Sentences with phrase «rna splicing»

CCDC66 is evolutionarily conserved in different vertebrate species and exhibits a complex pattern of differential RNA splicing resulting in various isoforms in the retina.

To understand the molecular basis of these observations, we analyzed global RNA splicing in Sf3b1 ^{K700E / +} hematopoietic cells.

The two studies link the loss of the SMN1 gene to defects in RNA splicing of a critical neuronal gene, which leads to motor circuit dysfunction.

The therapy is an 18 - letter string of DNA that corrects aberrant splicing by binding a unique sequence on SMN2 «s messenger RNA located just downstream of the crucial seventh exon, thereby obstructing components of the RNA splicing machinery (see «Bring to one's antisenses»).

U1 Small Nuclear Ribonucleoprotein Complex and RNA Splicing Alterations in Alzheimer's Disease.

These compounds also corrected SMN2 RNA splicing and increased SMN protein levels in cell cultures obtained from SMA patients, including stem cell - derived motor neurons.

Investigates how RNA splicing contributes to breast and ovarian cancer progression, metastasis and drug - resistance.

Functional analysis of genes with recurrent exons (i.e., with association with PMI in more than two tissues) shows a noteworthy enrichment on RNA binding and RNA splicing genes (Supplementary Fig. 18b, c).

Multiple transcripts arising from alternative initiation or polyadenylation sites, or alternative RNA splicing at a single locus were not considered as multiple genes but as variants of a single transcription unit.

Single - Molecule Imaging of RNA Splicing in Live Cells.

Dr. Phillip Sharp's research has centered on the molecular biology of gene expression relevant to cancer and the mechanisms of RNA splicing.

His landmark achievement was the discovery of RNA splicing in 1977, for which he shared the 1993 Nobel Prize in Physiology or Medicine with Dr. Richard Roberts, who did parallel work at Cold Spring Harbor Laboratory.

«Our strategy to treat SMA relies on a simple, powerful antisense method that boosts SMN protein levels by fixing a genetic RNA splicing glitch.

Harvard researchers have found a link between the RNA splicing process and aging, which could lead to new treatments in helping humans live longer and healthier lives (Credit: halfpoint / Depositphotos)

Our genes provide the blueprints for producing the required proteins, and one of the main parts of that process is RNA splicing, where RNA molecules are cut and reconnected to allow for more varied proteins to be expressed.

Now, a Harvard team has discovered a link between aging and a core biological process known as RNA splicing, which may be manipulated to not only increase our lifespan, but help us stay healthier for longer.

RNA splicing antisense technology allows researchers to influence the ultimate structure and function of proteins.

Proteins are synthesized from instructions coded in the DNA through a multi-step process that includes RNA splicing.

March 13, 2007 — Cold Spring Harbor, New York — RNA splicing antisense technology studied at Cold Spring Harbor Laboratory (CSHL) effectively corrected an mRNA splicing defect found in spinal muscular atrophy (SMA) patients, and is now ready to be tested in mouse models.

Harvard researchers have found a link between the RNA splicing process and aging, which could lead to new treatments in helping humans live longer and healthier lives

«These are fascinating results, and suggest that variability in RNA splicing might be one of the smoking guns of the aging process,» says Mair.

Site - directed mutagenesis and kinetic analysis of RNA splicing were used to identify a base triple in the conserved core of both a cyanobacterial (Anabaena) and a eukaryotic (Tetrahymena) group I intron.

The researchers utilized a technique known as exon skipping, a form of RNA splicing, to eliminate the portion of one of the IgE receptor gene's mRNA that is essential to making a protein which places the IgE receptor on the mast cell surface.

Thus, the team has demonstrated a novel, positive role for PKR activation and eIF2 - alpha phosphorylation in human globin RNA splicing, in contrast to the long - standing negative role of this intracellular stress response in protein synthesis.

Although this line of research is far from contributing to changes in the clinic, the team now has a much better understanding of how RNA splicing is controlled in the ear.

«RNA splicing machinery offers new drug target.»

But how do alterations in RNA splicing result in hearing loss?

«It is already known that Rbfox is important for controlling RNA splicing but what is not understood is its contribution to neuronal maturation and which specific cellular phenotypes it controls,» says Dr. Zhang.

«By being able to distinguish benign from malignant aging based on distinctive RNA splicing patterns, we can develop therapeutic strategies that selectively target leukemia stem cells while sparing normal hematopoietic stem cells,» she said.

«Our findings show that RNA splicing is a unique therapeutic vulnerability for secondary AML,» said Jamieson.

The authors say it's the first study to show RNA splicing modulators inhibit cancer stem cell activity.

If disrupted, RNA splicing could enhance the capacity of cells to propagate cancer.»

Using sensitive genetic sequencing technology, the scientists identified unique RNA splicing variants that distinguish normal, aging stem cells from abnormal, malignant ones.

Crews noted that RNA splicing - targeted agents have been shown to have activity in a variety of solid tumors so the findings may be relevant to a variety of cancers, such as breast and drug - resistant melanoma.

Shinde and Klein are not yet sure whether GSK - 3's effect on RNA splicing explains its role in mood disorders.

«Alzheimer's: Newly identified protein pathology impairs RNA splicing.»

Also on the list were several «U1 snRNP» proteins, which are involved in RNA splicing.

What makes these tangles distinct is that they sequester proteins involved in RNA splicing, the process by which instructional messages from genes are cut and pasted together.

«We were very surprised to find alterations in proteins that are responsible for RNA splicing in Alzheimer's, which could have major implications for the disease mechanism,» says Allan Levey, MD, PhD, chair of neurology at Emory University School of Medicine and director of the Emory ADRC.

A form of genetic variation, called differential RNA splicing, may have a role in tumor aggressiveness and drug resistance in African American men with prostate cancer.

As a consequence of differential RNA splicing, mRNAs containing different combinations of exons can be produced from the same gene.

Like Kornblihtt's lab, alternative RNA splicing makes use of constrained resources in innovative ways.

In fact, he and his community of researchers in alternative RNA splicing — a field he helped to create — have shown that they can do world - class research despite tight government budgets and three - month delivery times for reagents that can cost three times as much as they would in the United States or Europe.

Nor is RNA quite so inert or structurally constrained as its cousin; its conformational versatility and catalytic abilities have been implicated at the very core of protein synthesis and possibly of RNA splicing.

Coupling of Tetrahymena ribosomal RNA splicing to beta - galactosidase expression in Escherichia coli

The beta - galactosidase messenger RNA splicing system provides a useful method for screening for splicing - defective mutations, several of which have been characterized.

Not exact matches

(Ligases are enzymes that splice together other molecules such as DNA or RNA.)

Thus RNA self - splicing can occur at a rate sufficient to support gene expression in a prokaryote, despite the likely presence of ribosomes on the nascent RNA.

The chemical mechanisms of RNA - catalyzed reactions are discussed with emphasis on the self - splicing ribosomal RNA precursor of Tetrahymena and the enzymatic activities of its intervening sequence RNA.

Splicing in vitro of a messenger RNA (mRNA) precursor (pre-mRNA) is inhibited by a monoclonal antibody to the C proteins (anti-C) of the heterogeneous nuclear RNA (hnRNA)- ribonucleoprotein (hnRNP) particles.