Sentences with phrase «pyruvate supplements»

Pyruvate supplements are available in capsule, tablet, and bulk powder form.

Overall, the difference in weight loss between the pyruvate supplement and placebo groups was not large, amounting to approximately 1.1 - 1.6 kg (about 2 - 3 lb) additional weight loss in the pyruvate groups.

Not exact matches

On arrival, islets were immediately placed in CMRL 1066 (Cellgro; Corning) enriched medium, supplemented with 2 mM l - glutamine (Cellgro CN - 25 -005-CI; Corning), 1 mM sodium pyruvate, (Cellgro CN -25-000-CI; Corning), 10 mM Hepes - free acid, (Cellgro CN - 25 -060-CI; Corning), 10 U / ml heparin (Hospira RL - 4311; Pfizer), and 10 mM nicotinamide, (CN N0636; Sigma - Aldrich) at 24 °C with 5 % CO2 for 7 d to stabilize the islets before starting the experiments.

The supernatants were discarded, and the tissue was resuspended in DMEM supplemented with 10 % fetal bovine serum, 2 - mm l - glutamine, sodium pyruvate, nonessential amino acids, and a vitamin solution (Life Technologies, Inc., Rockville, MD).

CMEM consisted of MEM supplemented with 10 % FBS, 2 mM l - glutamine (BioWhittaker), 1 mM sodium pyruvate (BioWhittaker), 2 mM MEM vitamins (Bio Whittaker), and 1 % penicillin - streptomycin - fungizone solution (BioWhittaker).

MDA - MB 231 and BCM2 cell lines were cultured in minimum essential medium (MEM)(Invitrogen Corporation, Carlsbad, CA, USA) supplemented with 10 % fetal bovine serum (HyClone, Logan, UT, USA; Thermo Fisher Scientific Inc., Waltham, MA, USA), 2 mM L - glutamine, 1 mM sodium pyruvate, 1 mM non-essential amino acids, and 1 % vitamin (HyClone).

Goat fetal fibroblasts were isolated as described previously [22] and cultured in DMEM supplemented with 15 % FBS, 1 % sodium pyruvate and 1 % penicillin streptomycin to achieve 80 — 90 % confluency on the day of transfection.

ACHN cells (ATCC, CRL - 1611) were maintained in EMEM supplemented with 10 % FBS, 2 mM glutamine, and 1 mM sodium pyruvate.