Sentences with phrase «pymt +»
c. For generation of the MMTV - PyMT + / tg; Postn + / + control female mice, the MMTV - PyMT + / tg; Postn + / − male mouse will be crossed to Postn + / + female mice to obtain MMTV - PyMT + / tg; Postn + / + male mice that will then be crossed with Postn + / + female mice.
https://elifesciences.org/
MMTV - PyMT + / tg; Postn − / − mice and their Postn + / + counterparts were analyzed for primary tumor formation and metastasis.
Monitor MMTV - PyMT + / tg; Postn + / + and MMTV - PyMT + / tg; Postn − / − female mice for tumor development and keep until tumor disease is fully developed and the metastatic disease is estimated to occur.
◯ Number of pulmonary macrometastases in MMTV - PyMT + / tg; Postn + / + mice relative to MMTV - PyMT + / tg; Postn − / − mice.
Immediately after counting macrometastasis, use the first six lungs identified from MMTV - PyMT + / tg; Postn + / + female mice that are positive with metastatic disease for further analysis (Protocol 2).
b. For generation of the MMTV - PyMT + / tg; Postn − / − experimental female mice, male MMTV - PyMT + / tg; Postn − / − male mice will be crossed with Postn − / − female mice.
Breed MMTV - PyMT + / tg; Postn + / − or MMTV - PyMT + / tg; Postn − / − male mice with Postn + / + and Postn − / − female mice to obtain MMTV - PyMT + / tg; Postn + / + control and MMTV - PyMT + / tg; Postn − / − experimental female mice, respectively.
Not exact matches
Female mice carrying the MMTV - PyMT transgene that are either Postn + / + or Postn − / − will be examined for changes in primary tumor size and the number of spontaneously formed pulmonary macrometastases, which is a replication of the experiment reported in Figures 3A, 3B, and Supplemental Figure 13.
Using the MMTV - PyMT mouse breast cancer model, which spontaneously metastasizes to the lungs, Malanchi and colleagues reported that only the CSC population, identified as CD24 + CD90 +, were capable of initiating lung metastases and secondary metastases (Guy et al., 1992; Lin et al., 2003; Malanchi et al., 2012).
We also performed microarray analysis of PyMT - Slc6a14 + / + and PyMT - Slc6a14 − / − mammary tumours to evaluate the expression of amino acid transporters without any biased preconception of the identity of the transporters that might be subject to changes in expression in association with Slc6a14 deletion.
(B) Representative images of the excised tumours and lung metastatic nodules (black arrows) from 4 - month - old Slc6a14 + / + / PyMT mice and Slc6a14 − / − / PyMT mice.
With 12 different amino acid transporters examined by RT - PCR, none of the transporters differed in expression between PyMT - driven tumours with Slc6a14 + / + and Slc6a14 − / − backgrounds (result not shown).
In 4 - month - old PyMT - Slc6a14 + / + mice, metastatic nodules were detectable in the lung but there was no metastasis in age - matched PyMT - Slc6a14 − / − mice (Figure 2B).
At 3 months of age, all Slc6a14 + / + females with PyMT transgene developed tumours in multiple mammary glands which grew to sizes of 2000 — 2500 mm3 at 4 months of age (Figure 2B).