Sentences with phrase «oligo dt»

RNA was DNase treated (Roche) and cDNA was reverse transcribed using Transcriptor (Roche) with oligo dT as the primer.

After DNase treatment, total RNA concentration was quantified and 1 μg total RNA was converted to cDNA using oligo dT and reverse transcriptase.

Not exact matches

And we can only sell twelve of them a year because there's not enough oligos in the world.

He brought it into Illumina, got it working, licensed it out to the oligo manufacturers, brought the price of oligos down by like 90 % and dramatically increased supply» — which enabled the now — $ 24 billion - in - market cap Illumina to succeed.

The hope was that antisense oligos for everything from cancer to HIV would roll off the pharmaceutical assembly line — but despite a generation of valiant effort, the successes on this front have been meagThe hope was that antisense oligos for everything from cancer to HIV would roll off the pharmaceutical assembly line — but despite a generation of valiant effort, the successes on this front have been meagthe pharmaceutical assembly line — but despite a generation of valiant effort, the successes on this front have been meagthe successes on this front have been meager.

(FODMAP is an acronym for fermentable oligo - di - monosaccharides and polyols, which is the name of the carbohydrate in question.)

About Sisterna... Sisterna B.V. is the market leader in high - grade sucrose esters and sucrose oligo esters.

Sometimes lactobacilli need a bit of help getting hold in the intestines, and some practitioners recommend FOS (fructo - oligo - saccharides) to enhance colonization.

In a new study, patients who ate rye bread that was low in so - called «FODMAPs» (fermentable oligo - di - and mono - saccharides and polyols) experienced milder IBS symptoms than patients who ate normal rye bread.

After tinkering with several ingredients, they struck gold with a mixture of two chemicals, oligo (e-caprolactone) dimethacrylate and n - butyl acrylate.

A new Oligo - Miocene marsupial lion from Australia and revision of the family Thylacoleonidae.

Levels of APOE protein dropped by about half in mice given the APOE compound as compared with those that received the placebo oligo or saltwater.

The mice that received the APOE antisense oligos had about half as many amyloid plaques as mice given saltwatThe mice that received the APOE antisense oligos had about half as many amyloid plaques as mice given saltwatthe APOE antisense oligos had about half as many amyloid plaques as mice given saltwater.

Each molecular group includes tumors from all grades and categories of astrocytoma, oligodendrocytoma and oligo - astrocytoma

We put our starting molecule» — a structure called oligo - enediyne, composed of three benzene rings linked by carbon atoms — «on a silver surface, and then induced reactions by heating it.»

I use one of the fancy pipettes to move the oligo into the plasmid tube, and I also add buffer, water, and an enzyme.

The oligo arrives, and I lose my modern pipetting virginity.

If all goes well, the enzyme will cut open the plasmid, removing a piece of its DNA and allowing the oligo to take its place.

My task is to pipette the oligo from one tiny test tube into another.

Wagner conducts the experiment in parallel with me, and his plasmid properly incorporates the oligo that will guide Cas9 to its target.

After four rounds of selection, the enriched oligo pool was sequenced, and a position weighted matrix was generated for the 12 bp target site and one flanking residue per side (faded).

MyOPGP data has facilitated the development of important genomic platforms, such as single nucleotide polymorphism (SNP), oligo and methylation arrays.

cDNA synthesized by reverse transcription of 1 µg RNA primed with oligo (dT) and random 9 - mer primers was used as the substrate for quantitation of mRNA expression levels by quantitative RT — PCR in the presence of SYBR ® Green (Stratagene, La Jolla, CA).

cDNA was prepared from total RNA by reverse transcription using standard protocols for the SuperScript ® III First - Strand Synthesis System (Invitrogen) and oligo (dT) priming.

Specifically, reverse transcription of 5 µg total RNA primed with an oligo (dT) primer bearing a T7 promoter was followed by in vitro transcription, generating multiple antisense copies of each mRNA in the sample (Ambion, Austin, TX).

Oligo - astheno - teratozoospermia in mice lacking ORP4, a sterol - binding protein in the OSBP - related protein family.

Here we utilized BD ™ AbSeq, a novel protein sequencing approach enabled by oligo - conjugated antibodies, for simultaneous detection of mRNA and protein in single cells.

