Sentences with phrase «rna from»

In order to know what parts of the code are used to regulate cell functions and create traits the RNA from the organism needs to be sequenced.

Students will also transcribe a messenger RNA from their original DNA template.

Oral administration of Eurocel resulted in the reduction of HCV RNA from 1 X 108 or 1 X 109 to 1 X 103 with oral administration of Eurocel.

The total RNA from the adipocytes was isolated and carried out GeneChip microarray analysis.

Total RNA from 8 to 18 embryos was extracted using Omega E.Z.N.A Total RNA Kit (Norcross, Georgia).

Deep sequencing of cerebral white matter - derived RNA from the HIV (n = 4) and ODC (n = 4) patients and SURG (n = 2) groups revealed bacterially - encoded 16 s RNA sequences in all brain specimens with α - proteobacteria representing over 70 % of bacterial sequences while the other 30 % of bacterial classes varied widely.

However, if certain smaller molecules of RNA — called microRNAs — bind to a messenger RNA molecule, they «silence» it, which prevents the information in the messenger RNA from being translated to make a protein.

In other cases, histone modifications may block RNA from transcribing DNA and silence a gene.

The results of this study support the existence of four distinct subtypes of the disease, based on the patterns seen in the transcription of RNA from DNA.

Topic: DSSR, a new 3DNA program for Defining the Secondary Structures of RNA from three - dimensional coordinates Presenter: Xiang - Jun Lu, Ph.D., Associate Research Scientist, Columbia University Host: Jason Key Recorded on June 26, 2014

Frozen total RNA from any human sample and / or human derived cell - cultures.

Importantly, while the RNA from sample VP62 extracted in 2006 was positive for XMRV, RNA extracted from the same prostate tissue in 2012 was found to be negative not only by ViroChip and PCR (Fig. 2; Table 1) but also by deep sequencing, with no XMRV sequences detected out of 4 million deep sequencing reads (Fig. 5).

Malathi, K., Saito, T., Crochet, N., Barton, D.J., Gale, M.and Silverman, R.H. RNase L Releases a Small RNA from HCV RNA that Refolds into a Potent PAMP.

Researchers will analyze DNA and RNA from the samples to identify and catalog genomic variants and gene expression.

For negative control data where each droplet contains RNA from the same solution, the count depth variability must be technical, but in real samples this could also be due to cells having variable amounts of RNA.

RNA from prostate cancer tissues corresponding to the 39 individuals identified in the prospective study was initially extracted in a separate mouse - free, XMRV PCR amplicon - free laboratory (University of California, San Francisco).

Total RNA from monkey tissues was extracted using Sepasol RNAI (Nacalai Tesque, Kyoto, Japan) according to the manufacturer's instructions.

When sequencing RNA from individual cells finally became possible, the initial analyses were what Hacohen called «biased» and «shallow» because the few markers used to classify the cells were too insensitive to nuances of differences among them.

To compare the expression estimates form RNAseq and microarrays to those from qRT - PCR, RNA from OM and VNO was extracted, as previously described, from four individual male and four individual female C57BL / 6J mice.

Full length transcripts of Lcn16 and Lcn17 were obtained using 5 ′ and 3 ′ RACE kits (Invitrogen) on RNA from VNO and OM tissue following the manufacturer's instructions.

RT - PCR analysis of RNA from mouse embryonic and adult gonads using primers corresponding to either exons 3 and 9 (upper panel, 501 - bp band) or exons 3 and 12 (lower panel, 847 - bp and 712 - bp bands) reveals that full - length Bol1 is only expressed in postnatal testes, while Bol2 lacking exon 11 is also detectable at low levels throughout gonadal development in addition to its high adult testis expression.

Total RNA from ES cells or EBs was isolated using the RNeasy Mini Kit (Qiagen, Hilden, Germany).

Total RNA from mutant and wild - type ovaries was isolated using Trizol (Invitrogen).

By sequencing RNA from infected and uninfected control samples and identifying genes that are differentially regulated by infection status, it is possible for the first time to identify all genes regulated by a specific immune challenge independent of homology - based annotations.

