Sentences with phrase «proavp surface marker»

His work indicates that this cell surface marker could serve as a target for a novel brain cancer vaccine or T - cell therapies engineered to recognize and kill tumors carrying that neoantigen.

Lipke, who specializes in the structure and function of cell surface markers in yeasts, says Rezende was as independent as a typical late - term Ph.D. student.

For instance, LKB1 also helped to repress cell surface markers on immune cells called dendritic cells that fueled the allergic reaction.

Several studies have used cell - surface markers — proteins found on the outer membranes of tumor cells — to identify glioblastoma stem cells; but the specific markers used have been controversial and can not reflect molecular processes going on within tumor cells.

T - lymphocyte cells are distinguished by surface markers.

To prove this point, he and Saito applied their miRNA switches to the purification of hepatocytes and pancreatic cells, neither of which has unique cell surface markers, finding miRNA switches effective there too.

The use of cell surface markers to isolate specific cell populations is one common method for separating cells; however, isolating live cells based on their RNA expression is a powerful new way enabling the study of small cell niches in nongenetically modified animal models and human tissue.

Without it, NFAT initiates a negative regulatory program that activates genes encoding inhibitory cell surfaces markers and blunts signals received by the T cell receptor.

At this time, it is only possible to verify the decision retrospectively with cell surface markers,» explains Dr. Carsten Marr, head of the Quantitative Single Cell Dynamics Research Group at the Helmholtz Zentrum München's Institute of Computational Biology (ICB).

Researchers at Karolinska Institutet have identified cell surface markers specific for the very earliest stem cells in the human embryo.

«We've not had cell surface markers for the different stem cell states before, which has made it hard to study them,» says Fredrik Lanner, assistant professor at Karolinska Institutet's Department of Clinical Science, Intervention and Technology.

«Only a subset produces IL - 17A upon skin injury, although the surface markers on these cells seem identical to those of other DETCs,» MacLeod said.

Cancer cells settle to the bottom of the well, where they selectively bind to the antibodies based on their surface markers (unlike other devices, the chip can also trap tiny structures called exosomes produced by cancers cells).

Once they were able to isolate skeletal muscle cells using the newly identified surface markers, the research team matured those cells in the lab to create dystrophin - producing muscle fibers.

Using the ERBB3 and NGFR surface markers, the skeletal muscle cells were isolated and then injected into mice at the same time a TGF Beta inhibitor was administered.

Skeletal muscle cells isolated using the ERBB3 and NGFR surface markers (right) restore human dystrophin (green) after transplantation significantly greater than previous methods (left).

Significantly, cells with reduced mtDNA became self - renewing and expressed specific cell surface markers characteristic of breast cancer stem cells.

Wang explained that, because ECMs are composed of collagen, elastin, carbohydrates and signaling molecules and have no cell surface markers, DNA or RNA from the donor, the recipient is less likely to reject the treatment.

The mouse monoclonal antibody they have developed, MAG - 1, targets the ProAVP surface marker.

In breast cancer, CSCs or tumor - initiating cells were first identified by using a combination of cell surface markers, CD24 − / CD44 + / ESA (EpCAM) + (2).

These results suggest an existence of wide range of heterogeneous population of CSCs although they still share the self - renewal ability and same surface markers.

We will develop mathematical models to predict the pathways of differentiation from naive to memory and effector T - cell subsets based on the characterisation of surface marker expression, transcription factors and cytokines production at early and late time points after immunisation.

«But the desired cells often lack a readily accessible surface marker, or that marker is not specific enough, as is the case for cardiac muscle cells.

The scientists also found that a protein known as CD29 acts as a cell - surface marker for precursor fat cells that can generate mature cells with high energy potential, as shown by their UCP1 expression.

But the new data reveals that oRGs bring a support group with them: The cells express genes for surface markers and molecular signals that enhance their own ability to proliferate, the researchers found.

Phenotypic analyses of cell surface markers in spleen, lymph node, and thymus were performed on unchallenged mice at 6 — 8 wk of age.

MSC control surface marker expression to exhibit a hypoimmunogenic or tolerogenic phenotype.

