the presence of
nuclear polarization indirectly through optical measurements because we weren't able to test if the bulk sample was polarized or just the nuclei that were very close to the NV centers.
The researchers were puzzled by a nonlinear drop in surface water diffusivity — as measured by the Overhauser dynamic
nuclear polarization apparatus in the Han lab — as the chemical composition of the silica surface moved from hydrophobic to hydrophilic.
Not exact matches
The former is ideal for soft - tissue contrast, and the latter has extremely fine imaging resolution due to a revolution in the technology called dynamic
nuclear spin
polarization, which is used to track minute biochemistry in the body — such as the transition of the naturally occurring chemical pyruvate to lactate.
NMR / MRI signals depend upon a majority of
nuclear spins being polarized to point in one direction — the greater the
polarization, the stronger the signal.
The authors report the observation of a bulk
nuclear spin
polarization of six - percent, which is an NMR signal enhancement of approximately 170,000 times over thermal equilibrium.
Resonant microwave radiation can then be used to transfer this
polarization to surrounding
nuclear spins.
Moreover, we show that using the integrated solid effect both for single - and double - quantum transitions
nuclear spin
polarization can be achieved even when the static magnetic field is not aligned along the NV's crystal axis.
The tiny thermal
nuclear spin
polarization represents a major obstacle towards this goal which may be overcome by dynamic
nuclear spin
polarization (DNP) methods.
This high levels of hyperpolarization, together with the long
nuclear - spin
polarization lifetimes in nanodiamonds and the relatively high density of 13C nuclei, turn functionalized and hyperpolarized nanodiamonds into attractive MRI probes for molecular imaging both in vitro and in vivo.