Sentences with phrase «rna extracts»

In order to determine what fraction of the total dsRNA was bound to RDE - 4 and to examine the requirements for the stability of the dsRNA bound to RDE - 4, RNA extracts from wild - type and rde mutant strains were analyzed.

To investigate whether the discovery of XMRV may have resulted from inadvertent laboratory contamination, we re-analyzed available archival RNA extracts from prostate cancer samples taken from the original 2006 study by Urisman, et al [1].

To prove the hypothesis that an XMRV - infected cell line had contaminated the prostate cancer samples in the 2006 Urisman, et al. study, we analyzed available RNA extracts using a novel technique referred to as mitochondrial RNA (mtRNA) profiling.

Deep sequencing of RNA extracts from the re-extracted archival VP62 (2012) tissue was also performed.

An RT - PCR assay for the detection of mouse IAP sequences was also performed on total RNA extracts from VP35 and VP42 and polyA RNA extracts from VP62 (2006)(as no total RNA was available) using previously published primers and conditions [42].

After contamination of the VP35 and / or VP42 sample (s) by XMRV - infected LNCaP, polyA RNA extracts from other prostate cancer samples then became cross-contaminated.

To characterize their viral genomes in greater depth, we analyzed RNA extracts from these 3 samples by unbiased next - generation, or «deep» sequencing.

Third - instar larvae were raised under the indicated nutritional conditions, and CCHa2 mRNA levels were tested by RT - qPCR using whole - animal RNA extracts.

(A) Relative amounts of dilp5 mRNA were quantified by RT - qPCR using whole animal RNA extracts from larvae at the late third - instar to wandering stages.

(B) dilp5 mRNA levels in mid-third-instar larvae (96 hours AEL) were quantified by RT - qPCR using whole larval RNA extracts.

Though initially substantiated with RNA extracted from shocked worms and injected into unconditioned ones, the memory transfer findings were questioned by a study the next year.

On the contrary, quantitative RT - PCR using the RNAs extracted from the MBs revealed that the difference in the Acks expression level in the MBs between the antennae - deprived and intact workers under high temperature was not statistically significant.

Quantitative RT - PCR using RNAs extracted from brain, thorax and abdomen of workers revealed that statistically significant and prominent Acks induction was observed only in the brain when the bees were exposed to high temperature, and scarce Acks expression was detected in the thorax and abdomen under both room and high temperatures (Figure 5O), further supporting that Acks induction under a high temperature reflects neural activity and does not result from some «heat shock responses» that could occur independently of neural activity.

Total RNA extracted from hand - dissected S. viridis inflorescence primordia at 15 DAS was used to synthesize cDNA for amplification of the bsl1 - 2 transcript products, which were purified, cloned, and sequenced (Figure 3C).

To ensure the detection of brain - specific changes in dilp5 expression, RNA extracted from the brain was used for RT - qPCR.

(A) RT - qPCR was performed on RNA extracted from larval tissues.

For this analysis, total RNA extracted from whole larvae was used, as dilp2 and dilp5 are predominantly expressed in the CNS during the larval stages examined [26,27].

Total RNA extracted from prostate cancer tissue cores was tested in the single - round XMRV pol RT - PCR assay utilizing the m2000rt system (Abbott Molecular, Inc.; Desplaines, IL).

Importantly, while the RNA from sample VP62 extracted in 2006 was positive for XMRV, RNA extracted from the same prostate tissue in 2012 was found to be negative not only by ViroChip and PCR (Fig. 2; Table 1) but also by deep sequencing, with no XMRV sequences detected out of 4 million deep sequencing reads (Fig. 5).

Not exact matches

Gene expression is the process by which cells extract information from genes and render it in the form of either protein or RNA molecules.

They extracted and sequenced RNA from 41 strains of HIV as well as 61 strains of hepatitis C virus infecting the children.

The study involved extracting Ribonucleic acid or RNA — found in the cells of all living organisms — to develop a transcriptome — the gene readouts in a cell — to examine what occurs during the different developmental stages of the cockroach pregnancy and to explore if those changes hold wider applications for other mammals.

The cells from which molecules were extracted remain alive, so researchers are free to sample the same live cell several times in order to analyse its RNA and proteins — and possibly even metabolites in the future.

Martins found the answer by extracting two molecules from the meteorite: uracil, a nucleobase found in RNA, and xanthine, an intermediate in the synthesis of DNA and RNA.

First, samples of leaves from these plants are collected for in vitro cultures to isolate the fungi; then the DNA and RNA of fungi are extracted to sequence them and, through bioinformatic analysis, the researcher can determine the expression, the presence or absence of genes in the genomes of a species against each other.

The synthetic poliovirus cDNA was transcribed by RNA polymerase into viral RNA, which translated and replicated in a cell - free extract, resulting in the de novo synthesis of infectious poliovirus.