Total RNA from whole larvae or larval tissues was extracted using a PureLink RNA Mini Kit (Life Technologies).

The microbe was able to make RNA from them, the first step in translating the information in genes into proteins.

In the second experiment, RNA from several mouse tissues was amplified with ospA primers.

They are expressed as messenger RNA from which protein is made.

One point two micrograms of total RNA from each cell line was reverse - transcribed using random primers and the High - Capacity cDNA Synthesis Kit (Applied Biosystems, Foster City, CA, USA).

In addition, analysis of the microarray results obtained with RNA from the optimized protocol showed that, as expected, the genes involved in methane production (methanogenesis) were among the most highly expressed genes in M. barkeri.

In order to know what parts of the code are used to regulate cell functions and create traits the RNA from the organism needs to be sequenced.

Ten nanograms of total RNA from each cell line was converted to cDNA using the High - Capacity cDNA synthesis kit (Applied Biosystems).

A PCR fragment containing full - length env and rev genes was derived from plasma virion - associated RNA from a subject infected with a cla

A PCR fragment containing full - length env and rev genes was derived from plasma virion - associated RNA from a subject infected with a clade B virus by reverse transcription and nested PCR amplification procedures.

Total RNA from Cellartis enhanced hiPS - HEP cells from C12, C18, and C22 (n = 2 batches per cell line) was extracted on Day 13 post-thawing as well as from hphep cells (n = 3 donors) Day 1 post-thawing using the GenElute RNA / DNA / Protein Plus Purification Kit (Sigma Aldrich).

A PCR fragment containing full - length env and rev genes was derived from plasma virion - associated RNA from a subject acutely infected with a clade B virus by reverse transcription and nested PCR amplification.

mRNA expression from the targeted allele could be detected in embryonic stem cells by RT - PCR (Figure 1D), although expression was reduced approximately 5-fold relative to wt mRNA transcript levels as determined by Northern blotting of RNA from the testis of heterozygous animals (Figure 1E).

First, the team sequences DNA and RNA from tumor biopsies and inputs that information into a computer model that identifies a handful of dysregulated proteins necessary for survival of the tumor cell, what Califano calls the «master regulators.»

AMSBIO offers a range of kits and reagents for the extraction (isolation) of high quality RNA from FFPE tissues and LCM samples that can be used not only for PCR but also microarray applications.

Sovaldi neutralizes an RNA polymerase — an enzyme that transcribes RNA from DNA.

RNA from 40 EF, PNP, LE dissected tissues (approximately 250 µm2 each) or ten YFP - expressing control or YFP - and EFTF - expressing animal caps (EFTF - expressing pluripotent cells) were used for each chip analysis.

Isolate RNA from each collected cell population using TRI reagent following manufacturer's instructions.

A paper published last April in Science detailed how geneticists sequenced the RNA from 100 strains of rhinovirus — all the known types of the leading cause of the cold.

The NYGC and its founding member institutions are conducting additional studies involving Watson to help accelerate the discovery of potentially actionable sequence variants in various types of cancer, including an ongoing study that involves DNA and RNA from a larger cohort of glioblastoma patients, and a study of 200 patients with different types of cancer.

Total RNA from single animals was individually converted into biotinylated, fragmented cRNA using protocols recommended by the microarray manufacturer (Affymetrix Inc., Santa Clara, California, USA).

The sample purity (A260 / 280 and A260 / 230 ratios) of the isolated RNA from each sample will be reported.

In the study, NYGC researchers and bioinformatics experts analyzed DNA and RNA from a glioblastoma tumor specimen and DNA from the patient's normal blood, and compared potentially actionable insights to those derived from a commercial targeted panel that had previously been performed.

In the new eLife study, the researchers compared RNA from the cells of five different groups: salamander cells with algae, salamander cells without algae, the algal cells living in salamander cells, the algae living in the egg capsules, and algae cultured in the laboratory.

Making RNA from scratch is very hard,» Keller said.

In both experiments, they studied RNA from chimps, humans, and one of the other primates.