Sentences with phrase «sapap3 knockout mice»

Oxytocin receptor in the Hypothalamus is Sufficient to Resue Normal Thermoregulation Function in Male Oxytocin Receptor Knockout Mice.

As they studied brain activity in the knockout mice, the researchers also found prominent changes in a receptor in the brain known as mGluR5 and other proteins that support the function of neurons and synapses, said co-lead author Xiaoming Wang, M.D., Ph.D., senior research associate in Duke's department of pediatrics.

Over the past five years, researchers observed abnormal brain activity in the SHANK3 knockout mice when compared to controls.

Curious control mice roamed while knockout mice often stuck to a small area and repeated the same routes.

p11 knockout mice exhibit a depression - like phenotype and have reduced responsiveness to 5 - HT1B receptor agonists and reduced behavioral reactions to an antidepressant.

The knockout mice, which will be made available to academic researchers at low cost, should help efforts to figure out what newly discovered genes do in the body.

«Many labs have the expertise that would allow them to exploit knockout mice, but the main hurdle has been the cost,» says endocrinologist Joseph Majzoub of Children's Hospital in Boston.

We generated knockout mice lacking synaptobrevin / VAMP 2, the vesicular SNARE protein responsible for synaptic vesicle fusion in forebrain synapses, to make use of the exquisite temporal resolution of electrophysiology in measuring fusion.

We generated knockin / knockout mice that expressed only open syntaxin - 1B.

Wherry and his collaborators used PD - 1 knockout mice infected with lymphocytic choriomeningitis virus to see whether the genetic deletion of PD - 1 would be enough to prevent T cell exhaustion.

Currently, it takes up to 10 months — and as much as $ 100,000 — to develop a knockout mouse strain.

But a few weeks after birth, these so - called «knockout mice» spontaneously developed serious inflammation and tissue damage in several organs (see photo).

Based primarily at Harvard with Maniatis, tenOever developed a knockout mouse model to study a particular protein, IKKε, that's involved in the immune response to viral infection.

Looking forward, the researchers plan to investigate knockout mice lacking the gene that produces AGF to further define its role.

Knockout mice, which completely lacked the gene and so manufactured no mu opiate receptors, were all the more sensitive.

Days later, when these knockout mice were given the same tasks, the animals regained their expertise faster and more fully than ordinary mice with strokes.

In the knockout mice, the virus replicated to higher levels than in those treated with the antibody, killing most of the fetuses.

Although the Nppb - knockout mice lived a normal lifespan, when the researchers killed the receptor cells in the spinal cord, the mice died prematurely, suggesting that it could be dangerous to try humans, Hoon says.

«There have been many knockout mouse models of cleft palate, but very few of cleft lip, which is actually the more common defect in humans,» Carstens said.

He also hopes that the Esrp1 - knockout mice will prove to be a valuable new model for studying cleft lip.

«The next thing to do is to generate a knockout mouse model for further in vivo analysis, as the ultimate goal of our research is to move from bench to bedside, and generating a mouse model is a key step during this long journey,» Shi said.

Normally, these substances make mice scratch nonstop, but the knockout mice hardly scratched themselves at all after the injection, showing that Nppb was required to produce the sensation of itch, the authors report online today in Science.

Although AMTN is expressed in the junctional epithelium, the knockout mice appear to have an intact dentogingival attachment.

Because mice lacking both genes would not be born alive, the scientists followed up this lead by making «conditional knockout mice,» in which Esrp1 and Esrp2 activity was normal early in fetal development, but then was switched off in skin epithelial cells.

As expected, gp96 knockout mice grew normally at first.

No other sensory systems appeared to be affected in the knockout mice, Hoon says.

Some of these, based on previous studies of knockout mice, seem to play a role in cognition and behavior, including fear responses and the ability to learn new behaviors when given food rewards.

They injected both the knockout mice and normal mice with AAV vector and found that 33 % -56 % had liver tumors, compared to 4 % -8 % of untreated mice.

Both behavioral and physiological experiments revealed that the Ggamma13 knockout mice did not respond to odors.

«In male knockout mice we noticed something quite startling,» Levine says.

These genetic modification techniques debuted in the 1980s, but the first autism knockout mice took a while longer to develop, emerging only in the early 2000s.

In an attempt to increase sensitivity for sepsis, Canaan aged these FAT10 knockout mice and made the discovery that mice lacking the gene were lean and aged more slowly.

In fact, whereas 74 percent of the control mice committed infanticide after the birth of their first litter, none of the knockout mice killed their offspring.

«Because BAI1 is very active in the hippocampus, a region of the brain critical for learning and memory, we decided to look at the performance of the knockout mice on a memory task dependent on the hippocampus,» Van Meir says.

Zeroing in on this kinase was encouraging, Goga said, because other researchers have shown that genetic - knockout mice that lack the entire family of PIM kinases are slightly smaller than normal mice, but «basically fine,» indicating that a drug targeting just PIM1 may have manageable levels of toxicity in breast cancer patients.

They found that the knockout mice had normal levels of leptin and ghrelin, suggesting that the loss of Magel2 alone leads to the disruption of hypothalamic feeding circuits, an effect independent of the effects of those metabolic hormones.

However, in response to TBI, the knockout mice showed greatly reduced astrocyte reactivity compared with controls.

This achievement is expected to become a very useful technology for future mass production of knockout mice.

The average number of eggs for genetically modified mice (knockout mice) obtained using previous methods of superovulation induction is about 20 but in reality the number is often much smaller, about 10 or less.

The use of an inhibin antiserum promotes excessive secretion of FSH by neutralizing inhibin in the body of female knockout mice.

The scientific community uses knockout mice to analyze basal phenotypes (the appearance of genetic characteristics) to analyze the function of such a large number of genes.

In this study, by using a systemic mtEF4 gene knockout mouse model, researchers found that mtEF4 knockout damages the oxidative phosphorylation function in germ cells of male mice, thus causing male sterility.

The knockout mice also did better on tests of behaviors associated with the brain's prefrontal cortex, the area that regulates complex thinking, emotions, and behavior in humans.

For this reason, efficient production of knockout mice is necessary.

When these knockout mice were fed a high - fat diet, they gained as much weight as normal mice did, but they managed to remain sensitive to insulin.

There is a new contender in the field of gene discovery, and it's giving knockout mice a run for their money.

«With our newly developed method, it is possible to reduce the number of female knockout mice used in egg collection by 1/3 — 1/4 or less.»

In another experiment, the researchers tested whether MMP - 9 has a functional role in ALS by crossing MMP - 9 knockout mice with SOD1 mutant mice (a standard mouse model of ALS).

An international team of researchers has identified a cause for chronic bad breath (halitosis), with the help of gene knockout mice from the UC Davis Mouse Biology Program.

Eliminating genes in mice is a standard technique for determining their function; the resultant animals are called knockout mice.