Sentences with phrase «rna binding»

However, we have shown that a loss - of - function mutation in the gene encoding TAR RNA binding protein 2 (TARBP2) leads to delayed activation of Prm1 translation and sterility.

We are investigating the roles of long non-coding RNAs, piRNAs, and of non-canonical RNA binding proteins in Drosophila development.

Writing in Science, the team found two RNA binding proteins, ZFP36L1 and ZFP36L2, are key in dictating the timings of these stages.

For example, our studies identified transcription factors, RNA binding proteins, and signaling genes as critical miRNA targets during contexts such as development of peripheral sensory organs, wing, eye, and CNS, or during behavioral contexts such as egg - laying, rhythmic behavior, and locomotor activity (Figure 2).

Neural specificity of the RNA binding protein Elav is achieved by post-transcriptional repression in non-neural tissues.

This RNA binding protein, upregulated upon B cell activation, post-transcriptionally regulates components of the DNA damage repair machinery, such as p53, silencing the mRNA and keeping the transcripts captive in RNA stress granules.

Furthermore, the RNA binding domains of these proteins do not contain any introns, whereas Boule genes contain a conserved genomic structure with at least two introns separating the RRM - encoding exons at conserved junctions.

This is consistent with the higher sequence divergence of the C. elegans daz - 1 RNA binding motif than most other bilaterian Boule homologs (Figure 2).

We replaced exon 3, which encodes a part of the RNA binding domain and is present in all Boule isoforms (Figure 3C), with a lacZ - neo vector through homologous recombination in embryonic stem cells.

The removal of exon 3 resulted in a deletion of the RNA binding domain and a frame - shift in the remaining transcript.

This transcript is expected to produce a truncated BOULE protein missing both its RNA binding domain and the remaining C - terminal portion of the protein.

(A) Alignment of Boule RRM domains from representative species of both deuterostomes and protostomes reveals conservation of the RNA binding domain, in particular in the regions surrounding RNP2 and RNP1 as well as the C - terminal region.

Alignments for RNA binding domains (RRM) from insects (A) and mammals (B) reveal strong conservation among homologs.

For nearly a decade, Sattler made contributions to RNA research, working to tease out how RNA binding proteins uniquely bind to specific RNA and regulate gene expression.

Now, in his dual - role as professor of Biomolecular NMR - Spectroscopy at the Technical University Munich (TUM) and Director of the Institute of Structural Biology at Helmholtz Zentrum München, Sattler has several projects underway — many investigating protein - RNA binding, some using structural biology to guide drug discovery — but one consistent theme is his use of integrated methods.

The Cheng lab develops technologies based on artificial DNA and RNA binding proteins and applies them to sense or induce sequence or epigenetic changes at target genomic loci or target transcripts.

In 2011, Michael Sattler took a look at an RNA binding protein that was known, based on earlier X-ray crystallography work, to have a structure with a specific arrangement of two RNA binding domains bound to its RNA ligand.

«I started to realize there are all these interesting RNA binding proteins that are not well understood,» says Sattler, who also expanded his solution - based methods to include small angle x-ray scattering and small angle neutron scattering while at EMBL.

Functional analysis of genes with recurrent exons (i.e., with association with PMI in more than two tissues) shows a noteworthy enrichment on RNA binding and RNA splicing genes (Supplementary Fig. 18b, c).

In 2012 she joined the laboratory of Dr. D.L. Konotyiannis at BSRC AL.Fleming, Greece as postdoctoral scientist, where she worked on the study of the RNA Binding Protein HuR during intestinal and lung inflammation and cancer, using inducible, KO and Tg mouse models and xenografts.

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by motor neuron death and muscle atrophy, and the RNA binding protein TDP - 43 has emerged as a major molecular factor in this disease.

By mutating residues in ADAR2 that interact with duplex RNA, they hoped to destabilize ADAR - RNA binding to decrease off - targets.