We computed Robust Multichip Average expression measures using the Oligo package in Bioconductor (22).

The antibody - specific oligos were captured, amplified, and sequenced alongside mRNAs in a single workflow on the BD RhapsodyTM single - cell analysis systThe antibody - specific oligos were captured, amplified, and sequenced alongside mRNAs in a single workflow on the BD RhapsodyTM single - cell analysis systthe BD RhapsodyTM single - cell analysis system.

gRNAs targeting every gene in the human genome for example, can be made easily and cheaply using oligo library synthesis.

With SapTrap, the user first designs either oligos or synthetic DNA for the desired gRNA target sequence, as well as the 5» and 3» homology arm repair template (Fig. 1, Step 1).

RNA was isolated from cancer cells using the RNeasy RNA isolation kit (Qiagen), and complementary DNA was synthesized using oligo (dT) primers and Moloney murine leukemia virus reverse transcriptase (Invitrogen).

The primers in the KAPA kit target Illumina ® P5 and P7 flow cell adapter oligo sequencThe primers in the KAPA kit target Illumina ® P5 and P7 flow cell adapter oligo sequencthe KAPA kit target Illumina ® P5 and P7 flow cell adapter oligo sequences.

Importantly, this requires a separate assay: usually, a custom array with oligos designed for each exon in every panel gene.

The oligo probes are in situ synthesis (Affymetrix 25mer) or spotted (custom ncRNA array 40mer) and immobilized on the chThe oligo probes are in situ synthesis (Affymetrix 25mer) or spotted (custom ncRNA array 40mer) and immobilized on the chthe chip.

Reverse transcription was performed on 1 μg of total RNA with an oligo (dT) primer (Roche) and M - MLV RT (Invitrogen).

The data collected by microarray are based on the fluorescence signal intensities (analogue data) from known sequenced oligo probThe data collected by microarray are based on the fluorescence signal intensities (analogue data) from known sequenced oligo probthe fluorescence signal intensities (analogue data) from known sequenced oligo probes.

A pair of oligos for each targeting site (Table S1) were annealed and ligated into linearized pX330 vector for generating gRNA - expressing plasmid.

To further prove the specificity of the binding of STAT6 to the COX - 2 promoter sequence, electrophoretic mobility shift assays (EMSA) were performed utilizing a 30 bp oligo from the COX - 2 promoter sequence encompassing the STAT6 binding site as a probe.

The microarrays utilized in the ncRNA Program are oligo - based Affymetrix and in - house custom arraThe microarrays utilized in the ncRNA Program are oligo - based Affymetrix and in - house custom arrathe ncRNA Program are oligo - based Affymetrix and in - house custom arrays.

The Glycolytic Versatility of Bacteroides uniformis CECT 7771 and Its Genome Response to Oligo and Polysaccharides

Orders must be submitted using the Oligo Ordering Form.

For droplet based data one simple reason for the heterogeneity could be due to variation in coverage for DNA oligos on barcoded beads.

FODMAPS is an acronym for the unwieldy phrase: fermentable oligo -, di -, and monosaccharides and polyols (sugar alcohols).

Most likely symptoms of «gluten sensitivity» are actually caused by an herbicide (weed - killer) and a group of carbohydrates known as fermentable oligo - di - monosaccharides and polyols, or FODMAPs for short.

A low - FODMAP (Fermentable, Oligo -, Di -, Mono - saccharides, and Polyols) diet can also help short term.

And we may even go to a low FODMAP diet where we cut out the fermentable carbohydrates — fermentable oligo - di - po — let's see, fermentable oligo - di - mono and polysaccharides.

They reiterate that all of the following foods can precipate digestive symptoms in IBS patients: dairy, foods which are high in FODMAPS (fermentable oligo -, di -, monosaccharides, and polyols), high fat and spicy foods, foods with high levels of biogenic amines, i.e. histamine (such as soy), lectins (present in beans), and preservatives, such as benzoic acid and sulfites.

These carbohydrates are part of the FODMAPs (acronym means: Fermentable Oligo -, Di -, and Mono - saccharides And Polyols).

Even FODMAP approaches or specific carbohydrate approaches where we cut down these Fermentable, Oligo -, Di -, Mono - saccharides and Polyols — the specific fermentable sugars in the diet.