Sentences with phrase «pyrosequencing using»

Pyrosequencing using the Titanium method was carried out using the Titanium genomic kit.

Not exact matches

Pbo's group used a technique developed by Branford, Conn. - based biotech company 454 Life Sciences called pyrosequencing, which takes advantage of the fact that ancient DNA is often fragmented.

Microbial DNA from colon specimens were isolated and analyzed by use of 454 pyrosequencing of 16S RNA genes.

The team employed a new, massively parallel technique called pyrosequencing, which uses pulses of light to read the sequence of thousands of bases at once.

DNA samples are prepared from microbial specimens, and then analyzed using massively parallel sequencing methods such as 454 / Roche pyrosequencing [20].

Because < 20 % of all gastrointestinal bacteria can be cultured using conventional methodologies, we performed massively parallel pyrosequencing to further assess the qualitative changes in the intestinal microbiome following alcohol exposure.

Using pyrosequencing to determine mtDNA carryover they found that using frozen rather than fresh eggs leads to higher carryUsing pyrosequencing to determine mtDNA carryover they found that using frozen rather than fresh eggs leads to higher carryusing frozen rather than fresh eggs leads to higher carryover.

Here we use pyrosequencing of the bacterial 16S rRNA gene to quantify bacterial taxa [21].

Purified amplicon DNAs were quantified using Quant - iT PicoGreen kit (cat # P7589, Invitrogen, Carlsbad, CA) and pooled for pyrosequencing.

Mostafa Ronaghi, Ph.D., Stanford Genome Technology Center, Palo Alto, Calif. $ 1.8 million (3 years) «Pyrosequencing Array for DNA Sequencing» The principal investigator of this team is an inventor of pyrosequencing, which uses unmodified nucleotides and polymerases to synthesize DNA and a firefly enzyme to generate a chemiluminPyrosequencing Array for DNA Sequencing» The principal investigator of this team is an inventor of pyrosequencing, which uses unmodified nucleotides and polymerases to synthesize DNA and a firefly enzyme to generate a chemiluminpyrosequencing, which uses unmodified nucleotides and polymerases to synthesize DNA and a firefly enzyme to generate a chemiluminescent signal.

Services include: consultation with investigators to chose the best method of analysis; sample preparation, assay design and processing of the most used methods to study DNA methylation, both gene - specific and genome - wide (PyroMeth, RRBS and Methyl - Seq); and assessment of global DNA methylation by pyrosequencing methylation analysis of repetitive elements (LINE - 1 and Alu repeats).

The researchers then analyzed the types of bacteria present in each sample using a technique called 454 pyrosequencing.

Molecular assessment of the fecal microbiota in healthy cats and dogs before and during supplementation with fructo - oligosaccharides (FOS) and inulin using high - throughput 454 - pyrosequencing

We have further confirmed methylation differences at several sites using two different techniques, Q - MeDIP (Figures S2A and S2B) and pyrosequencing (Figure S2C) as described in the Methods section.