Specific RNA binding to ordered phospholipid bilayers.

Using mouse skeletal muscle stem cells as a model, we show that accumulating transcripts specifying the myogenic program are not translated in quiescent satellite cells, but are repressed by the action of microRNAs and RNA binding proteins.

A conserved role for the zinc finger polyadenosine RNA binding protein, ZC3H14, in control of poly (A) tail length.

This additional mutation is located in an area of the structure where it binds to RNA, known as the RNA binding groove.

hnRNP A1 is a multifunctional RNA binding protein which is involved in pre-mRNA splicing, export of mature transcripts from the nucleus, mRNA turnover, and internal ribosome entry site (IRES)- mediated translation.

Hfq binds 17S rRNA using its distal surface, which is distinct from the surface governing the small RNA binding.

Indeed, we have shown that Sec16, a protein related to ER exit sites and protein transport, is also required in stress granule formation specifically during amino - acid starvation because it binds a modified form of the RNA binding protein Rasputin and stabilises it (9).

An international team of scientists, including four from Brown University, conducted and analyzed tests using a «knock - in» mouse carrying a gene for a mutant DNA / RNA binding protein called TDP - 43, which causes a form of inherited ALS in humans.

Antibodies to the RNA Binding Protein Heterogeneous Nuclear Ribonucleoprotein A1 Colocalize to Stress Granules Resulting in Altered RNA and Protein Levels in a Model of Neurodegeneration in Multiple Sclerosis.

RNA biology, genomics, RNA binding proteins, miRNAs, alternative splicing, translation, brain tumors

According to the researchers, Hnrnph1 codes for an RNA binding protein that regulates the processing of hundreds of other genes in the brain.

We use a combination of genomics, systems biology, biochemistry, bioinformatics and molecular biology to investigate the networks formed by RNA binding proteins, miRNAs and their target genes and evaluate their impact on biological processes, cancer and disease states.

The discovery has implications for other, more common forms of ALS that have accumulations of other over-gelled RNA binding proteins.

They suspected that MSI1 — an RNA binding protein — might be important in this process because it is involved in regulating the pool of neural stem cells that are required for normal brain development.

A study of gene expression in leukemia cells has identified an RNA binding protein that plays an important role in driving the development of cancer.

In the case of IGF2BP3 and B - cell leukemia, the overall effect of the RNA binding protein is to promote the proliferation of B cells by shifting the expression of a large number of genes, Sanford said.

Many factors are involved in controlling which genes get transcribed into messenger RNA and when, but RNA binding proteins interact with the messenger RNA itself to regulate gene expression after transcription has occurred.

In testing on laboratory mouse models and human MDS / AML samples, the researchers identified a novel substrate of TRAF6 called hnRNPA1, an RNA binding protein.

«Role of RNA binding protein in driving cancer, leukemia study reveals: Abnormally expressed in cancer cells, the protein was found to promote the proliferation of B cells in B - cell acute lymphoblastic leukemia.»

Compared to other proteins involved in regulating gene activity, RNA binding proteins have not been well studied.

The RNA binding domain (TRBD) of telomerase is a crucial component to this process and, therefore, the enzyme's ability to work.

«So, a variant that affects the target sequence could reduce guide RNA binding.

This «antisense» RNA binds to RNA molecules from the faulty region; as a result, the splicers interpret the mutation as junk and eliminate it.

Dengue subgenomic RNA binds TRIM25 to inhibit interferon expression for epidemiological fitness

When viral RNA binds TLR3, it kicks off an immune response.

This results in the messenger RNAs binding firmly to the protein.

In testing one of the fatty acids representative of those found before life began — decanoic acid — the scientists discovered that the four bases in RNA bound more readily to the decanoic acid than did the other seven bases tested.

In a recent Nature paper, Chi et al used HITS - CLIP to isolate RNA bound by the Argonaute protein (Ago), which mediates miRNA - mRNA interaction (see figure).