Sentences with phrase «ob rats»

WNIN Mutant Obese Rats Develop Acute Pancreatitis With the Enhanced Inflammatory Milieu WNIN Mutant Obese Rats Develop Acute Pancreatitis With the Enhanced Inflammatory Milieu WNIN / Gr - Ob rats demonstrate features of metabolic syndrome that include obesity, insulin resistance, impaired glucose tolerance, and hyperinsulinemia.

WNIN / Gr - Ob rats demonstrate features of metabolic syndrome that include obesity, insulin resistance, impaired glucose tolerance, and hyperinsulinemia.

Our previous studies have also reported increased episodes of obesity - associated neurodegeneration, cataract, cancer, infertility and immune dysfunction and accelerated aging in WNIN / Gr - Ob rats [30].

L - arginine administration altered insulin secretion in both control and WNIN / Gr - Ob rats.

Increased expression of Cd11b - a marker of macrophages [38] seen with L - arginine admininistration in WNIN / Gr - Ob rats, might be due to increased influx of inflammatory cells in the exocrine fraction via adhesion molecules [17].

A significant decrease in SOD levels (Fig. 3b) and catalase (Fig. 3c) was demonstrated in WNIN / Gr - Ob rats with L - arginine treatment.

L - arginine treatment induced increased expression of IL - 6 and IL - 1β (immunohistochemistry) in islets of WNIN / Gr - Ob rats compared to controls rats.

In par with these studies, the propensity to increase the levels of inflammatory cytokines (IL - 6 and IL - 1β) expression in islets of WNIN / Gr - Ob rats were exacerbated with L - arginine administration in mutants.

These WNIN / Gr - Ob rats also portray accelerated aging, with increased episodes of obesity - associated neuro - degeneration, cataract, cancer, infection and infertility at advanced age [13].

Administration of larginine increased acinar cells in G1 phase in both WNIN / Gr - Ob rats and control rats.

Increased systemic inflammation and islet dysfunction were also observed in WNIN / Gr - Ob rats as compared to control when L - arginine was administered.

Conclusion: We advocate WNIN / Gr - Ob rats as a novel model system to study the pathophysiology of severe acute pancreatitis associated with obesity.

Methods: We evaluated the outcome of L - arginine - induced acute pancreatitis, in WNIN / Gr - Ob rats as compared to control rats.

This concentration was selected as increasing concentration resulted in increased mortality in WNIN / Gr - Ob rats.

WNIN / Gr - Ob as well as its parental controls were randomly allocated into four groups (six rats in each group)(Fig. 1): group 1: parental control rats treated with saline; group 2: parental control rats treated with L - arginine; group 3: WNIN / Gr - Ob rats administerd with saline; and group 4: WNIN / Gr - Ob rats treated with L - arginine.

Therefore, decrease in activity of these enzymes in WNIN / Gr - Ob rats at basal conditions, may be a primary reason for the acceleration of the pancreatic injury presently observed with L - arginine induction (Fig. 8).

Our previous studies have demonstrated increased inflammation in adipose, pancreatic tissue and mesenchymal stem cells isolated from bone marrow of WNIN / GR - Ob rats [14 - 16].

Interestingly, we have earlier reported for the predominance of the inflammatory milieu of the pancreas [17, 18], adipose [35] and bone marrow in WNIN / Gr - Ob rats [14] and probably this may be an important confounding factor to predispose WNIN / Gr - Ob rats toward development of pancreatitis, as compared to their age - matched parental control rats [17].

Based on these findings, we hypothesize that WNIN / Gr - Ob rats with their inherent phenotypic features such as obesity, IR and IGT vis - a-vis pre-inflammatory milieu in situ, would form the feasible model system to portray for the pathophysiology of AP induced by L - arginine.

In agreement, our published data from genome - wide expression from the pancreas of WNIN / Gr - Ob rats further confirm the pre-dominance of ER stress, as the major inflammatory response underlying in WNIN / Gr - Ob rats [17].

We also have earlier reported for an impaired response in WNIN / Gr - Ob rats at basal condition [18].

We observed increased TBARS (Table 1) and ROS levels in the pancreatic tissues of WNIN / Gr - Ob rats in both basal and with L - arginine induction as compared to their respective controls (Fig. 3a), which is in line with previous findings [18].

L - arginine treatment resulted in an increase of all these proteins in cells from WNIN as well as WNIN / Gr - Ob rats, but a higher increase was detected in cells from WNIN / Gr - Ob rats compared with those from WNIN rats (Fig. 5).

Background: WNIN / Gr - Ob rats demonstrate features of metabolic syndrome that include obesity, insulin resistance, impaired glucose tolerance, and hyperinsulinemia.

However, the levels were significantly higher in WNIN / Gr - Ob rats as compared to the controls (Table 1).

Further, L - arginine administration increased vacuoalation in hepatic tissue of WNIN / Gr - Ob as compared to control rats (Fig. 2d), indicating more severe liver injury in WNIN / Gr - Ob rats [27].

Even at the basal conditions, higher levels of IL - 1β and TBARS in WNIN / Gr - Ob rats were seen as compared to control rats (Table 1 and Fig. 4b).

Further studies are needed to gain an insight to delineate the endocrine - exocrine cross-talk underlying inflammation in WNIN / Gr - Ob rats.

Not exact matches

Manuscript submitted May 10, 2017, accepted June 15, 2017 Short title: Pancreatic Stress in WNIN / GR - Ob Mutant Rats doi: https://doi.org/10.14740/cmmr11e

However, the heterogeneity was higher in L - arginine administered WNIN / Gr - Ob as compared to WNIN rats treated with L - arginine (Fig. 2b).

These changes were profound in WNIN / Gr - Ob with L - arginine administration as compared to control rats (Table 1).

However, no significant difference was found in amylase and lipase levels in WNIN / Gr - Ob and control rats at basal conditions (Table 1).

At the cellular level, L - arginine administration resulted in acinar cell necrosis in both WNIN / Gr - Ob and control rats.

L - arginine administration induced AP in WNIN / Gr - Ob and control rats as demonstrated by an increase in serum lipase and amylase levels.

In line with our hypothesis, L - arginine treatment caused significantly higher alterations in histopathological / biochemical parameters in WNIN / Gr - Ob as compared to their parental control rats.