Asialoglycoprotein receptor 1 is a specific cell - surface marker for isolating hepatocytes derived from human pluripotent stem cells.

To compare T cell phenotypes between DGKζ − / − and Cbl - b − / − mice, and to evaluate DKO mice, we isolated thymi, lymph nodes, and spleens from mice of each genotype and determined expression of T cell surface markers.

Differential expression of surface markers in mouse bone marrow mesenchymal stromal cell subpopulations with distinct lineage commitment.

The authors used the cell surface marker podoplanin as a handle for isolating the progenitor cells from bone marrow.

Another liver - specific protein, Asialoglycoprotein receptor 1 (ASGPR1), is a surface marker for mature hepatocytes [5, 6].

Macrophages were identified by their typical morphology, as well as by their reactivity to the cell surface marker F4 / 80 (Fig. 2A) ⇓.

[B and E] Brain leukocytes were percoll purified from the remaining brain and meningeal homogenate, stained for leukocyte surface markers CD4, CD8, CD19, CD11b, and Ly6G, and assayed using flow cytometry.

Lineage - restricted progenitors are identifiable by expression of distinct combinations of cell surface markers.

Interestingly, an increased fraction of the cell population was found to express CD44 + / CD24 - / ESA + cell surface markers.

Since very few studies have demonstrated the formation of macro-metastasis from low numbers of sorted CSCs and currently proposed CSC markers might not be sufficient to identify all stem cell populations [13], we believe that our study presents a promising approach to isolate stem - like and metastatic breast CSCs as opposed to the cell - sorting strategy based on putative stem cell surface markers.

Every T cell type expresses different cell surface markers and secretes different cytokines.

[B] Brain leukocytes were percoll purified from the remaining brain and meningeal homogenate, stained for surface markers CD4, CD8, CD19, CD11b, and Ly6G, and assayed using flow cytometry.

To characterize activation of immune cells, measurement of intracellular signaling using phospho - specific antibodies combined with immune phenotyping using surface markers is ideal.

Cells were stained with antibodies specific for human cell surface markers: CD326 / ESA - FITC, CD24 - PE, and CD44 - PE - Cy7 (BD Pharmingen, San Jose, CA, USA).

Luckily, different cell types tend to have different things on their surfaces, which play particular parts in their specialized roles in the tissue, so it is a matter of identifying and targeting cell - surface markers that are specific to these abnormal cell types.

By means of a non-obese diabetic / severe combined immunodeficiency disease (NOD / SCID) xenotransplant assay in combination with specific cell surface markers (CD44 + CD24 - / low), CSCs were enriched from metastatic and primary breast tumors and were shown to have the ability to reestablish tumor heterogeneity after transplantation [1].

Positive clones were then differentiated using defined serum - free conditions outlined in Ng, et al [7], which overall gave rise to a CD45 + CD56 + CD117 − CD94 + population of cytotoxic NK cells [8] expressing the CD4 construct and other surface markers in a similar manner to peripheral blood NK cells.

Based on cell surface markers known as CD4 and CD8, T cells generally fall into two broad categories: CD4 — positive helper T cells, which ¬ help activate other immune cells and CD8 - positive cytotoxic T cells, which kill cells that are cancerous or infected with viruses.

Cells were washed with 1 % BSA and stained with surface markers for ILC3.

g defined serum - free conditions outlined in Ng, et al [7], which overall gave rise to a CD45 + CD56 + CD117 − CD94 + population of cytotoxic NK cells [8] expressing the CD4 construct and other surface markers in a similar manner to peripheral blood NK cells.

Here we consider two surface markers thought to define these cells in mice, CD8 and Killer cell lectin - like receptor G1 (KLRG1), and a means we developed to remove these cells from the blood of aged C57BL / 6 mice.

However, SSEA1 can be a useful cell surface marker for the detection and enrichment of putative germ cells derived from ES cells as reported previously [56].

(A) Flow cytometry of two independent HV clones (HV 5.1 and HV 16.1) cultured either under self - renewing conditions or in the absence of LIF show the presence of a subpopulation of cells positive for Venus and / or the ES cell surface marker SSEA - 1.