Sentences with phrase «rna gene sequences»

He hopes to test samples of martian soil for ancient ribosomal RNA gene sequences that are conserved in all of Earth's known life forms.

We examined 13,355 prokaryotic ribosomal RNA gene sequences from multiple colonic mucosal sites and feces of healthy subjects to improve our understanding of gut microbial diversity.

After six to eight weeks, the microbial content of the mice's feces were analyzed by 16S ribosomal RNA gene sequencing, which showed that approximately 20 kinds of oral bacteria were present in these mice.

In this study, we used ribosomal RNA gene sequencing to identify the zooxanthellae, bacteria and archaea associated with healthy and yellow band diseased (YBD) colonies in the Media Luna reef of La Parguera, Puerto Rico, in order to examine the influence of YBD on the Montastraea faveolata microbiome.

Not exact matches

Here's why: In the late 90s, technology emerged enabling researchers to rapidly sequence RNA and DNA — giving them the ability to isolate and record the activity of single genes.

Citation: Lemay DG, Ballard OA, Hughes MA, Morrow AL, Horseman ND, Nommsen - Rivers LA (2013) RNA Sequencing of the Human Milk Fat Layer Transcriptome Reveals Distinct Gene Expression Profiles at Three Stages of Lactation.

In addition, they also used exome sequencing and RNAseq — sequencing directly targeted to the SHIP1 gene — to examine the structure of the SHIP1 gene at both the DNA and the RNA level.

The vaccine can be targeted to the chosen pathogen by using RNA molecules which share sequence identity with the pest's genes and prevents their expression.

«Decoding the genome's cryptic language: New tool to map RNA - DNA interactions could help researchers translate gene sequences into functions.»

RNA molecules can attach to particular DNA sequences to help control how much protein these particular genes produce within a given time, and within a given cell.

These are sequences made in the lab from RNA — the template used to produce the proteins that genes code for.

Since messenger RNA is nothing but the imprint of the gene whose message it is carrying, it is possible to use it to chemically generate the DNA sequence or gene it corresponds to.

Each guide RNA might hit a single gene or multiple genes, depending on the particular guide sequence.

Using RNA sequencing, the researchers found multiple genes whose abnormal expression could lead to the high rate of death for cloned embryos, including failure to implant in the uterus and failure to develop a normal placenta.

To more accurately reflect the mechanisms driving oligodendrogliomas, the researchers used RNA sequencing to study directly, on a single - cell level, gene expression in samples from six early - stage human tumors.

MicroRNAs are short RNA sequences that turn genes on or off.

Using a technique known as single - cell RNA sequencing, the team explored more than 65,000 individual cells that exist under normal or inflammatory conditions, looking for genes that were more active in one state or subpopulation versus another.

In germline cells PIWI proteins silence the RNA from jumping genes by cutting them in sequences of ~ 30 nucleotides that will become piRNAs.

The pilot project tested a dozen or so of the most commonly used gene promoters (regions of DNA that facilitate gene transcription) and segments of DNA that encode ribosome - binding sites (sequences of messenger RNA that control protein translation) to determine whether they behave consistently in different cellular contexts.

Collaboratively, the research team sequenced the DNA that encodes the genes as well as the RNA from the insect at different developmental stages, to identify when different genes are expressed and in which tissues and organs.

So to reveal their function, biologists used the sequence information along with a technique called RNA - mediated interference (RNAi) to temporarily inactivate each of nearly 17,000 of the worm's genes.

First described in the 1990s — a discovery that led to the 2006 Nobel Prize — RNAi is a process by which organisms suppress the expression of target genes through the action of small RNA segments that bind to corresponding gene sequences.

«Single - nucleus RNA sequencing, droplet by droplet: DroNc - Seq, technology that merges single - nucleus RNA sequencing with microfluidics, brings new scale to gene expression studies in complex tissues.»

By comparing proteomic and RNA - sequencing data from people on different exercise programs, the researchers found evidence that exercise encourages the cell to make more RNA copies of genes coding for mitochondrial proteins and proteins responsible for muscle growth.

The gene - editing success appears to be largely due to one procedural change: The researchers introduced the editing system — the enzyme Cas9 and a guide RNA sequence that helps the editing machinery find its target — at the same time they injected the mutation - laden sperm into a healthy egg in the lab.

