Sentences with phrase «rna samples»

Gene expression profiles are provided for all RNA samples combined, or separately for the CGR8 and E14TG2a ES cell lines.

RNA samples were hybridized to two microarray platforms: Affymetrix U133A array (GPL96) and GN1H custom array (GPL1074), enabling testing 44775 human transcripts.

A limited number of total or polyadenylated (polyA) RNA samples (n = 21, corresponding to 14 unique samples) from the original 2006 study by Urisman, et al. [1], 6 previously found to be XMRV - positive and 8 XMRV - negative, were available for independent re-analysis.

The configuration of each of the eleven individual experiments and the RNA samples collected are summarized in Table 1.

Coded RNA samples from prostate cancer tissue biopsies were shipped from UCSF to the Cleveland Clinic.

The expected turnaround time will be 2 weeks for every 16 RNA samples plus an additional week for every other 16 samples.

After microarray analysis, available remaining material corresponding to 17 total and / or polyA RNA samples (13 unique samples) were tested for XMRV by gag RT - PCR.

The ViroChip microarray [2], [3], [44], [45] was used to screen RNA samples isolated from prostate tumors collected prospectively from 39 individuals, of which 16 individuals were genotyped as harboring the R462Q RNAse L mutation (QQ), 10 individuals were heterozygous cases (RQ), and 13 were wild - type cases (RR).

The 21 RNA samples were first analyzed using the ViroChip microarray.

Finally, RNA samples were prepared following the same procedure and subsequent microarray analyses were performed in a central facility using Affymetrix Mouse 430 v. 2 arrays.

The names of the 67 RNA samples are indicated on the top.

RT - PCR analysis of RNA samples from EBs on days 3, 6 and 9 revealed a gradual decrease in expression of endogenous pluripotency markers such as Nanog, Rex1, Oct4 and Sox2 as compared to their expression in undifferentiated iPS cells (Figure 4B, upper panel).

All tissues were collected from the Uppsala Biobank and RNA samples were extracted from frozen tissue sections.

The CNAG Biorepository collects, quantifies and stores the DNA and RNA samples for sequencing requests.

As applied to the Initiative, the enhanced sensitivity of the laser ablation measurement approach will be harnessed for measuring stable isotope content of nanogram amounts of RNA samples.

We work very closely with the Sequencing and Microarray Facility (SMF) and the DNA / RNA samples extracted from us can be submitted directly from our core to SMF across the hallway.

RNA samples were studied in triplicate (i.e., 3 microarray slides for each retina).

A typical gene expression experiment involves generating fluorescently labelled cDNA from two RNA samples.

MiRNA - seq and mRNA - seq will be carried out from the same RNA sample thereby integrating the mRNA and miRNA «sequenomes» of infected human cells most relevant to human toxoplasmosis.

mRNA was prepared for sequencing using the TruSeq RNA sample preparation kit (Illumina) with a selected fragment size of 200 — 500 bp.

Not exact matches

Using microbiome analysis technology first developed in Los Alamos Lab to combat disease warfare, the company takes the samples and creates RNA and metabolic profiles for each customer.

We found that: a) lactation stage must be defined biochemically (such as Na: K ratio), not by day of lactation, b) lactation stage influences RNA quality, quantity, and potential immune cell contamination, c) immediate processing and hard, fast centrifugation yield better quality RNA, and d) additional washing of the milk fat layer is not necessary for the analysis of mature milk samples and does not decrease immune cell contamination in colostrum samples.

Among colostrum samples, only 27 % of RNA isolates were suitable for sequencing (RIN ≥ 7.0 and RNA ≥ 10.0 ng / uL).

The researchers detected this SMN long noncoding RNA, or lnc - RNA (pronounced «link RNA») for short, in human embryonic kidney cells, brain cell samples and neurons derived from the stem cells of healthy people and those with spinal muscular atrophy type I and II.

While the latter has a reputation for being hard to isolate, she explains that even degraded RNA generally contains enough intact sequence to analyze — provided investigators can detect the scarce tumor signals against the immense background of other RNA molecules in a sample.

Nucleic acids are also helping scientists to detect biomarkers, such as Abcam's Fireplex (formerly Firefly) particle technology, which profiles biomarker microRNAs (miRNAs) directly from biofluid samples, without RNA purification.

Venter's samples could have been full of RNA - based life that would have slipped through his net.

Before he froze his samples, he added an RNA template — a single - strand chain of RNA that guides the formation of a new strand of RNA.

The assay was found to be 100 % sensitive and 100 % specific to ZIKV RNA from semen samples with a limit of detection of 5 viral copies per mL of semen.

So when Biebricher analyzed his own samples, he was amazed to see RNA molecules up to 400 bases long.

To more accurately reflect the mechanisms driving oligodendrogliomas, the researchers used RNA sequencing to study directly, on a single - cell level, gene expression in samples from six early - stage human tumors.

Complete viral RNA genome sequencing of ultra-low copy samples by sequence - independent amplification

Chan and colleagues are testing whether humans carry similar signs of stress in these RNA - loaded vesicles by studying college students» semen samples.

Then they compared the expression of thousands of genes in two samples by measuring levels of messenger RNA.

Alignments of RNA - seq data from three different studies across 18 samples have additionally been located on the new assembly.

Several companies now sell reagents and equipment for isolating RNA from individual cells while keeping the thousands of resulting samples separate.

One is being tested here as the midwife uses a finger stick to collect 300 microliters of blood, just five or six drops, in a small tube, then she treats the samples with a DNA / RNA shield, essentially detergent to prevent degradation.

In comparison to RNA from healthy brains, more of the RNA from Alzheimer's brain samples was unspliced.

The researchers analyzed the samples using immunohistochemistry and RNA sequencing to study the changes in the tumor immune microenvironment of patients who received and did not receive chemotherapy, and changes before and after chemotherapy.

This family tree captures just a sampling of single - cell and single - nucleus RNA sequencing technologies that have burst onto the scene over the last eight years.

Our RNA sequencing analysis of NAFLD - HCC samples revealed squalene epoxidase (SQLE) as the top outlier metabolic gene overexpressed in NAFLD - HCC patients.

The study divides 177 blood and 27 post-mortem brain samples into several groups, establishing that careful analysis of RNA transcripts in blood samples has the ability to distinguish early clinical AD, Parkinson's disease (PD), and cognitively healthy patients.

The Apollo 324 library preparation system can be used to process DNA and RNA from clinical samples to NGS - ready libraries.

«We've been collecting samples in a lot of places to analyze DNA and RNA,» Tringe says.

He notes, however, that the freezing and processing required to study the blood samples often causes degradation that makes RNA easier to find than the virus.

Further, investigation of these CpG associations revealed nearby genes whose RNA expression was altered in brain samples with Alzheimer's disease: ANK1, CDH23, DIP2A, RHBDF2, RPL13, RNF34, SERPINF1 and SERPINF2.

In this context, Nano - 85 was able to detect virus in one - third of the samples already known to be positive for noroviral RNA.

I put the RNA from both samples in a tube and mechanically shook it for three days behind plastic shields made from discarded Jack in the Box restaurant signs, because they were free and excellent at blocking radiation.

In Rice's experiments, samples were prepared with a commercial chemical kit that releases all the DNA and RNA in the small amount of stool tested.

The scientists were able to identify RNAs that were present at different levels in blood samples taken at those different testing visits, in common across these 37 individuals.