Sentences with phrase «rcs rats»

Of note, while vision rescue requires preservation of some functional photoreceptors, the mere presence of photoreceptor cells in the ONL of transplanted RCS rats does not assure function [61].

Further sham - operated RCS rats from separate, concurrent studies [42], [43] were available for comparison and yielded similar results (these animals were not included in the present study).

Histological examination reveals that hNPCctx survive, migrate and assume two substantially different appearances and distributions following transplantation into the subretinal space of RCS rats.

ERG response amplitudes to full field light stimulation were recorded at approximately P100 in RCS rats injected with hNPCctx — GDNF (n = 9), hNPCctx (n = 21) or medium alone (n = 3) into the subretinal space.

In dystrophic RCS rats, threshold levels at P100 are greater than 3.0 log units above the background level of 0.02 log candela / m2.

It has also been shown that fibroblasts [43], [60] and placenta - derived progenitor cells [43] are unable to maintain visual functions long - term, further indicating that the rescue effects seen in the RCS rat model are not the result of a non-specific event.

Using three independent tests performed at P90 - 100, hNPCctx - and hNPCctx - GDNF - transplanted eyes demonstrated retention of visual functions at levels among the best documented in the RCS rat model [29], [38], [40]--[43], [56], [59], [60].

In the scotopic - adapted RCS rat, the ERG a-wave (indicative mainly of rod activity) disappears by P60, while the composite b - wave (comprising rod and cone activity) is largely lost around P100 [39].

This report is significant for the unique disposition of the transplanted hNPCctx and for the subsequent levels of functional rescue achieved, which are among the best encountered in the RCS rat [29], [38]--[43].

Of note, the sham responses obtained in these experiments were essentially identical to those obtained in other studies using the RCS rat [43].

iPS - RPE cells were injected into the subretinal space between the host RPE and photoreceptor cells (B) A layer of iPS - RPE cells in the subretinal space of the dystrophic RCS rat 20 hours following transplantation.

The progression of retinal dystrophy in the RCS rat is such that by 13 weeks post-graft most of the ONL has disappeared [33] and the photoreceptor outer segment layer is reduced to a debris zone [40], a finding we observed in dystrophic controls.

iPS - RPE cells were transplanted into dystrophic RCS rat eyes at three weeks of age, a time when retinal abnormalities are first observed.

(D) Expression of the same RPE cell markers is maintained in vivo by iPS - RPE (white arrows) 8 days following transplantation into the subretinal space of the RCS rat.

(A) Extensive preservation of the nuclear photoreceptor layers in the dorsal retina of the dystrophic RCS rat 13 weeks following transplantation of iPS - RPE cells (DAPI stained nuclei).

We used the head - tracking response to moving vertical lines to examine visual acuity in the RCS rat 13 weeks following surgery.

A head - tracking response is observed in the dystrophic RCS rat during optokinetic testing of the left eye, 13 weeks after iPS - RPE cell transplantation.

Previous studies, using electroretinography to assess function following cell transplantation into the RCS rat have only been able to demonstrate global activity across the retina [6], [41]--[43].

Importantly, akin to HESC - RPE in vivo, cells at the outside edge of the iPS - RPE cell bolus could phagocytose photoreceptor outer segments from the RCS rat, as indicated by the presence of rhodopsin - positive photoreceptor material within the cellular membrane of iPS - RPE labelled with HSM (Fig. 5E).

Inset shows higher resolution confocal images of photoreceptor cell nuclear layers (DAPI blue) and rhodopsin expression (red) in the dystrophic control (left inset) and dystrophic with iPS - RPE transplant (right inset) RCS rat.

The major limitation of this study is the rejection of human iPS - RPE after transplantation into the RCS rat.

No head - tracking response is observed in the 16 - week old dystrophic RCS rat during optokinetic testing of the right, non-transplanted, eye.

Not exact matches

We transplanted unmodified and GDNF - expressing hNPCctx (hNPCctx - GDNF) to the subretinal space of the Royal College of Surgeons (RCS) rat.

We used the head - tracking response to assess the visual function of RCS dystrophic rats 13 weeks after receiving a subretinal iPS - RPE transplantation in one eye only (Fig. 6 and Movies S2 and S3).

In order to test the therapeutic efficacy of iPS - RPE, we transplanted cells into the subretinal space of RCS dystrophic rats (Fig. 5A).

(A) Preservation of optokinetic head - tracking response to a rotating vertical stimulus in 16 - week - old RCS dystrophic rats following transplantation of iPS - RPE.

iPS - RPE are capable of phagocytosing photoreceptor material, in vitro and in vivo following transplantation into the Royal College of Surgeons (RCS) dystrophic rat.

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