Sentences with phrase «pluripotent stem cell culture»

Laminin - 521 human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno - free and biologically relevant cell culture system.

Not exact matches

Finally, they demonstrated that zebrafish OPCs differentiate into mature oligodendrocytes when cultured together with human motor neurons, differentiated from induced pluripotent stem cells.

In practice, however, lung cells — especially from older, ill patients — won't grow well enough in culture, but will have to be produced from stem cells or induced pluripotent stem (iPS) cells, she says.

In the monkey, when we tried injecting 20 or 30 laboratory - cultured pluripotent stem cells, nothing happened.

All it took to make pluripotent stem cells, they reported, was briefly bathing blood cells from newborn mice in a mildly acidic solution and then tweaking the culture conditions.

A new study in Nature Genetics identifies a specific population of pluripotent embryonic stem cells that can reprogram to totipotent - like cells in culture.

Haruko Obokata of the RIKEN Center for Developmental Biology (CDB) in Kobe, Japan, and colleagues at other Japanese institutions and at Harvard Medical School in Boston reported that simply subjecting blood cells from newborn mice to a moderately acidic environment for 25 minutes and then tweaking culture conditions could generate pluripotent stem cells capable of developing into nearly all of a body's cell types.

«Studies on embryonic development greatly benefit from the culture system of embryonic stem cells and, more recently, induced pluripotent stem cells.

In culture dishes, ramping up the expression of just four genes can turn skin and other cells into so - called induced pluripotent stem (iPS) cells.

When BMP protein was added for three days in human pluripotent stem cell - derived gut tube cultures, it induced a posterior HOX code.

«Induction of Pluripotent Stem Cells From Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors,» by Kazutoshi Takahashi and Shinya Yamanaka, Cell, Aug. 25, 2006.

It facilitates self - renewal of both pluripotent human embryonic stem cells and induced pluripotent stem cells in a chemically defined, feeder - free and animal - protein free cell culture system.

We use neuronal cultures, induced pluripotent stem (iPS) cells and transgenic and gene - targeted mouse models to study the differential effects of apoE3 and apoE4 on cell signaling pathways and cytoskelatal structure and function at molecular, cellular and behavioral levels.

All the hES and human induced pluripotent stem cell lines were confirmed to be genetically stable and pluripotent in in vivo and in vitro assays after extensive culturing on the new substrata.

Culture of Pig Induced Pluripotent Stem Cells without Direct Feeder Contact in Serum Free Media.

The DEF - CS system is a robust culture system for efficient expansion of human induced pluripotent stem (iPS) cells in a feeder - free and defined environment.

Gage's team used human pluripotent stem cells to develop brain organoids, which were grown in culture for 40 to 50 days.

LN521 based stem cell cultures grow as monolayers on top of the laminin substrate and remain pluripotent without spontaneous differentiation.

StemFit Basic02 is a xeno - free, defined medium for human pluripotent stem cell (hiPSC) culture that offers an effective solution for regenerative medicine research.

Embryonic stem cells — the subject of so much controversy (witness the new ruling blocking their use)-- were first cultured in the lab a little more than a decade ago; in 2006, there was another breakthrough when adult cells were coaxed into becoming induced pluripotent stem cells.

One Standardized Differentiation Procedure Robustly Generates Homogenous Hepatocyte Cultures Displaying Metabolic Diversity from a Large Panel of Human Pluripotent Stem Cells.

The study used three cell types: human motor neurons derived from induced pluripotent stem cells, rat cortical cultures, and human breast cancer cells.

The portfolio includes U.S. and foreign patents and applications directed to culture, expansion and scale - up of pluripotent stem cells and cell types derived therefrom, various differentiation processes for production of mesendoderm, definitive endoderm, PEC - 01, and endocrine precursors, as well as claims directed to the Company's Encaptra ® macro-encapsulation device platform.

As bioinformatics approaches can only provide partial solutions, a functional analysis pipeline, based on primary (fibroblast) cell cultures, induced Pluripotent Stem cells (iPS) or simple model organisms, is truly essential.

The researchers cultured human induced pluripotent stem cells (iPSCs) to create three - dimensional mini-brains for study.

In this study, we analyzed three different cultures of neural cells carrying trisomy of chromosome 21, which were generated by directed differentiation from induced pluripotent stem cells (iPS cells).

In addition, pluripotent stem cells tend to be genetically unstable, particularly if less than optimal culture techniques are used.

For the past three years, McDevitt and his team have been working to culture viable human V2a interneurons from pluripotent stem cells.

Culturing pluripotent stem cells, including embryonic stem cells, is much more complicated and expensive than culturing most other ceCulturing pluripotent stem cells, including embryonic stem cells, is much more complicated and expensive than culturing most other ceculturing most other cell types.

When Po - Lin So contacted me about the position in Gladstone's Stem Cell core facility, I realized it was the perfect opportunity to use my experience with iPSC (induced pluripotent stem cell) culture and differentiation, as well as gain new skills and further my career groStem Cell core facility, I realized it was the perfect opportunity to use my experience with iPSC (induced pluripotent stem cell) culture and differentiation, as well as gain new skills and further my career groCell core facility, I realized it was the perfect opportunity to use my experience with iPSC (induced pluripotent stem cell) culture and differentiation, as well as gain new skills and further my career grostem cell) culture and differentiation, as well as gain new skills and further my career grocell) culture and differentiation, as well as gain new skills and further my career growth.

Last year, this team reported recurrent changes in the genomes of human pluripotent stem cells as they are expanded in culture.

Due to the large number of hES cells and human induced Pluripotent Stem (hiPS) cells needed by I - Stem's research groups, the development of large - scale culture systems for expansion and differentiation of those cells is critical.

Since we observed robust expression of SOX2 and modest expression of c - MYC and KLF4 in our human NSC cultures, we tested if ectopic expression of OCT3 / 4 per se would be sufficient to convert them from multipotent to pluripotent stem cells.

Here we report that cultures of expanded potential stem cells can be established from individual eight - cell blastomeres, and by direct conversion of mouse embryonic stem cells and induced pluripotent stem cells.

◊ detection of mycoplasms in the cultures of cellular lines: March 23 or October 18 in Illkirch - Strasbourg (CFE, PHENOMIN - ICS) ◊ technical assessment of pluripotent stem cells: customized training in Lyon (AniRA)

Cells from these embryos can be used to create pluripotent stem cell «lines» — cell cultures that can be grown indefinitely in the laboratory.

This poster describes the isolation and maintenance in culture of pluripotent stem cells, their differentiation, and the generation and potential uses of organoids.

Rhinos are much less studied than mice — but Jeanne Loring, director of the Center for Regenerative Medicine at the Scripps Research Institute, has already turned northern white rhino cell cultures into pluripotent stem cells.