The third is a method implemented by Reich for
reading the genetic codes of 1.2 million carefully chosen variable parts of DNA (known as single
nucleotide polymorphisms) rather than having to sequence entire genomes.
In this project, I applied NASP for single
nucleotide polymorphism (SNP) identification and characterization, and used FigTree and PHYLOBar for phylogenetic analysis and visualization.The NASP method differs from other published SNP pipelines in terms of supported short -
read aligners and SNP callers, the ability to call both monomorphic and polymorphic sites, and the ability to integrate the results from multiple SNP callers and identify the consensus set of SNPs that define the population structure.