Sentences with phrase «osteoclasts from»

Osteoclasts from mice that lacked Del - 1 or from cell lines lacking the protein could be differentiated faster and stronger.

Not exact matches

We added the optimal dose of IL - 6 required to promote maturation of osteoclasts (Fig. 5I) to conditioned medium derived from ABL1 / ABL2 knockdown breast cancer cells.

Through its various targets, MMP1 promotes not only tumor invasion but also breast cancer colonization to bone by mechanisms that include the release of membrane - bound EGF - like growth factors from tumor cells, leading to activation of EGF receptor signaling and suppression of OPG expression in osteoblasts, which in turn promotes the differentiation and activation of osteoclasts required for bone destruction and enhanced tumor growth in the bone microenvironment (32).

To evaluate whether ABL kinases might regulate the secretion of osteoblast - derived RANKL or OPG leading to osteoclast differentiation, we analyzed RANKL and OPG mRNA abundance in the murine osteoblast cell line 7F2 in response to conditioned medium from control and ABL1 / ABL2 knockdown breast cancer cells.

Addition of IL - 6 to reconstituted conditioned medium from ABL1 / ABL2 - depleted breast cancer cells partially restored osteoclast activation (Fig. 5, J and K).