RNA sequencing of both single and clustered CTCs from breast cancer patients identified several genes expressed at elevated levels in CTC clusters, one of which — a protein called plakoglobin — also was overexpressed in the primary tumors of patients with reduced survival.

Our RNA sequencing analysis of NAFLD - HCC samples revealed squalene epoxidase (SQLE) as the top outlier metabolic gene overexpressed in NAFLD - HCC patients.

The technique involves designing double - stranded RNA molecules that match a sequence in the RNA produced by the gene you want to inactivate.

This sequence always inserts its DNA next to genes that are «read» by an enzyme called RNA polymerase III.

Combining this mouse model with the reliable RNA sequences allowed an efficient inactivation of genes in primary cells.

In order to locate all gene switches, the Freiburg research team used modern sequencing methods to examine the entire genome — DNA, epigenetic markers and RNA — during the development, maturation and disease of human cardiac muscle cells.

Using next - generation RNA sequencing and other advanced technologies, the researchers identified a previously unknown gene involved in betalain synthesis and revealed which biochemical reactions plants use to convert the amino acid tyrosine into betalains.

They also showed that they could block up to five genes at once by delivering different RNA sequences.

Microbiota or microbiome structure, gene expression, and metabolism were assayed by 16S ribosomal RNA profiling, whole - community shotgun sequencing, RNA - sequencing, and mass spectrometry.

By using sequences of RNA that match specific gene sequences in a pest, RNAi should leave other species unscathed.

In vitro experiments and high - throughput RNA sequencing revealed that CTCF deletion profoundly altered the B lymphocyte transcriptional program, shifting cells from a gene expression profile typical of the germinal center to one more similar to that seen in plasma cells.

Technologies such as RNA sequencing are revealing which genes are expressed in each individual cell.

By using random series of 30 nucleotides — the building blocks of RNA and DNA — he thought he could give neurons unique barcodes, linking the barcodes across synapses, then create maps of their connections using high - throughput gene sequencing technology.

In CRISPR - Cas9 gene editing, a guide RNA sequence (green) helps Cas9 protein (purple) cut DNA at the correct spot.

First, samples of leaves from these plants are collected for in vitro cultures to isolate the fungi; then the DNA and RNA of fungi are extracted to sequence them and, through bioinformatic analysis, the researcher can determine the expression, the presence or absence of genes in the genomes of a species against each other.

Subsequent RNA sequencing suggested that digoxin inhibits HIV - 1 gene expression as well as the activation and metabolism of CD4 + T cells.

Surprisingly, they found that although the patterns of gene expression — as shown by the RNA sequencing — differed between the hepatocellular carcinomas and the liver cancers with biliary phenotype and depended on the histological type, the overall pattern of mutations in the cells was actually similar between the tumors — of either type — that had emerged in patients who had had infections with either hepatitis C or B, and were different in patients without such infections.

Extensive research has already examined the function of microRNAs, a category of small evolutionarily conserved noncoding RNAs about 22 to 24 nucleotides in length that target protein - coding genes in a sequence - specific manner.

The Rutgers scientists show that the transcription activator protein functions by binding to a specific DNA sequence preceding the target gene and making adhesive, Velcro - like interactions with RNA polymerase that stabilize contacts by RNA polymerase with adjacent DNA sequences.

To study gene expression, they then examined RNA sequencing data from 25 of the biliary - phenotype cancers and 44 hepatocellular cancers.

These are sequences produced in the lab from the RNA that is the intermediate step between gene and the protein it codes for.

Among the key elements engineers need to get an organism such as E. coli to make a protein from a synthesized gene are extra sequences such as promoters (to help the cell make RNA from DNA) and ribosome binding sites (or RBS, which the cell needs to make proteins from the RNA).

To develop fluorescent in situ sequencing, scientists first fix in place thousands of RNAs — including working copies of genes called messenger RNAs — in cells, tissues, organs or embryos.

In this way the team could create a composite image representing the sequence, and location, of RNA corresponding to every gene in the human genome.

The team, headed by OIST's Prof. Alexander Mikheyev of the Ecology and Evolution Unit and Dr. Claire Morandin, post-doctoral scholar at the University of Helsinki, collected queens and workers and sequenced the transcriptome — the full range of messenger RNA molecules expressed by an organism — of 16 species of ants to create a co-expression network of 36 sets of genes, that represent groups of similarly expressed genes.