Sentences with phrase «pymt female»
a. Do not use MMTV - PyMT female mice for breeding as they develop mammary tumors.
https://elifesciences.org/ Not exact matches
Breed MMTV - PyMT + / tg; Postn + / − or MMTV - PyMT + / tg; Postn − / − male mice with Postn + / + and Postn − / − female mice to obtain MMTV - PyMT + / tg; Postn + / + control and MMTV - PyMT + / tg; Postn − / − experimental female mice, respectively.
b. For generation of the MMTV - PyMT + / tg; Postn − / − experimental female mice, male MMTV - PyMT + / tg; Postn − / − male mice will be crossed with Postn − / − female mice.
Immediately after counting macrometastasis, use the first six lungs identified from MMTV - PyMT + / tg; Postn + / + female mice that are positive with metastatic disease for further analysis (Protocol 2).
Female mice carrying the MMTV - PyMT transgene that are either Postn + / + or Postn − / − will be examined for changes in primary tumor size and the number of spontaneously formed pulmonary macrometastases, which is a replication of the experiment reported in Figures 3A, 3B, and Supplemental Figure 13.
Monitor MMTV - PyMT + / tg; Postn + / + and MMTV - PyMT + / tg; Postn − / − female mice for tumor development and keep until tumor disease is fully developed and the metastatic disease is estimated to occur.
c. For generation of the MMTV - PyMT + / tg; Postn + / + control female mice, the MMTV - PyMT + / tg; Postn + / − male mouse will be crossed to Postn + / + female mice to obtain MMTV - PyMT + / tg; Postn + / + male mice that will then be crossed with Postn + / + female mice.
PyMT - Tg female mice typically develop tumours at the age of ~ 10 weeks.
We examined the expression of Slc6a14 in breast tumours obtained from 4 - month - old PyMT - Tg female mice and in mammary glands obtained from age - matched wild - type female mice.
At 3 months of age, all Slc6a14 + / + females with PyMT transgene developed tumours in multiple mammary glands which grew to sizes of 2000 — 2500 mm3 at 4 months of age (Figure 2B).
In contrast, no Slc6a14 − / − female with PyMT transgene developed tumours at 3 months of age; however, at 4 months of age, small tumour nodules became detectable (Figure 2B), indicating a marked delay in mammary tumour development in PyMT - Tg mice as a consequence of Slc6a14 deletion.