Sentences with phrase «rpe cell markers»

We compared the expression of RPE cell markers in transplanted cells in vivo against cells re-plated and cultured in parallel.

(D) Expression of the same RPE cell markers is maintained in vivo by iPS - RPE (white arrows) 8 days following transplantation into the subretinal space of the RCS rat.

(E) Western blot analysis of iPS - RPE protein expression using antibodies against a panel of RPE cell markers.

Not exact matches

The differentiated RPE cells exhibit native characteristics including morphology, pigmentation, marker expression, monolayer integrity, polarization and phagocytic activity.

(B) High power image showing pigment granule - containing donor cells in the RPE - L region that are positive for human nuclear marker (arrows).

The pigmented subretinal donor cells failed to express two characteristic RPE markers, RPE65 [57] and bestrophin [58], arguing against the possibility that they had undergone full transdifferentiation.

(E) Rhodopsin - positive material (red) is present within the cell membrane of human specific marker (HSM)- labelled iPS - RPE (green) 8 days post-transplantation and in the tips of the host outer segment (OS) layer.

Confocal microscopy through cells labelled with the apical marker ATP1B1 demonstrated that POS are internalized by iPS - RPE (Fig. 4A and Movie S1).

We found that differentiated iPS - RPE cells were morphologically similar to, and expressed numerous markers of developing and mature RPE cells.

However, the transplanted cells maintained expression of RPE markers such as RLBP1 and OTX2, with only occasional cells positive for Ki67.

After 8 days in culture, the re-plated iPS - RPE cells were fixed and stained for RPE - associated markers (Fig. 5C).

Internalization of POS is observed in a single optical y - axis projection (< 1 µm) of pigmented iPS - RPE cells labelled with the apical cell surface marker ATP1B1 (red).

(C) Retention of RPE markers by iPS - RPE cells in vitro after dissociation, re-plating and culturing for 8 days.