«People say, «If it looks like life and has
a Raman signal of carbon, then we have life,» says Marshall.
The device enhances the sensing properties of the technique by creating «hot spots,» or narrow gaps within the nanostructure which intensified
the Raman signal, the researchers said.
To obtain molecular orientation information from the coherent
Raman signal, the researchers used an electro - optical device called a Pockels cell to quickly modulate the laser's polarization rather than its intensity.
A recently developed enhancement technique uses pristine graphene as a substrate, which can enhance
the Raman signal by several orders of magnitude.
On its own,
the Raman signal is so weak that many methods have been used to enhance the signal.
In the 1970s, researchers found that chemically roughening the surface of a silver substrate concentrated
the Raman signal of the material adsorbed on the silver, and SERS was born.
But if all the molecules can be clustered among the gold nanoparticles, they will produce a very strong
Raman signal.
«People say, «If it looks like life and has
a Raman signal of carbon, then we have life,»» Marshall said.
«The test with the bulk sample showed us that
the Raman signals can be used as a kind of the fingerprint of phase transition at temperatures around 118 Kelvin, or minus 155 degrees Celsius.
«There are some molecules that must be responsible for these different
Raman signals, but we don't need to know what those molecules may be,» Mohan said.
«We are proposing the nephrologist will puncture the patient's skin, go to the surface of the kidney, and not puncture the kidney, but probe the surface of the tissue and acquire
Raman signals,» Mohan said.
Not exact matches
In surface - enhanced
Raman spectroscopy, nanoparticles are added to boost the
signal.
But
Raman spectroscopy suffers from a major drawback — the scattered
signal is weak, meaning that light is ordinarily difficult to collect even tens of meters away from a sample.
Bond and her collaborators are using metal - coated nanotubes bunched together like a jungle canopy to amplify the
signals of both the incident and
Raman scattered light by exciting local electron plasmons.
Raman has spent a decade learning how the human brain and olfactory system operate to process scent and odor
signals.
The scientists worked with model bacteria that are representative of the sort of microbial lifeforms that might be expected to have emerged on Mars and used a
Raman spectrometer to track how the detectable
signal from them changed with increasing exposure to radiation.
Fluorescent dyes, which are frequently used as markers in biological experiments, are particularly hard to detect in
Raman spectroscopy because the fluorescence tends to wash out the
signal.
«The
signal detection for our technique is very similar to what is done with stimulated
Raman scattering, except that instead of detecting only the intensity of the light, we detect polarization information that tells us if molecules are highly oriented or totally disorganized.»
Shih's research team developed a metric to broadly quantify the level of disease using the
Raman scattering
signals.