Sogin began collecting and sifting through marine organisms — algae, fungi, sponges, jellyfish, anemones, mollusks — cutting them up and extracting DNA, adding enzymes, concentrating the DNA and sequencing the genes, reducing them to strips of code, comparing their ribosomal RNA, and applying algorithms to measure their relationship with one another and with insects, worms, fish, birds, and mammals.

Clariom Pico assays can extract data from as little as 100 pg of total RNA, from common and challenging sample types (including formalin - fixed, paraffin - embedded tissues and whole blood) without the need for globin messenger RNA reduction or ribosomal RNA removal.

Following isolation, RNA was extracted from each population, and the expression of three proinflammatory genes (Tnfa, Nos2, and Il6) was determined by quantitative RT - PCR.

Total RNA was extracted from frozen adipose tissue (100 mg), FACS - isolated cells (> 105), or cultured cells (60 - mm confluent plate) using a commercially available acid - phenol reagent (TRIzol; Invitrogen Corp.).

Total RNA was extracted from the perigonadal (epididymal or parametrial) adipose tissue of individual mice using a commercially available acid - phenol reagent (TRIzol; Invitrogen Corp., Carlsbad, California, USA).

RNA was extracted from geniculate ganglia of 5 - HT3AKO and WT mice (3 mice each) according to manufacturer's instructions using the RNeasy Micro kit (Qiagen), including a 30 min DNase I treatment at room temperature for removal of genomic DNA.

During the next couple of hours, the tubes are shaken, spun, heated, and cooled — all part of the process to extract RNA, the molecule that animals and viruses alike use to encode proteins.

After homogenizing these samples with a bead cell crusher (MS - 100; Tomy, Tokyo, Japan), total RNA was extracted using TRIzol reagent.

To validate the tissue specificity of temperature - induced Acks expression, total RNA was extracted from the brains, thoraxes, and abdomens of five heat - exposed intact workers and then subjected to quantitative RT - PCR.

Total RNA was extracted from the whole brains of Japanese honeybee workers with seizures induced by awakening from anesthesia, using TRIzol Reagent (Invitrogen, Carlsbad, CA).

Total RNA was extracted as previously described (Vohanka et al., 2010).

Human total RNA was also extracted using TRIzol ® Reagent (Invitrogen) from peripheral blood lymphocytes and fat tissue.

Total RNA was extracted from the retinal samples using RNeasy Lipid Tissue Mini Kit (Qiagen, Inc., Valencia, CA).

Target preparation and microarray hybridization — Total RNA was extracted from retinal samples using RNeasy Lipid Tissue Mini Kit (Qiagen) and was the substrate for amplification and labeling using a procedure based on the Eberwine protocol [49].

Total RNA (1 µg) was extracted (PicoPure RNA isolation kit; Thermo Fisher Scientific), and libraries were generated using the NEBNext Ultra Directional RNA Library Prep Kit (Illumina), size - selected for 200 - bp inserts, and quantified on an Agilent bioanalyzer using a DNA 1000 chip.

We work very closely with the Sequencing and Microarray Facility (SMF) and the DNA / RNA samples extracted from us can be submitted directly from our core to SMF across the hallway.

Total RNA from Cellartis enhanced hiPS - HEP cells from C12, C18, and C22 (n = 2 batches per cell line) was extracted on Day 13 post-thawing as well as from hphep cells (n = 3 donors) Day 1 post-thawing using the GenElute RNA / DNA / Protein Plus Purification Kit (Sigma Aldrich).

Total RNA was extracted from the cerebellar cultures at 14 and 16 DIV and renal fibroblast cultures using the RNeasy Mini Kit (Qiagen, Tokyo, Japan).

The Quantitect One - Step RT - PCR kit (Qiagen, Hilden, Germany) was used with a 25 μl reaction mixture under the following conditions: 0.25 μl of kit enzyme mixture (including reverse transcriptase RT and Taq polymerase), 10 μl of 2 × Quantitect RT - PCR buffer, 1.25 μl of 10 μM of each primer, 0.5 μl of 10 μM of probe at 10 μM, 6.8 μl of DNA RNA free water (Mol Bio grade, Hamburg, Germany) and 5 μl of the extracted sample.

RNA was also extracted from supernatant of mosquitoes pools as described above and used for the ZIKV rRT - PCR assay.

All tissues were collected from the Uppsala Biobank and RNA samples were extracted from frozen tissue sections.

Total RNA was extracted using TRIzol (Invitrogen) from either the fat body or whole body.

The extracted total RNA was subjected to two separate reactions — one to synthesize cDNA and the other to test for DNA contamination.

The concentration and purity of extracted RNA were determined by measuring the A260 and A280.