Sentences with phrase «phosphate buffer saline»

The sections were treated with 3 % H2O2 in phosphate buffer saline (PBS), then incubated with serum blocking reagent for 30 minutes.

Samples for histology were collected as for microbial analysis (see above), with the same sample number of samples however, they were preserved in 5 % paraformaldehyde made up with Phosphate Buffer Saline (PBS).

The doubling time of the cell line was 21 hours.33 At confluence, cells were harvested with 0.25 % trypsin and then were re-suspended at 1,5 × 106 cells in 0.15 ml PBS (Phosphate Buffered Saline, Dulbecco, Biochrom) which was injected in mice.

Polyclonal antisera from immunized hosts are lipid extracted to improve clarity, salt fractionated, dialyzed against phosphate buffered saline containing sodium azide, and freeze - dried.

Mice were sacrificed between 5 days and 20 weeks post infection by transcardial perfusion with 20 mL ice - cold sterile phosphate buffered saline following anesthesia with 500 µL 2.5 % avertin administered intraperitoneally (i.p.).

Cells were washed three times with ice - cold 1X PBS, scraped into 1 ml of phosphate buffered saline (PBS) containing protease inhibitors (1x G - Biosciences Protease Arrest, 200 µM Na3VO4, and 1 mM PMSF) and collected by centrifugation (700xg for 4 min).

The plates were blocked with 2 % Marvel milk powder in phosphate buffered saline (PBS) for 2 h at 37 °C, washed three times with PBS, 0.05 % Tween 20.

The skin biopsies were washed in Ca / Mg - free Dulbecco's Phosphate Buffered Saline (PBS, Invitrogen, Carlsbad, CA, http://www.invitrogen.com) and minced into approximately 12 smaller pieces before being seeded onto gelatin - coated 6 - well cell culture plates (Corning Enterprises, Corning, NY, http://www.corning.com) containing mouse embryonic fibroblast (MEF) media consisting of Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10 % fetal bovine serum (FBS, Invitrogen) and 100 IU / ml penicillin - streptomycin (Invitrogen), and cultured at 37 °C in a 5 % CO2 incubator.

All antibodies were diluted in phosphate buffered saline containing 0.05 % Tween (PBS - T).

The primary antibody for p53 (see Table 1) was diluted 1:500 in phosphate buffered saline (PBS).

Afferents to the visual wulst were mapped by stereotactic iontophoretic application (4 µA positive current, 7 sec on / off, duration: 20 — 30 min) of biotinylated dextran amin (BDA, working dilution: 10 % in phosphate buffered saline, PBS; Molecular Probes Europe BV, Leiden, The Netherlands) into Cluster N or into medial parts of the visual Wulst.

At the end of the experiment, birds were killed by an overdose of Narcoren (Merial, Hallbergmoos, Germany) and transcardially perfused with 0,12 M phosphate buffered saline (PBS) followed by 4 % paraformaldehyde (PFA) dissolved in PBS.

The rats were first treated with kainic acid and phosphate buffered saline (PBS), both of which are substances which scientists use to deliberately increase free radicals so they can then test treatments for oxidative stress.

Mononuclear cell pellets were separated from the diluted blood specimen by centrifugation with ficoll (400 g, 30 min) and the mononuclear cell layer was removed from the tube using a transfer pipette, resuspended in a phosphate buffered saline solution, and briefly centrifuged again.

Not exact matches

Sections were incubated in 0.3 % hydrogen peroxide in PBS for 20 min, washed in PBS - T (0.01 M phosphate - buffered saline, 0.2 % Triton X-100) and blocked 1 h with 10 % normal goat serum (NGS) in PBS - T.

Non-confluent cultures were trypsinized into single - cell suspension, counted, washed with phosphate - buffered saline (PBS), and fixed with 10 % paraformaldehyde.

For intracardiac injections, cells were harvested from subconfluent culture plates, washed with phosphate - buffered saline (PBS), and resuspended at 106 / ml (1833) or 5 × 106 / ml (SCP28) in PBS; 0.1 ml of the suspended cells was injected into the left cardiac ventricle using 30 - gauge needles.

Brain or ovary tissues from the affected ducks were homogenized in sterile phosphate - buffered saline (PBS, pH 7.2) to give a 20 % suspension (w / v).

Cells were washed in phosphate - buffered saline (PBS) containing calcium and magnesium, fixed in 4 % paraformaldehyde in PBS for 10 minutes, washed twice with PBS, and then permeabilized with 0.2 % Triton - X100 in PBS for 5 minutes.

The colonies were fixed in culture dishes with 4 % paraformaldehyde in phosphate - buffered saline (0.01 M PBS, pH 7.4) for 20 min at room temperature (RT) followed by washing with PBS (2 × 5 min).

Cell pellets were resuspended in phosphate - buffered saline, total cell count was performed, and then cytospins were prepared with 5000 cells.

To detect the expression of p53, the HepG2 cells were seeded at 1 × 106 cells / well on microscope cover glasses in 6 - well plates overnight before being treated with the drugs for the appropriate time periods, followed by washing with phosphate - buffered saline and fixing with ethanol.

Wing and leg discs were dissected from post-defecation prepupae under RNAse free conditions in 1X phosphate - buffered saline.

The tissues were dissected into small pieces, fixed with 4 % paraformaldehyde (PFA) in phosphate - buffered saline (PBS), and washed with PBS.

The lungs were washed twice, with 1 ml of phosphate - buffered saline, pH 7.4, and 2 ml of bronchoalveolar lavage (BAL) fluid was collected.

The spectroscopic data obtained of purified protein in phosphate - buffered saline (PBS) are summarized in Table 1.

For that purpose, 21 - to 23 - month - old mice were treated with daily injections of either recombinant GDF11 (rGDF11, 0.1 mg / kg mouse body weight), a dosing regimen that increases GDF11 levels in old mice toward youthful levels (13), or phosphate - buffered saline (PBS)(vehicle) for 4 weeks, and their blood vessels were subsequently analyzed by using the volumetric assay described above.