The following
primary antibodies were used: Oct - 4 (19857 Abcam) 1 ∶ 1000; β - III tubulin clone SDL.3 D10 (T8660 Sigma) 1 ∶ 500; Nestin (611658 BD Transduction laboratories) 1 ∶ 500 and TH (P40101
Pel - Freez) 1 ∶ 500, and as secondary antibodies: Alexa Fluor 594 Goat Anti-Mouse, Alexa Fluor 488 Goat Anti-Rabbit, Alexa Fluor 594 Goat Anti-Rabbit.