Sentences with phrase «rna editing site»
Using the technology, Pal's lab has made many seminal contributions, including evidence for the existence of the nuclear - encoded plastid transcription system and identification of key elements involved in promoter recognition, translational initiation, and RNA editing site specificity.
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Editing genes with CRISPR requires at least two components: a protein that cuts DNA and a piece of RNA that guides it to the precise DNA site to make the cut.
Using the model organism, Caenorhabditis elegans, the researchers identified over 400 new mRNA editing sites — the majority regulated by ADR - 1 — and declared the protein the first global regulator of RNA editing.
Our study reveals a family of endonucleases that use dual - RNAs for site - specific DNA cleavage and highlights the potential to exploit the system for RNA - programmable genome editing.
Each contained between 80,000 and 130,000 RNA sites that had been edited.
Regions surrounding chloroplast editing sites could be mutated in vitro and RNA transcribed from mutated templates.
Wagoner, J.A., Sun, T., Lin, L., Hanson, M.R. (2015) Cytidine deaminase motifs within the DYW domain of two pentatricopeptide repeat - containing proteins are required for site - specific chloroplast RNA editing.
For editing the genome, this system makes use of 3 components, a guide RNA (gRNA) of about 125 nt that specifies the target, the Cas9 endonuclease that creates the DNA double - strand break (DSB) at the target site, and a donor oligonucleotide or plasmid as the repair material if needed (for knock in models).
They successfully edited 33/34 sites with a maximum of 28 % editing efficiency in HEK293T cells, as assessed using RNA - seq.
«By combining different methods for RNA sequencing with new bioinformatics tools we could find novel sites of nucleotide base exchange, so - called editing», says Jens Lagergren, Professor at the Royal Institute of Technology and group leader at SciLifeLab Stockholm.
Cas9 is the endonuclease enzyme part of CRISPR / Cas9 system that cuts the DNA, while RNA is the CRISPR guide, directing the enzyme to specific sites in the genome so that precise genome edits are possible.